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Identification of inoculum sources of Fusicladium eriobotryae in loquat orchards in Spain

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Identification of inoculum sources of Fusicladium eriobotryae in loquat orchards in Spain

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dc.contributor.author Elena-Jiménez, Georgina es_ES
dc.contributor.author Berbegal Martinez, Monica es_ES
dc.contributor.author González Domínguez, E. es_ES
dc.contributor.author Armengol Fortí, Josep es_ES
dc.date.accessioned 2020-12-05T04:32:17Z
dc.date.available 2020-12-05T04:32:17Z
dc.date.issued 2020-02 es_ES
dc.identifier.issn 0929-1873 es_ES
dc.identifier.uri http://hdl.handle.net/10251/156501
dc.description.abstract [EN] Fusicladium eriobotryae is the causal agent of loquat scab, the main disease damaging fruit, leaves and young twigs of this crop. A two-growing season study (2015¿2016 and 2016¿2017) was carried out in two loquat orchards (cv ¿Algerie¿) to determine the inoculum sources of F. eriobotryae by direct observation of conidia, pathogen isolation on culture media and detection using a new real time PCR protocol developed in this study. One-year-old twigs, fruit peduncles and fruit mummies were randomly sampled three times per growing season on each orchard, and inflorescences only at flowering. Conidia of F. eriobotryae were not found and the isolation of the pathogen was neither possible from any sample in both seasons. Specific primers FUG2F and FUG2R, were designed to detect and quantify DNA of F. eriobotryae on plant material, with a limit of detection (LOD) established at 48.6 fg/¿l. The DNA of the pathogen was not detected by real time PCR in fruit mummies nor inflorescences. It was detected in fruit peduncles and twigs in the season 2016¿2017 with concentrations ranging from 50 to 2742 fg/¿l, confirming that this two loquat organs might act as potential inoculum sources for F. eriobotryae. The detection of F. eriobotryae only in this season agrees with the predictions of an epidemiological model for this pathogen. Our results indicate that in years with a high disease pressure, fruit twigs and peduncles might act as a source of inoculum of new infections the following year. es_ES
dc.description.sponsorship This study was funded by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) grant number RTA2013-00004-C03-03, and FEDER Funds. G. Elena was supported by the Spanish post-doctoral grant Juan de la Cierva-Formación. We thank the E. Soler from the Cooperativa Agrícola de Callosa d En Sarrià (Alicante, Spain) for his collaboration during orchard sampling, and A. Ramón-Albalat and V. Serra for their technical assistance. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof European Journal of Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Loquat scab es_ES
dc.subject Epidemiology es_ES
dc.subject Pathogen detection es_ES
dc.subject QPCR es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.subject.classification BOTANICA es_ES
dc.title Identification of inoculum sources of Fusicladium eriobotryae in loquat orchards in Spain es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10658-019-01892-y es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTA2013-00004-C03-03/ES/Nuevas herramientas epidemiológicas y moleculares para el manejo de enfermedades fúngicas de la parte aérea en frutales mediterráneos/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//FJCI-2015-24787/ES/FJCI-2015-24787/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.description.bibliographicCitation Elena-Jiménez, G.; Berbegal Martinez, M.; González Domínguez, E.; Armengol Fortí, J. (2020). Identification of inoculum sources of Fusicladium eriobotryae in loquat orchards in Spain. European Journal of Plant Pathology. 156:425-436. https://doi.org/10.1007/s10658-019-01892-y es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s10658-019-01892-y es_ES
dc.description.upvformatpinicio 425 es_ES
dc.description.upvformatpfin 436 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 156 es_ES
dc.relation.pasarela S\402827 es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.description.references Acuña, R. P. (2010). Compendio de bacterias y hongos de frutales y vides en Chile. Santiago de Chile: Servicio Agrícola y Ganadero. es_ES
