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BCL2-ASSOCIATED ATHANOGENE4 Regulates the KAT1 Potassium Channel and Controls Stomatal Movement

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BCL2-ASSOCIATED ATHANOGENE4 Regulates the KAT1 Potassium Channel and Controls Stomatal Movement

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dc.contributor.author Locascio, Antonella Anna Maria es_ES
dc.contributor.author Marques Romero, Mª Carmen es_ES
dc.contributor.author García-Martínez, Guillermo es_ES
dc.contributor.author Corratgé-Faillie, Claire es_ES
dc.contributor.author Andrés-Colás, Nuria es_ES
dc.contributor.author Rubio, Lourdes es_ES
dc.contributor.author Fernández, José Antonio es_ES
dc.contributor.author Véry, Anne-Aliénor es_ES
dc.contributor.author Mulet, José Miguel es_ES
dc.contributor.author Yenush, Lynne es_ES
dc.date.accessioned 2020-12-04T04:32:26Z
dc.date.available 2020-12-04T04:32:26Z
dc.date.issued 2019-11 es_ES
dc.identifier.issn 0032-0889 es_ES
dc.identifier.uri http://hdl.handle.net/10251/156426
dc.description.abstract [EN] Potassium (K+) is a key monovalent cation necessary for multiple aspects of cell growth and survival. In plants, this cation also plays a key role in the control of stomatal movement. KAT1 and its homolog KAT2 are the main inward rectifying channels present in guard cells, mediating K+ influx into these cells, resulting in stomatal opening. To gain further insight into the regulation of these channels, we performed a split-ubiquitin protein-protein interaction screen searching for KAT1 interactors in Arabidopsis (Arabidopsis thaliana). We characterized one of these candidates, BCL2-ASSOCIATED ATHANOGENE4 (BAG4), in detail using biochemical and genetic approaches to confirm this interaction and its effect on KAT1 activity. We show that BAG4 improves KAT1-mediated K+ transport in two heterologous systems and provide evidence that in plants, BAG4 interacts with KAT1 and favors the arrival of KAT1 at the plasma membrane. Importantly, lines lacking or overexpressing the BAG4 gene show altered KAT1 plasma membrane accumulation and alterations in stomatal movement. Our data allowed us to identify a KAT1 regulator and define a potential target for the plant BAG family. The identification of physiologically relevant regulators of K+ channels will aid in the design of approaches that may impact drought tolerance and pathogen susceptibility. es_ES
dc.description.sponsorship The authors would like to thank Elena Moreno, María José Falaguer, Alejandro Mossi, Sara Aljama, José Antonio Navarro, Vicente Pallás, Daniel Franco-Aragón, Jorge Lozano, and Stan Gelvin for assistance in the completion of this work and for providing reagents. This work was supported by the Spanish Ministry of Economy and Competitiveness (BIO201677776-P and BIO2016-81957-REDT) and the Valencian Government (AICO/2018/300). es_ES
dc.language Inglés es_ES
dc.publisher American Society of Plant Biologists es_ES
dc.relation MINECO/BIO2016-77776-P es_ES
dc.relation MINECO/BIO2016-81957-REDT es_ES
dc.relation GENERALITAT VALENCIANA/AICO/2018/300 es_ES
dc.relation.ispartof PLANT PHYSIOLOGY es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Plant K+ channel es_ES
dc.subject Endoplasmic-Reticulum es_ES
dc.subject BCL-2-Associated athanogene es_ES
dc.subject Saccharomyces-Cerevisiae es_ES
dc.subject Plasma-Membrane es_ES
dc.subject Abscisic-Acid es_ES
dc.subject Arabidopsis es_ES
dc.subject Expression es_ES
dc.subject Family es_ES
dc.subject Trafficking es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title BCL2-ASSOCIATED ATHANOGENE4 Regulates the KAT1 Potassium Channel and Controls Stomatal Movement es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1104/pp.19.00224 es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Locascio, AAM.; Marques Romero, MC.; García-Martínez, G.; Corratgé-Faillie, C.; Andrés-Colás, N.; Rubio, L.; Fernández, JA.... (2019). BCL2-ASSOCIATED ATHANOGENE4 Regulates the KAT1 Potassium Channel and Controls Stomatal Movement. PLANT PHYSIOLOGY. 181(3):1277-1294. https://doi.org/10.1104/pp.19.00224 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1104/pp.19.00224 es_ES
dc.description.upvformatpinicio 1277 es_ES
dc.description.upvformatpfin 1294 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 181 es_ES
dc.description.issue 3 es_ES
dc.identifier.pmid 31451552 es_ES
dc.identifier.pmcid PMC6836829 es_ES
dc.relation.pasarela S\401414 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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