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A Recombinant Potato virus Y Infectious Clone Tagged with the Rosea1 Visual Marker (PVY-Ros1) Facilitates the Analysis of Viral Infectivity and Allows the Production of Large Amounts of Anthocyanins in Plants

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A Recombinant Potato virus Y Infectious Clone Tagged with the Rosea1 Visual Marker (PVY-Ros1) Facilitates the Analysis of Viral Infectivity and Allows the Production of Large Amounts of Anthocyanins in Plants

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dc.contributor.author Cordero, Teresa es_ES
dc.contributor.author Mohamed, M.A. es_ES
dc.contributor.author Lopez Moya, J.J. es_ES
dc.contributor.author DAROS ARNAU, JOSE ANTONIO es_ES
dc.date.accessioned 2020-07-30T03:35:48Z
dc.date.available 2020-07-30T03:35:48Z
dc.date.issued 2017-04-06 es_ES
dc.identifier.issn 1664-302X es_ES
dc.identifier.uri http://hdl.handle.net/10251/148912
dc.description "This Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission." es_ES
dc.description.abstract [EN] Potato virus Y (PVY) is a major threat to the cultivation of potato and other solanaceous plants. By inserting a cDNA coding for the Antirrhinum majus Rosea1 transcription factor into a PVY infectious clone, we created a biotechnological tool (PVY-Ros1) that allows infection by this relevant plant virus to be tracked by the naked eye with no need for complex instrumentation. Rosea1 is an MYB-type transcription factor whose expression activates the biosynthesis of anthocyanin pigments in a dose-specific and cell-autonomous manner. Our experiments showed that the mechanical inoculation of solanaceous plants with PVY-Ros1 induced the formation of red infection foci in inoculated tissue and solid dark red pigmentation in systemically infected tissue, which allows disease progression to be easily monitored. By using silver nanoparticles, a nanomaterial with exciting antimicrobial properties, we proved the benefits of PVY-Ros1 to analyze novel antiviral treatments in plants. PVY-Ros1 was also helpful for visually monitoring the virus transmission process by an aphid vector. Most importantly, the anthocyanin analysis of infected tobacco tissues demonstrated that PVY-Ros1 is an excellent biotechnological tool for molecular farming because it induces the accumulation of larger amounts of anthocyanins, antioxidant compounds of nutritional, pharmaceutical and industrial interest, than those that naturally accumulate in some fruits and vegetables well known for their high anthocyanin content. Hence these results support the notion that the virus-mediated expression of regulatory factors and enzymes in plants facilitates easy quick plant metabolism engineering. es_ES
dc.description.sponsorship This research was supported by grants BIO2014-54269-R and AGL2013-49919-EXP to J-AD and AGL2013-42537-R to J-JL-M from the Ministerio de Economia y Competitividad (MINECO, co-financed FEDER funds), Spain. MM was supported by the Erasmus Mundus Scholarship-ACTION 2 WELCOME program of the European Commission. Research in CRAG is supported in part by CERCA (Generalitat de Catalunya) and by "Severo Ochoa Programme for Centres of Excellence in R&D" 2016-2019 (SEV-2015-0533). es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Microbiology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Potato virus es_ES
dc.subject Aanthocyanin es_ES
dc.subject Silver nanoparticles es_ES
dc.subject Aphid vector es_ES
dc.subject Molecular farming es_ES
dc.title A Recombinant Potato virus Y Infectious Clone Tagged with the Rosea1 Visual Marker (PVY-Ros1) Facilitates the Analysis of Viral Infectivity and Allows the Production of Large Amounts of Anthocyanins in Plants es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fmicb.2017.00611 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//SEV-2015-0533/ES/AGR-CONSORCI CSIC-IRTA-UAB CENTRE DE RECERCA EN AGRIGENOMICA (CRAG)/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2014-54269-R/ES/INSTRUMENTOS BIOTECNOLOGICOS DERIVADOS DE VIRUS DE PLANTAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2013-42537-R/ES/INFECCIONES MIXTAS DE VIRUS DE PLANTAS QUE CAUSAN ENFERMEDADES EN COSECHAS: EFECTOS SOBRE LA TRANSMISION POR VECTORES Y LA RESISTENCIA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2013-49919-EXP/ES/DETECCION DE PATOGENOS Y BIOCOMPUTACION MEDIANTE CIRCUITOS REGULADORES EN PLANTAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Cordero, T.; Mohamed, M.; Lopez Moya, J.; Daros Arnau, JA. (2017). A Recombinant Potato virus Y Infectious Clone Tagged with the Rosea1 Visual Marker (PVY-Ros1) Facilitates the Analysis of Viral Infectivity and Allows the Production of Large Amounts of Anthocyanins in Plants. Frontiers in Microbiology. 8:1-11. https://doi.org/10.3389/fmicb.2017.00611 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fmicb.2017.00611 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 11 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.identifier.pmid 28428782 es_ES
dc.identifier.pmcid PMC5382215 es_ES
dc.relation.pasarela S\356536 es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Generalitat de Catalunya es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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