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A Temperature-Jump Optical Trap for Single-Molecule Manipulation

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A Temperature-Jump Optical Trap for Single-Molecule Manipulation

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dc.contributor.author de Lorenzo, S. es_ES
dc.contributor.author Ribezzi-Crivellari, M. es_ES
dc.contributor.author Arias-Gonzalez, J. R. es_ES
dc.contributor.author Smith, S.B. es_ES
dc.contributor.author Ritort, F. es_ES
dc.date.accessioned 2020-10-22T03:32:16Z
dc.date.available 2020-10-22T03:32:16Z
dc.date.issued 2015-06-16 es_ES
dc.identifier.issn 0006-3495 es_ES
dc.identifier.uri http://hdl.handle.net/10251/152807
dc.description.abstract [EN] To our knowledge, we have developed a novel temperature-jump optical tweezers setup that changes the temperature locally and rapidly. It uses a heating laser with a wavelength that is highly absorbed by water so it can cover a broad range of temperatures. This instrument can record several force-distance curves for one individual molecule at various temper- atures with good thermal and mechanical stability. Our design has features to reduce convection and baseline shifts, which have troubled previous heating-laser instruments. As proof of accuracy, we used the instrument to carry out DNA unzipping experi- ments in which we derived the average basepair free energy, entropy, and enthalpy of formation of the DNA duplex in a range of temperatures between 5 C and 50 C. We also used the instrument to characterize the temperature-dependent elasticity of single-stranded DNA (ssDNA), where we find a significant condensation plateau at low force and low temperature. Oddly, the persistence length of ssDNA measured at high force seems to increase with temperature, contrary to simple entropic models. es_ES
dc.description.sponsorship The authors thank J. Camunas and S. Frutos for contributing the molecules used in the experiments, and J.M. Huguet for helpful discussion. F.R. is supported by grant Institucio Catalana de Recerca i Estudis Avancats Academia 2013 and J.R. A.-G. by an Explora grant from MINECO (MAT2013-49455-EXP). The research that led to the results presented here was funded by the European Union Seventh Framework Programme (FP7/2007-2013) under grant 308850 INFERNOS and European Research Council grant MagReps (No. 267862). es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Biophysical Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Optical Tweezers es_ES
dc.subject Single Molecule Experiments es_ES
dc.subject Thermodynamics es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title A Temperature-Jump Optical Trap for Single-Molecule Manipulation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.bpj.2015.05.017 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/267862/EU/High-resolution tweezers for DNA replication and sequence identification/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2013-49455-EXP/ES/G-CUADRUPLEX COMO INTERRUPTOR MOLECULAR CONTROLADO POR NANOPARTICULAS Y DEMOSTRADO POR PINZAS OPTICAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/308850/EU/Information, Fluctuations, and Energy Control in Small Systems/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation De Lorenzo, S.; Ribezzi-Crivellari, M.; Arias-Gonzalez, JR.; Smith, S.; Ritort, F. (2015). A Temperature-Jump Optical Trap for Single-Molecule Manipulation. Biophysical Journal. 108(12):2854-2864. https://doi.org/10.1016/j.bpj.2015.05.017 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.bpj.2015.05.017 es_ES
dc.description.upvformatpinicio 2854 es_ES
dc.description.upvformatpfin 2864 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 108 es_ES
dc.description.issue 12 es_ES
dc.identifier.pmid 26083925 es_ES
dc.identifier.pmcid PMC4472040 es_ES
dc.relation.pasarela S\407993 es_ES
dc.contributor.funder Institució Catalana de Recerca i Estudis Avançats es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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