- -

Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM)

Show simple item record

Files in this item

dc.contributor.author Cervera-Chiner, Lourdes es_ES
dc.contributor.author March, Carmen es_ES
dc.contributor.author Arnau Vives, Antonio es_ES
dc.contributor.author Jiménez Jiménez, Yolanda es_ES
dc.contributor.author Montoya, Ángel es_ES
dc.date.accessioned 2020-09-04T03:30:25Z
dc.date.available 2020-09-04T03:30:25Z
dc.date.issued 2020-01-21 es_ES
dc.identifier.issn 0022-5142 es_ES
dc.identifier.uri http://hdl.handle.net/10251/149414
dc.description.abstract [EN] BACKGROUND: In recent years there has been a concern about the presence of pesticides in honey because residues of DDT and carbaryl were found in honey samples. Traditional techniques, such as chromatography, reach the required limits of detection (LOD) but are not suitable for in situ implementation in the honey-packaging industry due to their high cost and the need for highly quali¿ed staff for routine operation. Biosensors offer simplicity, low cost, and easy handling for analytical purposes in food applications. RESULTS: Piezoelectric immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM) have been developed for the detection of carbaryl and DDT in honey. Biorecognition was based on competitive immunoassays in the conjugate-coated format, using monoclonal antibodies as speci¿c immunoreagents. The assay LODs attained by the HFF-QCM immunosensors were 0.05 ~gL¿1 for carbaryl and 0.24 ~gL¿1 for DDT, reaching a similar level of detectability to that of the usual reference techniques. The practical LODs in honey samples were 8 ~gkg¿1 for carbaryl and 24 ~gkg¿1 for DDT. The immunosensors¿ analytical performance allow the detection of these pesticides in honey at EU regulatory levels with good accuracy (recovery percentages ranging from 94% to 130% within the working range of each pesticide standard curve) and precision (coef¿cients of variation in the 9¿36% range). CONCLUSION: The proposed immunosensor is a promising analytical tool that could be implemented for quality control in the honey packaging industry, to simplify and to reduce the cost of the routine pesticide analysis in this appreciated natural food. es_ES
dc.description.sponsorship This research was funded by the Spanish Ministry of Economy and Competitiveness with FEDER funds (AGL Project 2013-48646-R). L.C.C. was the recipient of a PhD fellowship from the European Social Fund and the Generalitat Valenciana (ACIF / 2016/132) es_ES
dc.language Inglés es_ES
dc.publisher John Wiley & Sons es_ES
dc.relation.ispartof Journal of the Science of Food and Agriculture es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Immunosensors es_ES
dc.subject HFF-QCM es_ES
dc.subject Pesticides es_ES
dc.subject Carbaryl es_ES
dc.subject DDT es_ES
dc.subject Honey es_ES
dc.subject.classification TECNOLOGIA DE ALIMENTOS es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM) es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1002/jsfa.10267 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2013-48646-R/ES/DISEÑO DE UNA PLATAFORMA BASADA EN MATRICES DE SENSORES DE CUARZO DE ALTA RESOLUCION PARA HTS (HIGH-THROUGHPUT SCREENING) DE RESIDUOS EN MIELES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2016%2F132/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano - Institut Interuniversitari d'Investigació en Bioenginyeria i Tecnologia Orientada a l'Ésser Humà es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Tecnología de Alimentos - Departament de Tecnologia d'Aliments es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.description.bibliographicCitation Cervera-Chiner, L.; March, C.; Arnau Vives, A.; Jiménez Jiménez, Y.; Montoya, Á. (2020). Detection of DDT and carbaryl pesticides in honey by means of immunosensors based on high fundamental frequency quartz crystal microbalance (HFF-QCM). Journal of the Science of Food and Agriculture. 100(6):2468-2472. https://doi.org/10.1002/jsfa.10267 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1002/jsfa.10267 es_ES
dc.description.upvformatpinicio 2468 es_ES
dc.description.upvformatpfin 2472 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 100 es_ES
dc.description.issue 6 es_ES
dc.identifier.pmid 31965575 es_ES
dc.relation.pasarela S\407023 es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.description.references Blasco, C., Fernández, M., Pena, A., Lino, C., Silveira, M. I., Font, G., & Picó, Y. (2003). Assessment of Pesticide Residues in Honey Samples from Portugal and Spain. Journal of Agricultural and Food Chemistry, 51(27), 8132-8138. doi:10.1021/jf034870m es_ES