dc.description.references Bilodeau, G. J., Koike, S. T., Uribe, P., & Martin, F. N. (2012). Development of an assay for rapid detection and quantification of Verticillium dahliae in soil. Phytopathology, 102, 331–343. es_ES
dc.description.references Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., Hipley, G. L., Vandesompele, J., & Wittwer, C. T. (2009). The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry, 55, 611–622. es_ES
dc.description.references Caballero, P., & Fernández, M. A. (2002). Loquat, production and market. Options Méditerranéennes Serie A, 58, 11–20. es_ES
dc.description.references Ciliberti, N., Fermaud, M., Languasco, L., & Rossi, V. (2015). Influence of fungal strain, temperature, and wetness duration of infection of grapevine inflorescences and young berry clusteres by Botrytis cinerea. Phytopathology, 105, 325–333. es_ES
dc.description.references Cullen, D. W., Lees, A. K., Toth, I. K., & Duncan, J. M. (2001). Conventional PCR and real-time quantitative PCR detection of Helminthosporium solani in soil and on potato tubers. European Journal of Plant Pathology, 107, 387–398. es_ES
dc.description.references Daniëls, B., De Landtsheer, A., Dreesen, R., Davey, M. W., & Keulemans, J. (2012). Real-time PCR as a promising tool to monitor growth of Venturia spp. in scab-susceptible and -resistant apple leaves. European Journal of Plant Pathology, 134, 821–833. es_ES
dc.description.references Demaree, J. (1924). Pecan scab with special reference to sources of the early spring infections. Journal of Agriculture Research, 28, 321–330. es_ES
dc.description.references Ghasemkhani, M., Holefors, A., Marttila, S., Dalman, K., Zborowska, A., Rur, M., Rees-George, J., Nybom, H., Everett, K. R., Scheper, R. W. A., & Garkava-Gustavsson, L. (2016). Real-time PCR for detection and quantification, and histological characterization of Neonectria ditissima in apple trees. Trees, 30, 1111–1125. es_ES
dc.description.references Gisbert, A. D., Besoain, X., Llácer, G., & Badenes, M. L. (2006). Protección de cultivo II, Enfermedades. In M. Agustí, C. Reig, & P. Undurraga (Eds.), El Cultivo del Níspero Japonés (pp. 227–246). Valencia: Gráficas Alcoy. es_ES
dc.description.references Gladieux, P., Caffier, V., Devaux, M., & Le Cam, B. (2010). Host specific differentiation among populations of Venturia inaequalis causing scab on apple, pyracantha and loquat. Fungal Genetics and Biology, 47, 511–521. es_ES
dc.description.references González-Domínguez, E., Rossi, V., Armengol, J., & García-Jiménez, J. (2013). Effect of environmental factors on mycelial growth and conidial germination of Fusicladium eriobotryae, and the infection of loquat leaves. Plant Disease, 97, 1331–1338. es_ES
dc.description.references González-Domínguez, E., Armengol, J., & Rossi, V. (2014a). Development and validation of a weather-based model for predicting infection of loquat fruit by Fusicladium eriobotryae. PLoS One, 9, e107547. es_ES
dc.description.references González-Domínguez, E., Rossi, V., Michereff, S. J., García-Jiménez, J., & Armengol, J. (2014b). Dispersal of conidia of Fusicladium eriobotryae and spatial patterns of scab in loquat orchards in Spain. European Journal of Plant Pathology, 139, 849–861. es_ES
dc.description.references González-Domínguez, E., León, M., Armengol, J., & Berbegal, M. (2015). A nested polymerase chain reaction protocol for in planta detection of Fusicladium eriobotryae, causal agent of loquat scab. Journal of Phytopathology, 163, 415–418. es_ES
dc.description.references González-Domínguez, E., Armengol, J., & Rossi, V. (2017). Biology and epidemiology of Venturia species affecting fruit crops: A review. Frontiers in Plant Science, 8, 1496. es_ES
dc.description.references Graniti, A. (1993). Olive scab: A review. EPPO Bulletin, 23, 377–384. es_ES
dc.description.references Gusberti, M., Patocchi, A., Gessler, C., & Broggini, G. A. L. (2012). Quantification of Venturia inaequalis growth in Malus × domestica with quantitative real-time polymerase chain reaction. Plant Disease, 96, 1791–1797. es_ES
dc.description.references Janick, J. (2011). Predictions for loquat improvement in the next decade. Acta Horticulturae, 887, 25–30. es_ES
dc.description.references Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874. es_ES
