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A DNA-centered explanation of the DNA polymerase translocation mechanism

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A DNA-centered explanation of the DNA polymerase translocation mechanism

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dc.contributor.author Arias-Gonzalez, J. R. es_ES
dc.date.accessioned 2020-10-23T03:31:18Z
dc.date.available 2020-10-23T03:31:18Z
dc.date.issued 2017-08-08 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10251/153037
dc.description.abstract [EN] DNA polymerase couples chemical energy to translocation along a DNA template with a specific directionality while it replicates genetic information. According to single-molecule manipulation experiments, the polymerase-DNA complex can work against loads greater than 50 pN. It is not known, on the one hand, how chemical energy is transduced into mechanical motion, accounting for such large forces on sub-nanometer steps, and, on the other hand, how energy consumption in fidelity maintenance integrates in this non-equilibrium cycle. Here, we propose a translocation mechanism that points to the flexibility of the DNA, including its overstretching transition, as the principal responsible for the DNA polymerase ratcheting motion. By using thermodynamic analyses, we then find that an external load hardly affects the fidelity of the copying process and, consequently, that translocation and fidelity maintenance are loosely coupled processes. The proposed translocation mechanism is compatible with single-molecule experiments, structural data and stereochemical details of the DNA- protein complex that is formed during replication, and may be extended to RNA transcription. es_ES
dc.description.sponsorship The author thanks B. Ibarra and F.J. Cao for fruitful discussion and H.Rodriguez-Rodriguez for critical reading of the manuscript. This work was supported the Spanish Ministry of Economy and Competitiveness (grant number MAT2015-71806-R). es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject DNA es_ES
dc.subject Polymerase es_ES
dc.subject Single molecule es_ES
dc.subject Mechano-chemistry es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title A DNA-centered explanation of the DNA polymerase translocation mechanism es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41598-017-08038-2 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2015-71806-R/ES/INFLUENCIA DEL CALOR EMITIDO POR NANOPARTICULAS MAGNETICAS SOBRE BIOMOLECULAS DETERMINADO MEDIANTE PINZAS OPTICAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.description.bibliographicCitation Arias-Gonzalez, JR. (2017). A DNA-centered explanation of the DNA polymerase translocation mechanism. Scientific Reports. 7:1-8. https://doi.org/10.1038/s41598-017-08038-2 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41598-017-08038-2 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 8 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.identifier.pmid 28790383 es_ES
dc.identifier.pmcid PMC5548866 es_ES
dc.relation.pasarela S\407986 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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