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Metagenomic analysis of viruses, bacteria and protozoa in irrigation water

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Metagenomic analysis of viruses, bacteria and protozoa in irrigation water

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dc.contributor.author Rusiñol, Marta es_ES
dc.contributor.author Martinez-Puchol, Sandra es_ES
dc.contributor.author Timoneda, Natalia es_ES
dc.contributor.author Fernandez-Cassi, Xavier es_ES
dc.contributor.author Pérez-Cataluña, A. es_ES
dc.contributor.author Fernández-Bravo, A. es_ES
dc.contributor.author Moreno-Mesonero, Laura es_ES
dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.contributor.author Alonso Molina, José Luís es_ES
dc.contributor.author Figueras, M.J. es_ES
dc.contributor.author Abril, J.F. es_ES
dc.contributor.author Bofill-Mas, S. es_ES
dc.contributor.author Gironés, R. es_ES
dc.date.accessioned 2020-09-24T03:33:41Z
dc.date.available 2020-09-24T03:33:41Z
dc.date.issued 2020-03 es_ES
dc.identifier.issn 1438-4639 es_ES
dc.identifier.uri http://hdl.handle.net/10251/150594
dc.description.abstract [EN] Viruses (e.g., noroviruses and hepatitis A and E virus), bacteria (e.g., Salmonella spp. and pathogenic Escherichia coli) and protozoa (e.g., Cryptosporidium parvum and Giardia intestinalis) are well-known contributors to food-borne illnesses linked to contaminated fresh produce. As agricultural irrigation increases the total amount of water used annually, reclaimed water is a good alternative to reduce dependency on conventional irrigation water sources. European guidelines have established acceptable concentrations of certain pathogens and/or indicators in irrigation water, depending on the irrigation system used and the irrigated crop. However, the incidences of food-borne infections are known to be underestimated and all the different pathogens contributing to these infections are not known. Next-generation sequencing (NGS) enables the determination of the viral, bacterial and protozoan populations present in a water sample, providing an opportunity to detect emerging pathogens and develop improved tools for monitoring the quality of irrigation water. This is a descriptive study of the virome, bacteriome and parasitome present in different irrigation water sources. We applied the same concentration method for all the studied samples and specific metagenomic approaches to characterize both DNA and RNA viruses, bacteria and protozoa. In general, most of the known viral species corresponded to plant viruses and bacteriophages. Viral diversity in river water varied over the year, with higher bacteriophage prevalences during the autumn and winter. Reservoir water contained Enterobacter cloacae, an opportunistic human pathogen and an indicator of fecal contamination, as well as Naegleria australiensis and Naegleria clarki. Hepatitis E virus and Naegleria fowleri, emerging human pathogens, were detected in groundwater. Reclaimed water produced in a constructed wetland system presented a virome and bacteriome that resembled those of freshwater samples (river and reservoir water). Viral, bacterial and protozoan pathogens were occasionally detected in the different irrigation water sources included in this study, justifying the use of improved NGS techniques to get a comprehensive evaluation of microbial species and potential environmental health hazards associated to irrigation water. es_ES
dc.description.sponsorship This work was supported through a grant funded by the Spanish Ministry of Economy and Competitiveness (MINECO) in the frame of the collaborative international consortium JPIW2013-095-C03-01, JPIW2013-095-C03-02 and JPIW2013-095-C03-03 of the Water Challenges for a Changing World Joint Programming Initiative (Water JPI) Pilot Call and partially by AGL2017-86797-C2-1-R. Silvia Bofill-Mas is a Serra-Hunter fellow at the University of Barcelona. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//JPIW2013-095-C03-01/ES/NUEVOS INSTRUMENTOS MOLECULARES Y DE METAGENOMICA PARA LA IDENTIFICACION Y CONTROL A ESCALA EUROPEA DE CONTAMINANTES MICROBIANOS EMERGENTES EN AGUA DE RIEGO/ es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//JPIW2013-095-C03-03/ES/NUEVOS INSTRUMENTOS MOLECULARES Y DE METAGENOMICA PARA LA IDENTIFICACION Y CONTROL A ESCALA EUROPEA DE CONTAMINANTES MICROBIANOS EMERGENTES EN AGUA DE RIEGO/ es_ES
dc.relation MINECO/AGL2017-86797-C2-1-R es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//JPIW2013-095-C03-02/ES/NUEVOS INSTRUMENTOS MOLECULARES Y DE METAGENOMICA PARA LA IDENTIFICACION Y CONTROL A ESCALA EUROPEA DE CONTAMINANTES MICROBIANOS EMERGENTES EN AGUA DE RIEGO/ es_ES
dc.relation.ispartof International Journal of Hygiene and Environmental Health es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Irrigation water es_ES
dc.subject Metagenomics es_ES
dc.subject Virus es_ES
dc.subject Bacteria es_ES
dc.subject Protozoa es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Metagenomic analysis of viruses, bacteria and protozoa in irrigation water es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.ijheh.2019.113440 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.description.bibliographicCitation Rusiñol, M.; Martinez-Puchol, S.; Timoneda, N.; Fernandez-Cassi, X.; Pérez-Cataluña, A.; Fernández-Bravo, A.; Moreno-Mesonero, L.... (2020). Metagenomic analysis of viruses, bacteria and protozoa in irrigation water. International Journal of Hygiene and Environmental Health. 224. https://doi.org/10.1016/j.ijheh.2019.113440 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.ijheh.2019.113440 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 224 es_ES
dc.identifier.pmid 31978735 es_ES
dc.relation.pasarela S\406760 es_ES
dc.contributor.funder Ministerio de Economía y Empresa es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES


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