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Different rates of spontaneous mutation of chloroplastic and nuclear viroids as determined by high-fidelity ultra-deep sequencing

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Different rates of spontaneous mutation of chloroplastic and nuclear viroids as determined by high-fidelity ultra-deep sequencing

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dc.contributor.author López-Carrasco, María Amparo es_ES
dc.contributor.author Ballesteros Martínez, Cristina es_ES
dc.contributor.author Sentandreu, V. es_ES
dc.contributor.author Delgado Villar, Sonia Guadalupe es_ES
dc.contributor.author Gago Zachert, Selma Persida es_ES
dc.contributor.author FLORES PEDAUYE, RICARDO es_ES
dc.contributor.author Sanjuan Verdeguer, Rafael es_ES
dc.date.accessioned 2020-09-10T03:31:41Z
dc.date.available 2020-09-10T03:31:41Z
dc.date.issued 2017-09-14 es_ES
dc.identifier.issn 1553-7366 es_ES
dc.identifier.uri http://hdl.handle.net/10251/149717
dc.description.abstract [EN] Mutation rates vary by orders of magnitude across biological systems, being higher for simpler genomes. The simplest known genomes correspond to viroids, subviral plant replicons constituted by circular non-coding RNAs of few hundred bases. Previous work has revealed an extremely high mutation rate for chrysanthemum chlorotic mottle viroid, a chloroplastreplicating viroid. However, whether this is a general feature of viroids remains unclear. Here, we have used high-fidelity ultra-deep sequencing to determine the mutation rate in a common host (eggplant) of two viroids, each representative of one family: the chloroplastic eggplant latent viroid (ELVd, Avsunviroidae) and the nuclear potato spindle tuber viroid (PSTVd, Pospiviroidae). This revealed higher mutation frequencies in ELVd than in PSTVd, as well as marked differences in the types of mutations produced. Rates of spontaneous mutation, quantified in vivo using the lethal mutation method, ranged from 1/1000 to 1/800 for ELVd and from 1/7000 to 1/3800 for PSTVd depending on sequencing run. These results suggest that extremely high mutability is a common feature of chloroplastic viroids, whereas the mutation rates of PSTVd and potentially other nuclear viroids appear significantly lower and closer to those of some RNA viruses. es_ES
dc.description.sponsorship This work was supported by the European Research Council (erc.europa.eu; ERC-2011-StG-281191-VIRMUT to RS), the Spanish Ministerio de Economia y Competitividad (www.mineco.gob.es; BFU2013-41329 grant to RS, BFU2014-56812-P grant to RF, and a predoctoral fellowship to ALC), and the Spanish Junta de Comunidades de Castilla-La Mancha (www.castillalamancha.es;postdoctoral fellowship to CB). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. es_ES
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS Pathogens es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject RNA-Polymerase es_ES
dc.subject Self-Cleavage es_ES
dc.subject In-Vivo es_ES
dc.subject Replication es_ES
dc.subject Motif es_ES
dc.subject Mutagenesis es_ES
dc.subject Generation es_ES
dc.subject Evolution es_ES
dc.subject Ribozyme es_ES
dc.subject Matter es_ES
dc.title Different rates of spontaneous mutation of chloroplastic and nuclear viroids as determined by high-fidelity ultra-deep sequencing es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.ppat.1006547 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/281191/EU/Variability in the mutation rate of RNA viruses/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2013-41329-P/ES/REGULACION DE LA TASA DE MUTACION EN VIRUS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BFU2014-56812-P/ES/VIROIDES, LOS PARASITOS EXTREMOS: EVOLUCION ESPACIO-TEMPORAL, PATOGENESIS MEDIADA POR SILENCIAMIENTO VIA RNA, Y DEGRADACION/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation López-Carrasco, MA.; Ballesteros Martínez, C.; Sentandreu, V.; Delgado Villar, SG.; Gago Zachert, SP.; Flores Pedauye, R.; Sanjuan Verdeguer, R. (2017). Different rates of spontaneous mutation of chloroplastic and nuclear viroids as determined by high-fidelity ultra-deep sequencing. PLoS Pathogens. 13(9):1-17. https://doi.org/10.1371/journal.ppat.1006547 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1371/journal.ppat.1006547 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.description.issue 9 es_ES
dc.identifier.pmid 28910391 es_ES
dc.identifier.pmcid PMC5614642 es_ES
dc.relation.pasarela S\357596 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Junta de Comunidades de Castilla-La Mancha es_ES
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