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dc.contributor.author | Leastro, Mikhail O. | es_ES |
dc.contributor.author | De Oliveira, Athos Silva | es_ES |
dc.contributor.author | Pallás Benet, Vicente | es_ES |
dc.contributor.author | SANCHEZ NAVARRO, JESUS ANGEL | es_ES |
dc.contributor.author | Kormelink, Richard | es_ES |
dc.contributor.author | Resende, Renato Oliveira | es_ES |
dc.date.accessioned | 2020-11-17T04:32:39Z | |
dc.date.available | 2020-11-17T04:32:39Z | |
dc.date.issued | 2017-08-15 | es_ES |
dc.identifier.issn | 0168-1702 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/155127 | |
dc.description.abstract | [EN] The cell-to-cell movement protein (NSM) in members of the tospovirus species Tomato spotted wilt virus (TSWV) has been recently identified as the effector of the single dominant Sw-5b resistance gene from tomato (Solanum lycopersicum L.). Although most TSWV isolates shows a resistance-inducing (RI) phenotype, regular reports have appeared on the emergence of resistance-breaking (RB) isolates in tomato fields, and suggested a strong association with two point mutations (C118Y and T120N) in the NSM protein. In this study the Sw-5b gene has been demonstrated to confer not only resistance against TSWV but to members of five additional, phylogeneticallyrelated tospovirus species classified within the so-called ¿American¿ evolutionary clade, i.e. Alstroemeria necrotic streak virus (ANSV), Chrysanthemum stem necrosis virus (CSNV), Groundnut ringspot virus (GRSV), Impatiens necrotic spot virus (INSV) and Tomato chlorotic spot virus (TCSV). Remarkably, a member of the species Bean necrotic mosaic virus (BeNMV), a recently discovered tospovirus classified in a distinct American subclade and circulating on the American continent, did not trigger a Sw-5b-mediated hypersensitive (HR) response. Introduction of point mutations C118Y and T120N into the NSM protein of TSWV, TCSV and CSNV abrogated the ability to trigger Sw-5b-mediated HR in both transgenic-N. benthamiana and tomato isolines harboring the Sw-5b gene whereas it had no effect on BeNMV NSM. Truncated versions of TSWV NSM lacking motifs associated with tubule formation, cell-to-cell or systemic viral movement were made and tested for triggering of resistance. HR was still observed with truncated NSM proteins lacking 50 amino acids (out of 301) from either the amino- or carboxy-terminal end. These data altogether indicate the importance of amino acid residues C118 and T120 in Sw-5b-mediated HR only for the NSM proteins from one cluster of tospoviruses within the American clade, and that the ability to support viral cell-to-cell movement is not required for effector functionality | es_ES |
dc.description.sponsorship | We thank Leonardo Boiteux (Embrapa Vegetable Crops - CNPH, Brasilia, DF, Brazil) for providing the isogenic line "CNPH LAM 147" essential to elaboration of this work. This research was financially supported by CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), CAPES (Conselho de Aperfeicoamento de Pessoal de Nivel Superior), BIO2014-54862-R from the Spanish Direccion General de Investigacion Cientifica y Tecnica (DGICYT), the Prometeo Program GV2014/010 from the Generalitat Valenciana and Wageningen University | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Virus Research | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Resistance | es_ES |
dc.subject | Sw-5b gene | es_ES |
dc.subject | NSM protein | es_ES |
dc.subject | Tospovirus | es_ES |
dc.subject | Avr | es_ES |
dc.subject | Hypersensitive response | es_ES |
dc.title | The NSm proteins of phylogenetically related tospoviruses trigger Sw-5b-mediated resistance dissociated of their cell-to-cell movement function | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.virusres.2017.07.019 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BIO2014-54862-R/ES/INTERACCIONES ENTRE FACTORES VIRALES Y DEL HUESPED IMPLICADOS EN LOS PROCESOS DE MOVIMIENTO Y PATOGENESIS EN CULTIVOS DE INTERES AGRONOMICO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//GV%2F2014%2F010/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Leastro, MO.; De Oliveira, AS.; Pallás Benet, V.; Sanchez Navarro, JA.; Kormelink, R.; Resende, RO. (2017). The NSm proteins of phylogenetically related tospoviruses trigger Sw-5b-mediated resistance dissociated of their cell-to-cell movement function. Virus Research. 240:25-34. https://doi.org/10.1016/j.virusres.2017.07.019 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.virusres.2017.07.019 | es_ES |
dc.description.upvformatpinicio | 25 | es_ES |
dc.description.upvformatpfin | 34 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 240 | es_ES |
dc.identifier.pmid | 28754561 | es_ES |
dc.relation.pasarela | S\356543 | es_ES |
dc.contributor.funder | Ministerio de Economía, Industria y Competitividad | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior, Brasil | es_ES |
dc.contributor.funder | Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil | es_ES |