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Parthenogenic blastocysts cultured under in vivo conditions exhibited proliferation and differentiation expression genes similar to those normal embryos

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Parthenogenic blastocysts cultured under in vivo conditions exhibited proliferation and differentiation expression genes similar to those normal embryos

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dc.contributor.advisor Marco Jiménez, Francisco es_ES
dc.contributor.author Naturil Alfonso, Carmen es_ES
dc.date.accessioned 2012-05-10T06:32:57Z
dc.date.available 2012-05-10T06:32:57Z
dc.date.created 2011-12
dc.date.issued 2012-05-10
dc.identifier.uri http://hdl.handle.net/10251/15572
dc.description.abstract Parthenotes embryos offer multiple possibilities in biotechnological investigations, such as stem cell research. However, there is still a dearth of knowledge of this kind of embryos. In this study, we analyzed development and ploidy in parthenotes under in vitro and in vivo culture conditions. Subsequently, using real-time PCR, we analyzed the expression of factor OCT-4, Vascular Endothelial Growth Factor, Epidermal Growth Factor Receptor 3 and Transforming Growth Factor )2 genes to compare the kind of embryo at the blastocyst stage. Development and implantation of parthenotes embryos were describe after transfer at day 10 of pregnancy. Parthenotes showed similar blastocyst development for both culture conditions and that most of the parthenotes produced were diploid. However, parthenotes developed under in vivo conditions showed similar mRNA expression of OCT-4, VEGF and TGF- 2 to 5 and 6 day old blastocysts. While, parthenotes developed under in vitro conditions had altered the expression pattern of these genes, except for erbB3 mRNA. Finally, transferred parthenotes had the ability to implant but showed severe growth retardation and lesser size. This is the first demonstration of the influence of culture conditions on parthenotes mRNA expression. Our study highlights the importance of culture conditions in the subsquent uses of parthenotes such as the production of stem cell lines. es_ES
dc.format.extent 49 es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Mrna expression es_ES
dc.subject Culture conditions es_ES
dc.subject In vivo es_ES
dc.subject In vitro es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.subject.other Máster Universitario en Biotecnología Biomédica-Màster Universitari en Biotecnologia Biomèdica es_ES
dc.title Parthenogenic blastocysts cultured under in vivo conditions exhibited proliferation and differentiation expression genes similar to those normal embryos es_ES
dc.type Tesis de máster es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Servicio de Alumnado - Servei d'Alumnat es_ES
dc.description.bibliographicCitation Naturil Alfonso, C. (2011). Parthenogenic blastocysts cultured under in vivo conditions exhibited proliferation and differentiation expression genes similar to those normal embryos. http://hdl.handle.net/10251/15572 es_ES
dc.description.accrualMethod Archivo delegado es_ES


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