Mostrar el registro sencillo del ítem
dc.contributor.author | Kulikova, Barbora | es_ES |
dc.contributor.author | Jiménez-Trigos, María Estrella | es_ES |
dc.contributor.author | Makarevich, Alexander V. | es_ES |
dc.contributor.author | Chrenek, Peter | es_ES |
dc.contributor.author | Vicente Antón, José Salvador | es_ES |
dc.contributor.author | Marco-Jiménez, Francisco | es_ES |
dc.date.accessioned | 2020-09-24T12:28:02Z | |
dc.date.available | 2020-09-24T12:28:02Z | |
dc.date.issued | 2016-02 | es_ES |
dc.identifier.issn | 0011-2240 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/150611 | |
dc.description.abstract | [EN] This study was focused on the effect of cryopreservation on the state of actin cytoskeleton and development of rabbit pronuclear zygotes. Zygotes were collected from superovulated females and immediately used for 1) slow-freezing in a solution containing 1.5 M 1,2-propanediol and 0.2 M sucrose, or 2) vitrification in a solution containing 42.0% (v/v) of ethylene glycol, 18.0% (w/v) of dextran and 0.3 M sucrose as cryoprotectants. After thawing or warming, respectively, zygotes were evaluated for 1) actin distribution, 2) in vitro or 3) in vivo development to blastocyst. Comparing actin filaments distribution, a significantly higher number of vitrified zygotes with actin distributed in cell border was observed (55 ± 7.7 vs. 74 ± 6.1% for slow-frozen vs. vitrified, respectively). After 24 and 72 h of in vitro development, significant differences in the cleavage and morula rate among the groups were observed (9 ± 2.4 and 3 ± 1.3 vs. 44 ± 3.0 and 28 ± 2.7% for slow-frozen vs. vitrified, respectively). None of the slow-frozen zygotes reached the blastocyst stage, in contrast to the vitrified counterparts (11 ± 1.9%). Under in vivo culture conditions, a significant difference in blastocyst rate was observed between vitrified and fresh embryos (6 ± 1.5 vs. 35 ± 4.4% respectively). Our results showed that alterations in actin cytoskeleton and deteriorated development are more evident in slow-frozen than vitrified pronuclear zygotes. Vitrification method seems to be a more effective option for rabbit zygotes cryopreservation, although pronuclear zygotes manipulation per se resulted in a notable decrease in embryo development. | es_ES |
dc.description.sponsorship | This research was supported by the projects: UGAVIII/16/2015, VEGA 1/0611/15, by the Spanish Research project AGL2014-53405-C2-1-PComision Interministerial de Ciencia y Tecnologia (CICYT), Generalitat Valenciana research program (Prometeo II 2014/036), grant of Slovak Research and Development Agency: APVV-14-0043 and by the European Community under project no 26220220180: Building Research Centre "AgroBioTech". B. Kulikova received fellowship from a Collaborative European Network on Rabbit Genome Biology (RGB-Net) (COST-STSM-TD1101) | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Cryobiology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Pronuclear | es_ES |
dc.subject | Slow-freezing | es_ES |
dc.subject | Vitrification | es_ES |
dc.subject | Actin filaments | es_ES |
dc.subject | Development | es_ES |
dc.subject | Rabbit | es_ES |
dc.subject.classification | PRODUCCION ANIMAL | es_ES |
dc.title | State of actin cytoskeleton and development of slow-frozen and vitrified rabbit pronuclear zygotes | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.cryobiol.2015.11.009 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/SRDA//APVV-14-0043/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Ministry of Education, Science, Research and Sport of the Slovak Republic//26220220180/SK/Building Research Centre “AgroBioTech“/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/COST//TD1101/EU/A Collaborative European Network on Rabbit Genome Biology (RGB-Net)/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F036/ES/Crioconservación de óvulos y embriones. Cambios epigenéticos y repercusiones transcripcionales y proteómicas/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2014-53405-C2-1-P/ES/MEJORA GENETICA DEL CONEJO DE CARNE:RESPUESTA A LA SELECCION Y SU EFECTO SOBRE LA REPRODUCCION, ALIMENTACION Y SALUD UTILIZANDO UNA POBLACION CONTROL CRIOCONSERVADA/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal | es_ES |
dc.description.bibliographicCitation | Kulikova, B.; Jiménez-Trigos, ME.; Makarevich, AV.; Chrenek, P.; Vicente Antón, JS.; Marco-Jiménez, F. (2016). State of actin cytoskeleton and development of slow-frozen and vitrified rabbit pronuclear zygotes. Cryobiology. 72(1):14-20. https://doi.org/10.1016/j.cryobiol.2015.11.009 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.cryobiol.2015.11.009 | es_ES |
dc.description.upvformatpinicio | 14 | es_ES |
dc.description.upvformatpfin | 20 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 72 | es_ES |
dc.description.issue | 1 | es_ES |
dc.identifier.pmid | 26687386 | es_ES |
dc.relation.pasarela | S\300823 | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | European Cooperation in Science and Technology | es_ES |
dc.contributor.funder | Slovak Research and Development Agency | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Economía, Industria y Competitividad | es_ES |
dc.contributor.funder | Collaborative European Network on Rabbit Genome Biology | es_ES |