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New insight into the SSC8 genetic determination of fatty acid composition in pigs

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New insight into the SSC8 genetic determination of fatty acid composition in pigs

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dc.contributor.author Revilla, M. es_ES
dc.contributor.author Ramayo-Caldas, Y. es_ES
dc.contributor.author Castelló, A. es_ES
dc.contributor.author Corominas, J. es_ES
dc.contributor.author Puig-Oliveras, A. es_ES
dc.contributor.author Ibañez Escriche, Noelia es_ES
dc.contributor.author Muñoz, M. es_ES
dc.contributor.author Ballester, M. es_ES
dc.contributor.author Folch, J.M. es_ES
dc.date.accessioned 2020-10-07T03:33:37Z
dc.date.available 2020-10-07T03:33:37Z
dc.date.issued 2014-04-23 es_ES
dc.identifier.issn 0999-193X es_ES
dc.identifier.uri http://hdl.handle.net/10251/151280
dc.description.abstract [EN] Background:Fat content and fatty acid composition in swine are becoming increasingly studied because of their effect on sensory and nutritional quality of meat. A QTL (quantitative trait locus) for fatty acid composition in backfat was previously detected on porcine chromosome 8 (SSC8) in an Iberian x Landrace F-2 intercross. More recently, a genome-wide association study detected the same genomic region for muscle fatty acid composition in an Iberian x Landrace backcross population. ELOVL6, a strong positional candidate gene for this QTL, contains a polymorphism in its promoter region (ELOVL6:c.-533C < T), which is associated with percentage of palmitic and palmitoleic acids in muscle and adipose tissues. Here, a combination of single-marker association and the haplotype-based approach was used to analyze backfat fatty acid composition in 470 animals of an Iberian x Landrace F2 intercross genotyped with 144 SNPs (single nucleotide polymorphisms) distributed along SSC8. Results:Two trait-associated SNP regions were identified at 93 Mb and 119 Mb on SSC8. The strongest statistical signals of both regions were observed for palmitoleic acid (C16:1(n-7)) content and C18:0/C16:0 and C18:1(n-7)/C16:1 (n-7) elongation ratios. MAML3 and SETD7 are positional candidate genes in the 93 Mb region and two novel microsatellites in MAML3 and nine SNPs in SETD7 were identified. No significant association for the MAML3 microsatellite genotypes was detected. The SETD7:c. 700G > T SNP, although statistically significant, was not the strongest signal in this region. In addition, the expression of MAML3 and SETD7 in liver and adipose tissue varied among animals, but no association was detected with the polymorphisms in these genes. In the 119 Mb region, the ELOVL6:c.-533C > T polymorphism showed a strong association with percentage of palmitic and palmitoleic fatty acids and elongation ratios in backfat. Conclusions:Our results suggest that the polymorphisms studied in MAML3 and SETD7 are not the causal mutations for the QTL in the 93 Mb region. However, the results for ELOVL6 support the hypothesis that the ELOVL6:c.-533C > T polymorphism has a pleiotropic effect on backfat and intramuscular fatty acid composition and that it has a role in the determination of the QTL in the 119 Mb region. es_ES
dc.description.sponsorship This work was funded by MICINN AGL2008-04818-C03/GAN and MINECO AGL2011-29821-C02 and the Innovation Programme Consolider-Ingenio 2010 (CSD2007-00036). M. Revilla is a Master's student of Animal Breeding and Biotechnology of Reproduction (Polytechnical University of Valencia and Autonomous University of Barcelona). Y. Ramayo-Caldas was funded by a FPU grant (AP2008-01450), J. Corominas by a FPI scholarship from the Ministry of Education (BES-2009-018223) and A. Puig-Oliveras by a PIF scholarship (458-01-1/2011). This manuscript has been proofread by Chuck Simons, a native English speaking university instructor in English. es_ES
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation.ispartof Genetics Selection Evolution es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title New insight into the SSC8 genetic determination of fatty acid composition in pigs es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/1297-9686-46-28 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2008-04818-C03-03/ES/GENES CANDIDATOS E IDENTIFICACION GENOMICA DE LOCI Y RUTAS GENICAS QUE AFECTAN A LA CALIDAD DE LA CARNE EN CERDOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2008-04818-C03-02/ES/GENES CANDIDATOS E IDENTIFICACION GENOMICA DE LOCI Y RUTAS GENETICAS QUE AFECTAN A LA CALIDAD DE LA CARNE EN CERDOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MECD//AP2008-01450/ES/AP2008-01450/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2008-04818-C03-01/ES/GENES CANDIDATOS E IDENTIFICACION GENOMICA DE LOCI Y RUTAS GENETICAS QUE AFECTAN A LA CALIDAD DE LA CARNE EN CERDOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//CSD2007-00036/ES/Centro de Genómica Básica y de orientación Agroalimentaria/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2011-29821-C02-02/ES/APLICACION DE METODOS DE SECUENCIACION PARALELA MASIVA Y GENOMICA AL ESTUDIO DE VARIANTES GENICAS QUE REGULAN:CRECIMIENTO,CONFORMACION Y CALIDAD DE CARNE EN CERDO.SUBPROYECTO2/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BES-2009-018223-2/ES/BES-2009-018223-2/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Revilla, M.; Ramayo-Caldas, Y.; Castelló, A.; Corominas, J.; Puig-Oliveras, A.; Ibañez Escriche, N.; Muñoz, M.... (2014). New insight into the SSC8 genetic determination of fatty acid composition in pigs. Genetics Selection Evolution. 46. https://doi.org/10.1186/1297-9686-46-28 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1186/1297-9686-46-28 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 46 es_ES
dc.identifier.pmid 24758572 es_ES
dc.relation.pasarela S\343532 es_ES
dc.contributor.funder Ministerio de Educación es_ES
dc.contributor.funder Universitat Autònoma de Barcelona es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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