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Induction of auxin biosynthesis and WOX5 repression mediate changes in root development in Arabidopsis exposed to chitosan

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Induction of auxin biosynthesis and WOX5 repression mediate changes in root development in Arabidopsis exposed to chitosan

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dc.contributor.author Lopez-Moya, Federico es_ES
dc.contributor.author Escudero, Nuria es_ES
dc.contributor.author Zavala-Gonzalez, Ernesto A. es_ES
dc.contributor.author Esteve-Bruna, David es_ES
dc.contributor.author BLAZQUEZ RODRIGUEZ, MIGUEL ANGEL es_ES
dc.contributor.author ALABADÍ DIEGO, DAVID es_ES
dc.contributor.author Lopez-Llorca, Luis V. es_ES
dc.date.accessioned 2020-11-05T04:33:50Z
dc.date.available 2020-11-05T04:33:50Z
dc.date.issued 2017-12-01 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10251/154117
dc.description.abstract [EN] Chitosan is a natural polymer with applications in agriculture, which causes plasma membrane permeabilisation and induction of intracellular reactive oxygen species (ROS) in plants. Chitosan has been mostly applied in the phylloplane to control plant diseases and to enhance plant defences, but has also been considered for controlling root pests. However, the effect of chitosan on roots is virtually unknown. In this work, we show that chitosan interfered with auxin homeostasis in Arabidopsis roots, promoting a 2-3 fold accumulation of indole acetic acid (IAA). We observed chitosan dose-dependent alterations of auxin synthesis, transport and signalling in Arabidopsis roots. As a consequence, high doses of chitosan reduce WOX5 expression in the root apical meristem and arrest root growth. Chitosan also propitiates accumulation of salicylic (SA) and jasmonic (JA) acids in Arabidopsis roots by induction of genes involved in their biosynthesis and signalling. In addition, high-dose chitosan irrigation of tomato and barley plants also arrests root development. Tomato root apices treated with chitosan showed isodiametric cells respect to rectangular cells in the controls. We found that chitosan causes strong alterations in root cell morphology. Our results highlight the importance of considering chitosan dose during agronomical applications to the rhizosphere. es_ES
dc.description.sponsorship This work was supported by AGL 2015 66833-R Grant from the Spanish Ministry of Economy and Competitiveness Grant AGL 2015. We would like to thank Drs Isabel Lopez-Diaz and Esther Carrera for plant hormone quantitation (IBMCP, Valencia, Spain). Part of this work was filed for a patent (P201431399) by L. V. Lopez-Llorca, F. Lopez-Moya and N. Escudero as inventors. We would like to thank Dr Michael Kershaw (University of Exeter) for his English revision and critical comments of the manuscript. We also thank Ms Marta Suarez-Fernandez (University of Alicante) and Mr Alfonso Prieto for their technical support. All the authors reviewed and approved the manuscript. es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.title Induction of auxin biosynthesis and WOX5 repression mediate changes in root development in Arabidopsis exposed to chitosan es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41598-017-16874-5 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2015-66833-R/ES/MODULACION DE INTERACCIONES RIZOSFERICAS PARA PROMOVER CRECIMIENTO, COSECHA Y DEFENSAS FRENTE A PATOGENOS RADICULARES DE CULTIVOS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Lopez-Moya, F.; Escudero, N.; Zavala-Gonzalez, EA.; Esteve-Bruna, D.; Blazquez Rodriguez, MA.; Alabadí Diego, D.; Lopez-Llorca, LV. (2017). Induction of auxin biosynthesis and WOX5 repression mediate changes in root development in Arabidopsis exposed to chitosan. Scientific Reports. 7:1-14. https://doi.org/10.1038/s41598-017-16874-5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41598-017-16874-5 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 14 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.identifier.pmid 29196703 es_ES
dc.identifier.pmcid PMC5711845 es_ES
dc.relation.pasarela S\356352 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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