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Expression of two barley proteinase inhibitors in tomato promotes endogenous defensive response and enhances resistance to Tuta absoluta

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Expression of two barley proteinase inhibitors in tomato promotes endogenous defensive response and enhances resistance to Tuta absoluta

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dc.contributor.author Hamza, Rim es_ES
dc.contributor.author Pérez-Hedo, Meritxell es_ES
dc.contributor.author Urbaneja, Alberto es_ES
dc.contributor.author Rambla Nebot, Jose Luis es_ES
dc.contributor.author GRANELL RICHART, ANTONIO es_ES
dc.contributor.author Gaddour, Kamel es_ES
dc.contributor.author BELTRAN PORTER, JOSE PIO es_ES
dc.contributor.author Cañas Clemente, Luís Antonio es_ES
dc.date.accessioned 2020-10-05T06:46:59Z
dc.date.available 2020-10-05T06:46:59Z
dc.date.issued 2018-01-25 es_ES
dc.identifier.issn 1471-2229 es_ES
dc.identifier.uri http://hdl.handle.net/10251/151078
dc.description.abstract [EN] Background: For as long as 350 million years, plants and insects have coexisted and developed a set of relationships which affect both organisms at different levels. Plants have evolved various morphological and biochemical adaptations to cope with herbivores attacks. However, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) has become the major pest threatening tomato crops worldwide and without the appropriated management it can cause production losses between 80 to 100%. Results: The aim of this study was to investigate the in vivo effect of a serine proteinase inhibitor (BTI-CMe) and a cysteine proteinase inhibitor (Hv-CPI2) from barley on this insect and to examine the effect their expression has on tomato defensive response. We found that larvae fed on the double transgenic plants showed a notable reduction in weight. Moreover, only 56% of the larvae reached the adult stage. The emerged adults showed wings deformities and reduced fertility. We also investigated the effect of proteinase inhibitors ingestion on the insect digestive enzymes. Our results showed a decrease in larval trypsin activity. Transgenes expression had no harmful effect on Nesidiocoris tenuis (Reuter) (Heteroptera: Miridae), a predator of Tuta absoluta, despite transgenic tomato plants attracted the mirid. We also found that barley cystatin expression promoted plant defense by inducing the expression of the tomato endogenous wound inducible Proteinase inhibitor 2 (Pin2) gene, increasing the production of glandular trichomes and altering the emission of volatile organic compounds. Conclusion: Our results demonstrate the usefulness of the co-expression of different proteinase inhibitors for the enhancement of plant resistance to Tuta absoluta. es_ES
dc.description.sponsorship This work was partly supported by grants BIO2013-40747-R and AGL2014-55616-C3 from the Spanish Ministry of Economy and Competitiveness (MINECO) es_ES
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Proteinase inhibitors es_ES
dc.subject Tuta absoluta es_ES
dc.subject Enhanced resistance es_ES
dc.subject Induced plant defense es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Expression of two barley proteinase inhibitors in tomato promotes endogenous defensive response and enhances resistance to Tuta absoluta es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12870-018-1240-6 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2013-40747-R/ES/CARACTERIZACION DE NUEVOS GENES QUE CONTROLAN LA FRUCTIFICACION EN TOMATE: DESARROLLO DE APLICACIONES BIOTECNOLOGICAS ENFOCADAS A LA MEJORA./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2014-55616-C3-1-R/ES/MEJORA DE LA RESILIENCIA DEL CULTIVO MEDIANTE EL AUMENTO DE LA RESPUESTA DE DEFENSA DE LA PLANTA Y ADAPTACION AL CAMBIO CLIMATICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Hamza, R.; Pérez-Hedo, M.; Urbaneja, A.; Rambla Nebot, JL.; Granell Richart, A.; Gaddour, K.; Beltran Porter, JP.... (2018). Expression of two barley proteinase inhibitors in tomato promotes endogenous defensive response and enhances resistance to Tuta absoluta. BMC Plant Biology. 18. https://doi.org/10.1186/s12870-018-1240-6 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1186/s12870-018-1240-6 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 18 es_ES
dc.identifier.pmid 29370757 es_ES
dc.identifier.pmcid PMC5785808 es_ES
dc.relation.pasarela S\341243 es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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