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dc.contributor.author | Jaime-Perez, Noelia | es_ES |
dc.contributor.author | Pineda Chaza, Benito José | es_ES |
dc.contributor.author | García Sogo, Begoña | es_ES |
dc.contributor.author | Atarés Huerta, Alejandro | es_ES |
dc.contributor.author | Athman, Asmini | es_ES |
dc.contributor.author | Byrt, Caitlin S. | es_ES |
dc.contributor.author | Olias, Raquel | es_ES |
dc.contributor.author | Asins, Maria José | es_ES |
dc.contributor.author | Gilliham, Matthew | es_ES |
dc.contributor.author | Moreno Ferrero, Vicente | es_ES |
dc.contributor.author | Belver, Andrés | es_ES |
dc.date.accessioned | 2020-10-28T04:32:29Z | |
dc.date.available | 2020-10-28T04:32:29Z | |
dc.date.issued | 2017-05 | es_ES |
dc.identifier.issn | 0140-7791 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/153356 | |
dc.description.abstract | [EN] Excessive soil salinity diminishes crop yield and quality. In a previous study in tomato, we identified two closely linked genes encoding HKT1-like transporters, HKT1;1 and HKT1;2, as candidate genes for a major quantitative trait locus (kc7.1) related to shoot Na+/K+ homeostasis - a major salt tolerance trait - using two populations of recombinant inbred lines (RILs). Here, we determine the effectiveness of these genes in conferring improved salt tolerance by using two near-isogenic lines (NILs) that were homozygous for either the Solanum lycopersicum allele (NIL17) or for the Solanum cheesmaniae allele (NIL14) at both HKT1 loci; transgenic lines derived from these NILs in which each HKT1;1 and HKT1;2 had been silenced by stable transformation were also used. Silencing of ScHKT1;2 and SlHKT1;2 altered the leaf Na+/K+ ratio and caused hypersensitivity to salinity in plants cultivated under transpiring conditions, whereas silencing SlHKT1;1/ScHKT1;1 had a lesser effect. These results indicate that HKT1;2 has the more significant role in Na+ homeostasis and salinity tolerance in tomato. | es_ES |
dc.description.sponsorship | We thank Dr Espen Granum for critically reading the manuscript, Maria Isabel Gaspar Vidal and Elena Sanchez Romero for technical assistance, the Instrumental Technical Service at EEZ-CSIC for DNA sequencing and ICP-OES mineral analysis and Michael O'Shea for proofreading the text. In addition, we thank Dr Ana P. Ortega who assisted in preliminary experiments. This work was supported by ERDF-cofinanced grants, AGL2010-17090 and AGL2013-41733-R (A.B.), AGL2015-64991-C3-3-R (V.M.) and AGL2014-56675-R (M.J.A.) from the Spanish "Ministerio de Economia, Industria y Competitividad'; CVI-7558, Proyecto de Excelencia, from Junta de Andalucia (A.B); and the Australian Research Council (ARC) for Centre of Excellence (CE14010008) and Future Fellowship (FT130100709) funding (M.G.). N.J-P. was supported by an FPI program BES-2011-046096 and her stay in M.G.'s lab by a short-stay EEBB-I-14-08682, both from the Spanish from "Ministerio de Economia Industria y Competitividad'. The authors have no conflict of interest to declare. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Blackwell Publishing | es_ES |
dc.relation.ispartof | Plant Cell & Environment | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | HKT1,2 | es_ES |
dc.subject | Post-transcriptional gene silencing | es_ES |
dc.subject | Solanum lycopersicum and Solanum cheesmaniae | es_ES |
dc.subject.classification | GENETICA | es_ES |
dc.title | The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1111/pce.12883 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ARC/Future Fellowships/FT130100709/AU/Future Fellowships - Grant ID: FT130100709/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//AGL2010-17090/ES/TRANSPORTE DE NA+ Y K+ A LARGA DISTANCIA EN TOMATE BAJO CONDICIONES SALINAS: ASPECTOS BIOTECNOLOGICOS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2013-41733-R/ES/HOMEOSTASIS DE NA+ Y K+ POR TRANSPORTADORES DE TIPO HKT1, SOS1 Y NHAD Y SU PAPEL EN LA TOLERANCIA DEL TOMATE A LA SALINIDAD/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ARC/Centre of Excellence/CE14010008/AU/Grant ID: CE14010008/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2014-56675-R/ES/APROVECHAMIENTO DE RECURSOS FITOGENETICOS PARA LA DIVERSIFICACION DE PATRONES EFICIENTES EN LA ADQUISICION DE HIERRO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ARC/Future Fellowships/FT130100709/AU/Grant ID: FT130100709/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/Junta de Andalucía//CVI-7558/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BES-2011-046096/ES/BES-2011-046096/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2015-64991-C3-3-R/ES/GENOMICA FUNCIONAL Y MEJORA GENETICA DE TOMATE: IMPORTANCIA AGRONOMICA DEL BALANCE DESARROLLO - ESTRES ABIOTICO/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Jaime-Perez, N.; Pineda Chaza, BJ.; García Sogo, B.; Atarés Huerta, A.; Athman, A.; Byrt, CS.; Olias, R.... (2017). The sodium transporter encoded by the HKT1;2 gene modulates sodium/potassium homeostasis in tomato shoots under salinity. Plant Cell & Environment. 40(5):658-671. https://doi.org/10.1111/pce.12883 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1111/pce.12883 | es_ES |
dc.description.upvformatpinicio | 658 | es_ES |
dc.description.upvformatpfin | 671 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 40 | es_ES |
dc.description.issue | 5 | es_ES |
dc.identifier.pmid | 27987209 | es_ES |
dc.relation.pasarela | S\351705 | es_ES |
dc.contributor.funder | Junta de Andalucía | es_ES |
dc.contributor.funder | Australian Research Council | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |