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Cold seawater induces early maturational stages in the BPG axis of European eel males

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Cold seawater induces early maturational stages in the BPG axis of European eel males

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dc.contributor.author Rozenfeld, Christoffer es_ES
dc.contributor.author García-Carpintero-Burgos, Víctor es_ES
dc.contributor.author Pérez Igualada, Luz María es_ES
dc.contributor.author Gallego Albiach, Victor es_ES
dc.contributor.author Herranz-Jusdado, Juan Germán es_ES
dc.contributor.author Tveiten, Helge es_ES
dc.contributor.author Johnsen, Helge K. es_ES
dc.contributor.author Fontaine, Romain es_ES
dc.contributor.author Weltzien, F.A. es_ES
dc.contributor.author Cañizares Sales, Joaquín es_ES
dc.contributor.author Asturiano, Juan F. es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.date.accessioned 2020-11-07T04:32:23Z
dc.date.available 2020-11-07T04:32:23Z
dc.date.issued 2019-07-22 es_ES
dc.identifier.issn 1471-2164 es_ES
dc.identifier.uri http://hdl.handle.net/10251/154383
dc.description.abstract [EN] BackgroundThe impossibility of closing the life cycle of the European eel (Anguilla anguilla) in captivity troubles the future of this critically endangered species. In addition, the European eel is a highly valued and demanded resource, thus the successful closing of its life cycle would have a substantial economic and ecological impact. With the aim of obtaining the highest gamete quality, the study of the effects of environmental factors, such as temperature, on reproductive performance may prove valuable. This is especially true for the exposure to cold water, which has been reported to improve sexual development in multiple other Actinopterygii species.ResultsEuropean eel males treated with cold seawater (10 degrees C, T10) for 2weeks showed an increase in the proliferation and differentiation of spermatogonial cells until the differentiated spermatogonial type A cell stage, and elevated testosterone and 11-ketotestosterone plasma levels. Transcriptomes from the tissues of the brain-pituitary-gonad (BPG) axis of T10 samples revealed a differential gene expression profile compared to the other experimental groups, with clustering in a principal component analysis and in heat maps of all differentially expressed genes. Furthermore, a functional analysis of differentially expressed genes revealed enriched gene ontology terms involved in the regulation of circadian rhythm, histone modification, meiotic nuclear division, and others.ConclusionsCold seawater treatment had a clear effect on the activity of the BPG-axis of European eel males. In particular, our cold seawater treatment induces the synchronization and increased proliferation and differentiation of specific spermatogonial cells. In the transcriptomic results, genes related to thermoception were observed. This thermoception may have caused the observed effects through epigenetic mechanisms, since all analysed tissues further revealed differentially expressed genes involved in histone modification. The presented results support our hypothesis that a low temperature seawater treatment induces an early sexual developmental stage in European eels. This hypothesis is logical given that the average temperature experienced by eels in the early stages of their oceanic reproductive migration is highly similar to that of this cold seawater treatment. Further studies are needed to test whether a cold seawater treatment can improve the response of European eels to artificial hormonal treatment, as the results suggest. es_ES
dc.description.sponsorship This work was subsidized by the Spanish Ministry of Science and Innovation (REPRO-TEMP project, AGL2013-41646-R), the Spanish Ministry of Innovation, Science and Universities (EELGONIA project, RTI2018-096413-B-I00), and by the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 642893 (IMPRESS), including the CR and JGHJ predoctoral contracts. JGHJ was granted a Short Term Scientific Mission by the COST Office (COST Action AQUAGAMETE: Assessing and improving the quality of aquatic animal gametes to enhance aquatic resources. The need to harmonize and standardize evolving methodologies, and improve transfer from academia to industry). The funding bodies had no role in the study, analysis, interpretation of data, or in writing the manuscript. es_ES
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation info:eu-repo/grantAgreement/MINECO//AGL2013-41646-R/ES/LA ANGUILA EUROPEA COMO MODELO PARA ESTUDIAR LA TEMPERATURA COMO MODULADOR DE LA MADURACION SEXUAL EN TELEOSTEOS. POTENCIAL APLICACION EN ACUICULTURA./ es_ES
dc.relation CEE/CGA-FA1205-2 es_ES
dc.relation info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096413-B-I00/ES/DESARROLLO Y APLICACION DE LAS TECNICAS DE PRODUCCION SUBROGADA DE GAMETOS DE ANGUILA EUROPEA MEDIANTE XENOTRASPLANTE DE ESPERMATOGONIAS EN OTRAS ESPECIES DE PECES/ es_ES
dc.relation.ispartof BMC Genomics es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Anguilla anguilla es_ES
dc.subject RNA-sequencing es_ES
dc.subject Epigenetics es_ES
dc.subject Temperature es_ES
dc.subject Spermatogonial proliferation es_ES
dc.subject Migration es_ES
dc.subject Immunofluorescence es_ES
dc.subject Radioimmunoassay es_ES
dc.subject Histology es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.subject.classification GENETICA es_ES
dc.title Cold seawater induces early maturational stages in the BPG axis of European eel males es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12864-019-5969-6 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/642893/EU/Improved production strategies for endangered freshwater species./ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Ciencia y Tecnología Animal - Institut de Ciència i Tecnologia Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Rozenfeld, C.; García-Carpintero-Burgos, V.; Pérez Igualada, LM.; Gallego Albiach, V.; Herranz-Jusdado, JG.; Tveiten, H.; Johnsen, HK.... (2019). Cold seawater induces early maturational stages in the BPG axis of European eel males. BMC Genomics. 20:1-20. https://doi.org/10.1186/s12864-019-5969-6 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1186/s12864-019-5969-6 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 20 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 20 es_ES
dc.identifier.pmid 31331264 es_ES
dc.identifier.pmcid PMC6647157 es_ES
dc.relation.pasarela S\392202 es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Ministerio de Economía y Empresa es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder European Cooperation in Science and Technology es_ES
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