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Tomato glycosyltransferase Twi1 plays a role in flavonoid glycosylation and defence against virus

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Tomato glycosyltransferase Twi1 plays a role in flavonoid glycosylation and defence against virus

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dc.contributor.author Campos Beneyto, Laura es_ES
dc.contributor.author López-Gresa, María Pilar es_ES
dc.contributor.author Fuertes, Diana es_ES
dc.contributor.author Belles Albert, José Mª es_ES
dc.contributor.author Rodrigo Bravo, Ismael es_ES
dc.contributor.author Lisón, Purificación es_ES
dc.date.accessioned 2020-11-07T04:32:37Z
dc.date.available 2020-11-07T04:32:37Z
dc.date.issued 2019-10-26 es_ES
dc.identifier.issn 1471-2229 es_ES
dc.identifier.uri http://hdl.handle.net/10251/154388
dc.description.abstract [EN] Background: Secondary metabolites play an important role in the plant defensive response. They are produced as a defence mechanism against biotic stress by providing plants with antimicrobial and antioxidant weapons. In higher plants, the majority of secondary metabolites accumulate as glycoconjugates. Glycosylation is one of the commonest modifications of secondary metabolites, and is carried out by enzymes called glycosyltransferases. Results: Here we provide evidence that the previously described tomato wound and pathogen-induced glycosyltransferase Twi1 displays in vitro activity toward the coumarins scopoletin, umbelliferone and esculetin, and the flavonoids quercetin and kaempferol, by uncovering a new role of this gene in plant glycosylation. To test its activity in vivo, Twi1-silenced transgenic tomato plants were generated and infected with Tomato spotted wilt virus. The Twi1- silenced plants showed a differential accumulation of Twi1 substrates and enhanced susceptibility to the virus. Conclusions: Biochemical in vitro assays and transgenic plants generation proved to be useful strategies to assign a role of tomato Twi1 in the plant defence response. Twi1 glycosyltransferase showed to regulate quercetin and kaempferol levels in tomato plants, affecting plant resistance to viral infection. es_ES
dc.description.sponsorship This work was supported by grant BIO2012-33419 from the Direccion General de Programas y Transferencia de Conocimiento, Spanish Ministry of Science and Innovation, and grant AICO/2017/048 from the Valencian Local Government (Generalitat Valenciana, Spain). LC was supported by a predoctoral fellowship (ACIF/2010/231) from the Valencian Local Government (Generalitat Valenciana, Spain). None of the funding bodies was involved in the design of the study, the collection, analysis, and interpretation of the data, nor in the writing of the manuscript, which was made entirely by the authors. es_ES
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Glycosyltransferase es_ES
dc.subject Tomato wound-induced gene es_ES
dc.subject Coumarins es_ES
dc.subject Flavonoids es_ES
dc.subject Pathogen es_ES
dc.subject Transgenic plants es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Tomato glycosyltransferase Twi1 plays a role in flavonoid glycosylation and defence against virus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12870-019-2063-9 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2012-33419/ES/CARACTERIZACION DE GENES Y METABOLITOS IMPLICADOS EN LA RESPUESTA DEFENSIVA DE LAS PLANTAS FRENTE A PATOGENOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//ACIF%2F2010%2F231/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//AICO%2F2017%2F048/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Campos Beneyto, L.; López-Gresa, MP.; Fuertes, D.; Belles Albert, JM.; Rodrigo Bravo, I.; Lisón, P. (2019). Tomato glycosyltransferase Twi1 plays a role in flavonoid glycosylation and defence against virus. BMC Plant Biology. 19:1-17. https://doi.org/10.1186/s12870-019-2063-9 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1186/s12870-019-2063-9 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 19 es_ES
dc.identifier.pmid 31655554 es_ES
dc.identifier.pmcid PMC6815406 es_ES
dc.relation.pasarela S\394949 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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