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A mutation in the melon Vacuolar Protein Sorting 41prevents systemic infection of Cucumber mosaic virus

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A mutation in the melon Vacuolar Protein Sorting 41prevents systemic infection of Cucumber mosaic virus

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dc.contributor.author Giner, A. es_ES
dc.contributor.author Pascual, L. es_ES
dc.contributor.author Bourgeois, M. es_ES
dc.contributor.author Gyetvai, G. es_ES
dc.contributor.author Rios, P. es_ES
dc.contributor.author Picó Sirvent, María Belén es_ES
dc.contributor.author Troadec, C. es_ES
dc.contributor.author Bendahmane, A. es_ES
dc.contributor.author García-Mas, Jordi es_ES
dc.contributor.author Martin-Hernandez, A.M. es_ES
dc.date.accessioned 2020-07-30T03:34:58Z
dc.date.available 2020-07-30T03:34:58Z
dc.date.issued 2017-09-05 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10251/148889
dc.description.abstract [EN] In the melon exotic accession PI 161375, the gene cmv1, confers recessive resistance to Cucumber mosaic virus (CMV) strains of subgroup II. cmv1 prevents the systemic infection by restricting the virus to the bundle sheath cells and impeding viral loading to the phloem. Here we report the fine mapping and cloning of cmv1. Screening of an F2 population reduced the cmv1 region to a 132 Kb interval that includes a Vacuolar Protein Sorting 41 gene. CmVPS41 is conserved among plants, animals and yeast and is required for post-Golgi vesicle trafficking towards the vacuole. We have validated CmVPS41 as the gene responsible for the resistance, both by generating CMV susceptible transgenic melon plants, expressing the susceptible allele in the resistant cultivar and by characterizing CmVPS41 TILLING mutants with reduced susceptibility to CMV. Finally, a core collection of 52 melon accessions allowed us to identify a single amino acid substitution (L348R) as the only polymorphism associated with the resistant phenotype. CmVPS41 is the first natural recessive resistance gene found to be involved in viral transport and its cellular function suggests that CMV might use CmVPS41 for its own transport towards the phloem. es_ES
dc.description.sponsorship The TILLING platform is supported by the Program Saclay Plant Sciences (SPS, ANR-10-LABX-40) and the European Research Council (ERC-SEXYPARTH). This work was supported by grants AGL2009-12698-C02-01 and AGL2012-40130-C02-01 from the Spanish Ministry of Science and Innovation, the Spanish Ministry of Econom and Competitiveness, through the "Severo Ochoa Programme for Centres of Excellence in R&D" 2016-2019 (SEV-2015-0533)" and the CERCA Programme/Generalitat de Catalunya. es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Secretory pathway es_ES
dc.subject Recessive resistance es_ES
dc.subject Plant-Viruses es_ES
dc.subject Tethering complexes es_ES
dc.subject Movement Protein es_ES
dc.subject Sequence es_ES
dc.subject Gene es_ES
dc.subject Transport es_ES
dc.subject Hops es_ES
dc.subject.classification GENETICA es_ES
dc.title A mutation in the melon Vacuolar Protein Sorting 41prevents systemic infection of Cucumber mosaic virus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41598-017-10783-3 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/341076/EU/Unraveling sex determination and parthenocarpy mechanisms to improve crops/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//SEV-2015-0533/ES/AGR-CONSORCI CSIC-IRTA-UAB CENTRE DE RECERCA EN AGRIGENOMICA (CRAG)/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ANR//ANR-10-LABX-0040/FR/Saclay Plant Sciences/SPS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2009-12698-C02-01/ES/Caracterizacion De Genes Y Qtls Implicados En Dos Caracteres Agronomicamente Relevantes En Melon: Resistencia Al Virus Del Mosaico Del Pepino Y Morfologia Del Fruto/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2012-40130-C02-01/ES/DISECCION GENETICA DE CARACTERES DE INTERES AGRONOMICO EN MELON: RESISTENCIA A CMV Y MADURACION CLIMATERICA DE FRUTO./ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Giner, A.; Pascual, L.; Bourgeois, M.; Gyetvai, G.; Rios, P.; Picó Sirvent, MB.; Troadec, C.... (2017). A mutation in the melon Vacuolar Protein Sorting 41prevents systemic infection of Cucumber mosaic virus. Scientific Reports. 7:1-12. https://doi.org/10.1038/s41598-017-10783-3 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41598-017-10783-3 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.identifier.pmid 28874719 es_ES
dc.identifier.pmcid PMC5585375 es_ES
dc.relation.pasarela S\346333 es_ES
dc.contributor.funder Saclay Plant Sciences es_ES
dc.contributor.funder Agence Nationale de la Recherche, Francia es_ES
dc.contributor.funder Generalitat de Catalunya es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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