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Production of transgenic apricot plants from hypocotyl segments of mature seeds

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Production of transgenic apricot plants from hypocotyl segments of mature seeds

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dc.contributor.author Petri, C. es_ES
dc.contributor.author WANG, HONG es_ES
dc.contributor.author Burgos, L. es_ES
dc.contributor.author SANCHEZ NAVARRO, JESUS ANGEL es_ES
dc.contributor.author Alburquerque, N. es_ES
dc.date.accessioned 2020-11-18T04:31:06Z
dc.date.available 2020-11-18T04:31:06Z
dc.date.issued 2015-12-14 es_ES
dc.identifier.issn 0304-4238 es_ES
dc.identifier.uri http://hdl.handle.net/10251/155234
dc.description.abstract [EN] Apricot (Prunus armeniaca L.) is one of the most recalcitrant species for genetic transformation. In this work; we report the regeneration of transgenic apricot plants from mature seed hypocotyl segments (cv. 'Canino' and 'Moniqui') for the first time. Moreover, this is the first report of the regeneration of apricot transgenic plants by means of the selective bar gene. Agrobacterium tumefaciens strain LB4404 harbouring a binary plasmid with eyfp and nptII genes (pMOG-eyfp-IV2) or a plasmid with gus and bar genes (pFGC5941-gus), was used in this study. A gradual selection strategy with aminoglycoside antibiotics made it possible to produce transgenic plants with 1.5 +/- 0.5% efficiency using 10 mu M paromomycin (PAR). When buds were isolated and placed in a meristem development medium, PAR was changed to 20 mu M kanamycin (KAN). As the buds developed and elongated, the concentration was increased up to 140 mu M KAN based on a 20 mu M increase every 4 weeks. When bar was the marker gene, the regeneration-inhibitory PPT concentration (75.7 mu M) was applied immediately after the co-culture. In this case, the PPT concentration was reduced afterwards to 10.1 mu M in the micropropagation medium. This strategy allowed for the regeneration of transgenic shoots with 3.8 +/- 1.4% efficiency. Transformation events were monitored by detection of the reporter gene (eyfp or gus) expression. PCR and Southern blot analysis confirmed the stable integration of the transgenes into the apricot genome. Transformed shoots were rooted on a selective medium, acclimatised, cultured and raised under greenhouse conditions. Our results confirm that the process used in this study is an efficient transformation system for the apricot species which could be used for functional genomics studies and the development of transgenic rootstocks. (C) 2015 Elsevier B.V. All rights reserved. es_ES
dc.description.sponsorship The authors would like to thank Dr. Ralph Scorza for kindly providing pFGC5941-gus plasmid, and Dr. Jose Egea for providing 'Moniqui' and 'Canino' fruits. This research was supported by the grants AGL2010-20270 and RTA2013-00026-C03-02, cofinanced by FEDER funds and by the grant BIO2011-25018 from the Spanish granting agency DGICYT. C.P. thanks the UPCT and the Spanish Ministry of Economy and Competitiveness for his Ramon & Cajal contract. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Scientia Horticulturae es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Prunus es_ES
dc.subject Agrobacterium es_ES
dc.subject Antibiotic selection es_ES
dc.subject Phosphinothricin es_ES
dc.subject Genetic transformation es_ES
dc.title Production of transgenic apricot plants from hypocotyl segments of mature seeds es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.scienta.2015.09.023 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//BIO2011-25018/ES/TRAFICO INTRACELULAR, INTERCELULAR Y VASCULAR DE RNAS Y PROTEINAS VIRALES Y SUBVIRALES EN PLANTAS¿/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2010-20270/ES/SILENCIAMIENTO DE ONCOGENES DE AGROBACTERIUM TUMEFACIENS PARA INDUCIR RESISTENCIA A LA ENFERMEDAD DE LA AGALLA DE CORONA EN PRUNUS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTA2013-00026-C03-02/ES/Estudio de nuevos mecanismos y estrategias para introducción de resistencia a la sharka./ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Petri, C.; Wang, H.; Burgos, L.; Sanchez Navarro, JA.; Alburquerque, N. (2015). Production of transgenic apricot plants from hypocotyl segments of mature seeds. Scientia Horticulturae. 197:144-149. https://doi.org/10.1016/j.scienta.2015.09.023 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.scienta.2015.09.023 es_ES
dc.description.upvformatpinicio 144 es_ES
dc.description.upvformatpfin 149 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 197 es_ES
dc.relation.pasarela S\323850 es_ES
dc.contributor.funder Universidad Politécnica de Cartagena es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES


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