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Characterization of the efficiency and uncertainty of skimmed milk flocculation for the simultaneous concentration and quantification of water-borne viruses, bacteria and protozoa

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Characterization of the efficiency and uncertainty of skimmed milk flocculation for the simultaneous concentration and quantification of water-borne viruses, bacteria and protozoa

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dc.contributor.author Gonzales-Gustavson, Eloy es_ES
dc.contributor.author Cárdenas-Youngs, Yexenia es_ES
dc.contributor.author Calvo, Miquel es_ES
dc.contributor.author Figueira, Marcelle es_ES
dc.contributor.author Hundesa, Ayalkibet es_ES
dc.contributor.author Amoros, Inmaculada es_ES
dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.contributor.author Moreno-Mesonero, Laura es_ES
dc.contributor.author Rosell, Rosa es_ES
dc.contributor.author Ganges, Llilianne es_ES
dc.contributor.author Araujo, R. es_ES
dc.contributor.author Gironés, R. es_ES
dc.date.accessioned 2020-09-08T03:31:49Z
dc.date.available 2020-09-08T03:31:49Z
dc.date.issued 2017-03 es_ES
dc.identifier.issn 0167-7012 es_ES
dc.identifier.uri http://hdl.handle.net/10251/149536
dc.description.abstract [EN] In this study, the use of skimmed milk flocculation (SMF) to simultaneously concentrate viruses, bacteria and protozoa was evaluated. We selected strains of faecal indicator bacteria and pathogens, such as Escherichia coli and Helicobacter pylori. The viruses selected were adenovirus (HAdV 35), rotavirus (RoV SA-11), the bacteriophage MS2 and bovine viral diarrhoea virus (BVDV). The protozoa tested were Acanthamoeba, Giardia and cryptosporidium. The mean recoveries with q(RT)PCR were 66% (HAdV 35), 24% (MS2), 28% (RoV SA-11), 15% (BVDV), 60% (E. coli), 30% (H. pylori) and 21% (Acanthamoeba castellanii). When testing the infectivity, the mean recoveries were 59% (HAdV 35), 12% (MS2), 26% (RoV SA-11) and 0.7% (BVDV). The protozoa Giardia lamblia and Cryptosporidium parvum were studied by immunofluorescence with recoveries of 18% and 13%, respectively. Although q(RT)PCR consistently showed higher quantification values (as expected), q(RT)PCR and the infectivity assays showed similar recoveries for HAdV 35 and RoV SA-11. Additionally, we investigated modelling the variability and uncertainty of the recovery with this method to extrapolate the quantification obtained by q(RT)PCR and estimate the real concentration. The 95% prediction intervals of the real concentration of the microorganisms inoculated were calculated using a general non-parametric bootstrap procedure adapted in our context to estimate the technical error of the measurements. SMF shows recoveries with a low variability that permits the use of a mathematical approximation to predict the concentration of the pathogen and indicator with acceptable low intervals. The values of uncertainty may be used for a quantitative microbial risk analysis or diagnostic purposes. (C) 2017 The Author(s). Published by Elsevier B.V. es_ES
dc.description.sponsorship This study was funded by the Water Challenges for a Changing World Joint Programming Initiative (W2013-095-C03-01), the Spanish Ministry of Economy and Competitiveness (MINECO - AGL2014-55081-R) and the Grup de Recerca Consolidat: Virus, bacteris i protozous d'interes en salut publica, aigua y aliments (Generalitat de Catalunya, Virbap - 2014-SGR-914). Eloy Gonzales-Gustavson would like to acknowledge the Presidente de la República scholarship from the Peruvian Government, which supported a PhD grant. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//AGL2014-55081-R/ES/DETECCION SENSIBLE DE VIRUS TRANSMITIDOS POR ALIMENTOS UTILIZANDO AMPLIFICACION DIRIGIDA Y NGS Y EVALUACION DE LA TRANSMISION Y PATOLOGIA DE LOS VIRUS DNA EXCRETADOS/ es_ES
dc.relation GC/2014-SGR-914 es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//JPIW2013-095-C03-02/ES/NUEVOS INSTRUMENTOS MOLECULARES Y DE METAGENOMICA PARA LA IDENTIFICACION Y CONTROL A ESCALA EUROPEA DE CONTAMINANTES MICROBIANOS EMERGENTES EN AGUA DE RIEGO/ es_ES
dc.relation.ispartof Journal of Microbiological Methods es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Skimmed milk flocculation es_ES
dc.subject Water contamination es_ES
dc.subject Microorganisms es_ES
dc.subject Q(RT)PCR es_ES
dc.subject Recovery es_ES
dc.subject Real concentration es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Characterization of the efficiency and uncertainty of skimmed milk flocculation for the simultaneous concentration and quantification of water-borne viruses, bacteria and protozoa es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.mimet.2017.01.006 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.description.bibliographicCitation Gonzales-Gustavson, E.; Cárdenas-Youngs, Y.; Calvo, M.; Figueira, M.; Hundesa, A.; Amoros, I.; Moreno Trigos, MY.... (2017). Characterization of the efficiency and uncertainty of skimmed milk flocculation for the simultaneous concentration and quantification of water-borne viruses, bacteria and protozoa. Journal of Microbiological Methods. 134:46-53. https://doi.org/10.1016/j.mimet.2017.01.006 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.mimet.2017.01.006 es_ES
dc.description.upvformatpinicio 46 es_ES
dc.description.upvformatpfin 53 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 134 es_ES
dc.identifier.pmid 28093213 es_ES
dc.relation.pasarela S\342210 es_ES
dc.contributor.funder Generalitat de Catalunya es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES


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