dc.description.references RISSATO, S., GALHIANE, M., DEALMEIDA, M., GERENUTTI, M., & APON, B. (2007). Multiresidue determination of pesticides in honey samples by gas chromatography–mass spectrometry and application in environmental contamination. Food Chemistry, 101(4), 1719-1726. doi:10.1016/j.foodchem.2005.10.034 es_ES
dc.description.references Ruiz-Toledo, J., Vandame, R., Castro-Chan, R., Penilla-Navarro, R., Gómez, J., & Sánchez, D. (2018). Organochlorine Pesticides in Honey and Pollen Samples from Managed Colonies of the Honey Bee Apis mellifera Linnaeus and the Stingless Bee Scaptotrigona mexicana Guérin from Southern, Mexico. Insects, 9(2), 54. doi:10.3390/insects9020054 es_ES
dc.description.references Juan-Borrás, M., Domenech, E., & Escriche, I. (2016). Mixture-risk-assessment of pesticide residues in retail polyfloral honey. Food Control, 67, 127-134. doi:10.1016/j.foodcont.2016.02.051 es_ES
dc.description.references Marrazza, G. (2014). Piezoelectric Biosensors for Organophosphate and Carbamate Pesticides: A Review. Biosensors, 4(3), 301-317. doi:10.3390/bios4030301 es_ES
dc.description.references March, C., García, J. V., Sánchez, Á., Arnau, A., Jiménez, Y., García, P., … Montoya, Á. (2015). High-frequency phase shift measurement greatly enhances the sensitivity of QCM immunosensors. Biosensors and Bioelectronics, 65, 1-8. doi:10.1016/j.bios.2014.10.001 es_ES
dc.description.references Cervera-Chiner, L., Juan-Borrás, M., March, C., Arnau, A., Escriche, I., Montoya, Á., & Jiménez, Y. (2018). High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCM) immunosensor for pesticide detection in honey. Food Control, 92, 1-6. doi:10.1016/j.foodcont.2018.04.026 es_ES
dc.description.references Montoya, A., March, C., Montagut, Y., Moreno, M., Manclus, J., Arnau, A., … Torres, R. (2017). A High Fundamental Frequency (HFF)-based QCM Immunosensor for Tuberculosis Detection. Current Topics in Medicinal Chemistry, 17(14), 1623-1630. doi:10.2174/1568026617666161104105210 es_ES
dc.description.references Rawson, A., Venu, S., Santhi, K., Paranthaman, R., & Sureshkumar, K. (2019). Liquid chromatography–mass spectrometry/mass spectrometry method development for the determination of carbaryl residue in honey. Pharmacognosy Magazine, 15(64), 205. doi:10.4103/pm.pm_641_18 es_ES
dc.description.references Abad, A., Primo, J., & Montoya, A. (1997). Development of an Enzyme-Linked Immunosorbent Assay to Carbaryl. 1. Antibody Production from Several Haptens and Characterization in Different Immunoassay Formats. Journal of Agricultural and Food Chemistry, 45(4), 1486-1494. doi:10.1021/jf9506904 es_ES
dc.description.references Abad, A., Manclús, J. J., Mojarrad, F., Mercader, J. V., Miranda, M. A., Primo, J., … Montoya, A. (1997). Hapten Synthesis and Production of Monoclonal Antibodies to DDT and Related Compounds. Journal of Agricultural and Food Chemistry, 45(9), 3694-3702. doi:10.1021/jf9704219 es_ES
dc.description.references González-Martínez, M. A., Morais, S., Puchades, R., Maquieira, A., Abad, A., & Montoya, A. (1997). Monoclonal Antibody-Based Flow-Through Immunosensor for Analysis of Carbaryl. Analytical Chemistry, 69(14), 2812-2818. doi:10.1021/ac961068t es_ES
dc.description.references March, C., Manclús, J. J., Jiménez, Y., Arnau, A., & Montoya, A. (2009). A piezoelectric immunosensor for the determination of pesticide residues and metabolites in fruit juices. Talanta, 78(3), 827-833. doi:10.1016/j.talanta.2008.12.058 es_ES
dc.description.references Mauriz, E., Calle, A., Manclús, J. J., Montoya, A., Hildebrandt, A., Barceló, D., & Lechuga, L. M. (2007). Optical immunosensor for fast and sensitive detection of DDT and related compounds in river water samples. Biosensors and Bioelectronics, 22(7), 1410-1418. doi:10.1016/j.bios.2006.06.016 es_ES
dc.description.references Sapozhnikova, Y., Simons, T., & Lehotay, S. J. (2015). Evaluation of a Fast and Simple Sample Preparation Method for Polybrominated Diphenyl Ether (PBDE) Flame Retardants and Dichlorodiphenyltrichloroethane (DDT) Pesticides in Fish for Analysis by ELISA Compared with GC-MS/MS. Journal of Agricultural and Food Chemistry, 63(18), 4429-4434. doi:10.1021/jf505651g es_ES
dc.description.references Sun, J., Dong, T., Zhang, Y., & Wang, S. (2010). Development of enzyme linked immunoassay for the simultaneous detection of carbaryl and metolcarb in different agricultural products. Analytica Chimica Acta, 666(1-2), 76-82. doi:10.1016/j.aca.2010.03.051 es_ES
dc.description.references Mauriz, E., Calle, A., Abad, A., Montoya, A., Hildebrandt, A., Barceló, D., & Lechuga, L. M. (2006). Determination of carbaryl in natural water samples by a surface plasmon resonance flow-through immunosensor. Biosensors and Bioelectronics, 21(11), 2129-2136. doi:10.1016/j.bios.2005.10.013 es_ES


This item appears in the following Collection(s)

Show simple item record