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Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast

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Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast

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dc.contributor.author Vanacloig-Pedrós, Elena es_ES
dc.contributor.author Proft, Markus Hans es_ES
dc.contributor.author Pascual-Ahuir Giner, María Desamparados es_ES
dc.date.accessioned 2020-09-24T12:28:51Z
dc.date.available 2020-09-24T12:28:51Z
dc.date.issued 2016-09-22 es_ES
dc.identifier.uri http://hdl.handle.net/10251/150635
dc.description.abstract [EN] Citrinin (CIT) and ochratoxin A (OTA) are important mycotoxins, which frequently co-contaminate foodstuff. In order to assess the toxicologic threat posed by the two mycotoxins separately or in combination, their biological effects were studied here using genomic transcription profiling and specific live cell gene expression reporters in yeast cells. Both CIT and OTA cause highly transient transcriptional activation of different stress genes, which is greatly enhanced by the disruption of the multidrug exporter Pdr5. Therefore, we performed genome-wide transcription profiling experiments with the pdr5 mutant in response to acute CIT, OTA, or combined CIT/OTA exposure. We found that CIT and OTA activate divergent and largely nonoverlapping gene sets in yeast. CIT mainly caused the rapid induction of antioxidant and drug extrusion-related gene functions, while OTA mainly deregulated developmental genes related with yeast sporulation and sexual reproduction, having only a minor effect on the antioxidant response. The simultaneous exposure to CIT and OTA gave rise to a genomic response, which combined the specific features of the separated mycotoxin treatments. The application of stress-specific mutants and reporter gene fusions further confirmed that both mycotoxins have divergent biological effects in cells. Our results indicate that CIT exposure causes a strong oxidative stress, which triggers a massive transcriptional antioxidant and drug extrusion response, while OTA mainly deregulates developmental genes and only marginally induces the antioxidant defense. es_ES
dc.description.sponsorship We thank Lorena Latorre and Javier Forment for their help with the microarray experiments and data analysis. This work was funded only in the initial phase by a grant from Ministerio de Economía y Competitividad (BFU2011-23326). We thank the Fond for Open Access Publication from Consejo Superior de Investigaciones Científicas (CSIC) for supporting publication costs of this article. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Toxins es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Ochratoxin A es_ES
dc.subject Citrinin es_ES
dc.subject Transcriptome es_ES
dc.subject Saccharomyces cerevisiae es_ES
dc.subject Mycotoxins es_ES
dc.subject Oxidative stress es_ES
dc.subject Dose response es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/toxins8100273 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BFU2011-23326/ES/REGULACION DE LA CROMATINA Y DE LA ESTRUCTURA MITOCONDRIAL EN RESPUESTA A ESTRES OSMOTICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Vanacloig-Pedrós, E.; Proft, MH.; Pascual-Ahuir Giner, MD. (2016). Different Toxicity Mechanisms for Citrinin and Ochratoxin A Revealed by Transcriptomic Analysis in Yeast. Toxins. 8(10):1-20. https://doi.org/10.3390/toxins8100273 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/toxins8100273 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 20 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 8 es_ES
dc.description.issue 10 es_ES
dc.identifier.eissn 2072-6651 es_ES
dc.identifier.pmid 27669300 es_ES
dc.identifier.pmcid PMC5086634 es_ES
dc.relation.pasarela S\337379 es_ES
dc.contributor.funder Ministerio de Ciencia, Innovación y Universidades es_ES
dc.contributor.funder Consejo Superior de Investigaciones Científicas es_ES
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