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dc.contributor.author | Gutiérrez, I. | es_ES |
dc.contributor.author | Garavís, M. | es_ES |
dc.contributor.author | de Lorenzo, S. | es_ES |
dc.contributor.author | Villasante, A. | es_ES |
dc.contributor.author | González, C. | es_ES |
dc.contributor.author | Arias-Gonzalez, J. R. | es_ES |
dc.date.accessioned | 2020-10-31T04:32:29Z | |
dc.date.available | 2020-10-31T04:32:29Z | |
dc.date.issued | 2018-05-17 | es_ES |
dc.identifier.issn | 1948-7185 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/153789 | |
dc.description | "This document is the Accepted Manuscript version of a Published Work that appeared in final form in The Journal of Physical Chemistry Letters, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jpclett.8b00722." | es_ES |
dc.description.abstract | [EN] TERRA is an RNA molecule transcribed from human subtelomeric regions toward chromosome ends potentially involved in regulation of heterochromatin stability, semiconservative replication, and telomerase inhibition, among others. TERRA contains tandem repeats of the sequence GGGUUA, with a strong tendency to fold into a four-stranded arrangement known as a parallel G-quadruplex. Here, we demonstrate by using single-molecule force spectroscopy that this potential is limited by the inherent capacity of RNA to self-associate randomly and further condense into entropically more favorable structures. We stretched RNA constructions with more than four and less than eight hexanucleotide repeats, thus unable to form several G-quadruplexes in tandem, flanked by non-G-rich overhangs of random sequence by optical tweezers on a one by one basis. We found that condensed RNA stochastically blocks G-quadruplex folding pathways with a near 20% probability, a behavior that is not found in DNA analogous molecules. | es_ES |
dc.description.sponsorship | Dedicated to the memory of Alfredo Villasante. The authors thank E. Poyatos-Racionero for auxiliary experiments. I.G. is supported by Fundacion IMDEA Nanociencia and M.G. by a Juan de la Cierva contract (FJCI-2016-28474). This research received grants from the MINECO (MAT2015-71806-R and BFU2017-89707-P). IMDEA Nanociencia acknowledges support from the "Severo Ochoa" Programme for Centres of Excellence in R&D (MINECO, Grant SEV-2016-0686). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation.ispartof | The Journal of Physical Chemistry Letters | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | TERRA | es_ES |
dc.subject | G-quadruplex | es_ES |
dc.subject | RNA | es_ES |
dc.subject | DNA | es_ES |
dc.subject | Optical tweezers | es_ES |
dc.subject | Single-molecule | es_ES |
dc.subject | Folding | es_ES |
dc.subject.classification | FISICA APLICADA | es_ES |
dc.title | Single-Stranded Condensation Stochastically Blocks G-Quadruplex Assembly in Human Telomeric RNA | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1021/acs.jpclett.8b00722 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//MAT2015-71806-R/ES/INFLUENCIA DEL CALOR EMITIDO POR NANOPARTICULAS MAGNETICAS SOBRE BIOMOLECULAS DETERMINADO MEDIANTE PINZAS OPTICAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//SEV-2016-0686/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-89707-P/ES/ESTRUCTURAS CUADRUPLEX COMO DIANA FARMACOLOGICA. NUEVAS OPORTUNIDADES TERAPEUTICAS EN BIOMEDICINA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//FJCI-2016-28474/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada | es_ES |
dc.description.bibliographicCitation | Gutiérrez, I.; Garavís, M.; De Lorenzo, S.; Villasante, A.; González, C.; Arias-Gonzalez, JR. (2018). Single-Stranded Condensation Stochastically Blocks G-Quadruplex Assembly in Human Telomeric RNA. The Journal of Physical Chemistry Letters. 9(10):2498-2503. https://doi.org/10.1021/acs.jpclett.8b00722 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1021/acs.jpclett.8b00722 | es_ES |
dc.description.upvformatpinicio | 2498 | es_ES |
dc.description.upvformatpfin | 2503 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 9 | es_ES |
dc.description.issue | 10 | es_ES |
dc.identifier.pmid | 29688724 | es_ES |
dc.relation.pasarela | S\407983 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |