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PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: in vitro cell culture studies

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PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: in vitro cell culture studies

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dc.contributor.author Milovac, Dajana es_ES
dc.contributor.author Gamboa-Martinez, Tatiana C. es_ES
dc.contributor.author Ivankovic, Marica es_ES
dc.contributor.author Gallego-Ferrer, Gloria es_ES
dc.contributor.author Ivankovic, Hrvoje es_ES
dc.date.accessioned 2020-10-07T03:34:58Z
dc.date.available 2020-10-07T03:34:58Z
dc.date.issued 2014-09-01 es_ES
dc.identifier.issn 0928-4931 es_ES
dc.identifier.uri http://hdl.handle.net/10251/151306
dc.description.abstract [EN] In the present study, we examined the potential of using highly porous poly(epsilon-caprolactone) (PCL)-coated hydroxyapatite (HAp) scaffold derived from cuttlefish bone for bone tissue engineering applications. The cell culture studies were performed in vitro with preosteoblastic MC3T3-E1 cells in static culture conditions. Comparisons were made with uncoated HAp scaffold. The attachment and spreading of preosteoblasts on scaffolds were observed by Live/Dead staining Kit. The cells grown on the HAp/PCL composite scaffold exhibited greater spreading than cells grown on the HAp scaffold. DNA quantification and scanning electron microscopy (SEM) confirmed a good proliferation of cells on the scaffolds. DNA content on the HAp/PCL scaffold was significantly higher compared to porous HAp scaffolds. The amount of collagen synthesis was determined using a hydroxyproline assay. The osteoblastic differentiation of the cells was evaluated by determining alkaline phosphatase (ALP) activity and collagen type I secretion. Furthermore, cell spreading and cell proliferation within scaffolds were observed using a fluorescence microscope. (C) 2014 Elsevier B.V. All rights reserved. es_ES
dc.description.sponsorship The financial support of the Ministry of Science, Education and Sports of the Republic of Croatia (Project 125-1252970-3005: "Bioceramic, Polymer and Composite Nanostructured Materials"), the Spanish Ministry project DPI2010-20399-C04-03 and the L'Oreal ADRIA-UNESCO national fellowship program for Women in Science is gratefully acknowledged. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Materials Science and Engineering C es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cuttlefish bone es_ES
dc.subject Poly(epsilon-caprolactone) es_ES
dc.subject Hydroxyapatite scaffold es_ES
dc.subject Tissue engineering es_ES
dc.subject Cell differentiation es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: in vitro cell culture studies es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.msec.2014.05.034 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MZOS//125-1252970-3005/HR/Bioceramic, Polymer and Composite Nanostructured Materials/
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//DPI2010-20399-C04-03/ES/DISEÑO Y FABRICACION DE UNA PLATAFORMA BIOMIMETICA TIPO SCAFFOLD%2FSOPORTE PARA LA REGENERACION DEL CARTILAGO ARTICULAR/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Milovac, D.; Gamboa-Martinez, TC.; Ivankovic, M.; Gallego-Ferrer, G.; Ivankovic, H. (2014). PCL-coated hydroxyapatite scaffold derived from cuttlefish bone: in vitro cell culture studies. Materials Science and Engineering C. 42:264-272. https://doi.org/10.1016/j.msec.2014.05.034 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.msec.2014.05.034 es_ES
dc.description.upvformatpinicio 264 es_ES
dc.description.upvformatpfin 272 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 42 es_ES
dc.identifier.pmid 25063118 es_ES
dc.relation.pasarela S\286286 es_ES
dc.contributor.funder Ministry of Science, Education and Sports, Croacia es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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