dc.description.references Lalancette, N., McFarland, K., & Burnett, L. (2012). Modelling sporulation of Fusicladium carpophilum on nectarine twig lesions: Relative humidity and temperature effects. Phytopathology, 102, 421–428. es_ES
dc.description.references Lin, S. Q. (2007). World loquat production and research with special reference to China. Acta Horticulturae, 750, 37–44. es_ES
dc.description.references Martínez-Calvo, B. J., Badenes, M. L., Llacer, G., Bleiholder, H., Hack, H., & Meier, U. (1999). Phenological growth stages of loquat tree (Eriobotrya japonica (Thunb) Lindl.). Annals of Applied Biology, 134, 353–357. es_ES
dc.description.references Pilotti, M., Lumia, V., Di Lernia, G., & Brunetti, A. (2012). Development of real-time PCR for in wood-detection of Ceratocystis platani, the agent of canker stain of Platanus spp. European Journal of Plant Pathology, 134, 61–79. es_ES
dc.description.references Prota, U. (1960). Ricerche sulla «ticchiolatura del Nespolo del Giappone e sul suo agente (Fusicladium eriobotryae Cav.). I. Observazioni sull’epidemiologia della malattia e sui caratteri morfo-biologici del parassita in Sardegna. Studi di Sassari, 8, 175–196. es_ES
dc.description.references Ptskialadze, L. (1968). The causal agent of loquat scab and its biological characteristics. Review of Applied Mycology, 47, 268. es_ES
dc.description.references Raabe, R., & Gardner, M. W. (1972). Scab of pyracantha, loquat, Toyon and Kageneckia. Phytopathology, 62, 914–916. es_ES
dc.description.references Rodríguez, A. (1983). El cultivo del níspero en el valle del Algar-Guadalest. Sociedad Cooperativa de Crédito de. Alicante: Callosa d’En Sarrià. es_ES
dc.description.references Salerno, M., Somma, V., & Rosciglione, B. (1971). Ricerche sull’epidemiologia della ticchiolatura del nespolo del giappone. Technology Agriculture, 23, 947–956. es_ES
dc.description.references Sánchez-Torres, P., Hinarejos, R., & Tuset, J. J. (2007a). Fusicladium eriobotryae: hongo causante del moteado del níspero en el Mediterráneo español. Boletín de Sanidad Vegetal. Plagas, 33, 89–98. es_ES
dc.description.references Sánchez-Torres, P., Hinarejos, R., & Tuset, J. J. (2007b). Identification and characterization of Fusicladium eriobotryae: Fungal pathogen causing mediterranean loquat scab. Acta Horticulturae, 750, 343–347. es_ES
dc.description.references Sánchez-Torres, P., Hinarejos, R., & Tuset, J. J. (2009). Characterization and pathogenicity of Fusicladium eriobotryae, the fungal pathogen responsible for loquat scab. Plant Disease, 93, 1151–1157. es_ES
dc.description.references Schena, L., Li Destri Nicosia, M. G., Sanzani, S. M., Faedda, R., Ippolito, A., & Cacciola, S. O. (2013). Development of quantitative PCR detection methods for phytopathogenic fungi and oomycetes. Journal of Plant Pathology, 95, 7–24. es_ES
dc.description.references Scherm, H., Savelle, A. T., Boozer, R. T., & Foshee, W. G. (2008). Seasonal dynamics of conidial production potential of Fusicladium carpophilum on twig lesions in south eastern peach orchards. Plant Disease, 92, 47–50. es_ES
dc.description.references Schrader, C., Schielke, A., Ellerbroek, L., & Johne, R. (2012). PCR inhibitors – Occurrence, properties and removal. Journal of Applied Microbiology, 113, 1014–1026. es_ES
dc.description.references Schubert, K. S., Ritschel, A. R., & Braun, U. B. (2003). A monograph of Fusicladium s. lat. (Hyphomycetes). Schlechtendalia, 9, 1–132. es_ES
dc.description.references Soler, E., Martínez-Calvo, J., Llácer, G., & Badenes, M. L. (2007). Loquat in Spain: Production and marketing. Acta Horticulturae, 750, 45–47. es_ES
dc.description.references van Leeuwen, G. C. M., Holb, I. J., & Jeger, M. J. (2002). Factors affecting mummification and sporulation of pome fruit infected by Monilinia fructigena in Dutch orchards. Plant Pathology, 51, 787–793. es_ES
dc.description.references Villarino, M., Melgarejo, P., Usall, J., Segarra, J., & De Cal, A. (2010). Primary inoculum sources of Monilinia spp. in Spanish peach orchards and their relative importance in brown rot. Plant Disease, 94, 1048–1054. es_ES
dc.description.references Viruega, J. R., Moral, J., Roca, L. F., Navarro, N., & Trapero, A. (2013). Spilocaea oleagina in olive groves of southern Spain: Survival, inoculum production, and dispersal. Plant Disease, 97, 1549–1556. es_ES
dc.subject.ods 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible es_ES


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