Clara-Trujillo, S.; Marin-Paya, JC.; Cordón, L.; Sempere, A.; Gallego-Ferrer, G.; Gómez Ribelles, JL. (2019). Biomimetic microspheres for 3D mesenchymal stem cell culture and characterization. Colloids and Surfaces B Biointerfaces. 177:68-76. https://doi.org/10.1016/j.colsurfb.2019.01.050
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/156658
Title:
|
Biomimetic microspheres for 3D mesenchymal stem cell culture and characterization
|
Author:
|
Clara-Trujillo, Sandra
Marin-Paya, Juan Carlos
Cordón, Lourdes
Sempere, Amparo
Gallego-Ferrer, Gloria
Gómez Ribelles, José Luís
|
UPV Unit:
|
Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada
|
Issued date:
|
|
Abstract:
|
[EN] Stem cells reside in niches, specialized microenvironments that sustain and regulate their fate. Extracellular matrix (ECM), paracrine factors or other cells are key niche regulating elements. As the conventional 2D ...[+]
[EN] Stem cells reside in niches, specialized microenvironments that sustain and regulate their fate. Extracellular matrix (ECM), paracrine factors or other cells are key niche regulating elements. As the conventional 2D cell culture lacks these elements, it can alter the properties of naive stem cells. In this work we designed a novel biomimetic microenvironment for cell culture, consisting of magnetic microspheres, prepared with acrylates and acrylic acid copolymers and functionalized with fibronectin or hyaluronic acid as ECM coatings. To characterize cell proliferation and adhesion, porcine mesenchymal stem cells (MSCs) were grown with the different microspheres. The results showed that the 3D environments presented similar proliferation to the 2D culture and that fibronectin allows cell adhesion, while hyaluronic acid hinders it. In the 3D environments, cells reorganize the microspheres to grow in aggregates, highlighting the advantages of microspheres as 3D environments and allowing the cells to adapt the environment to their requirements.
[-]
|
Subjects:
|
3D environment
,
Extracellular matrix coatings
,
Microspheres
,
Mesenchymal stem cells
,
Surface modification
|
Copyrigths:
|
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
|
Source:
|
Colloids and Surfaces B Biointerfaces. (issn:
0927-7765
)
|
DOI:
|
10.1016/j.colsurfb.2019.01.050
|
Publisher:
|
Elsevier
|
Publisher version:
|
https://doi.org/10.1016/j.colsurfb.2019.01.050
|
Project ID:
|
info:eu-repo/grantAgreement/MINECO//CB16%2F12%2F00284/ES/CANCER/
info:eu-repo/grantAgreement/GVA//PROMETEO%2F2016%2F063/ES/MEDULA OSEA ARTIFICIAL PARA PERSONALIZAR EL TRATAMIENTO DE PACIENTES DE CANCERES DE SANGRE/
info:eu-repo/grantAgreement/MECD//FPU17%2F05810/
|
Thanks:
|
PROMETEO/2016/063 project is acknowledged. This work was
partially financed with FEDER funds (CIBERONC (CB16/12/00284)).
The CIBER-BBN initiative is funded by the VI National R&D&I Plan
2008-2011, Iniciativa Ingenio ...[+]
PROMETEO/2016/063 project is acknowledged. This work was
partially financed with FEDER funds (CIBERONC (CB16/12/00284)).
The CIBER-BBN initiative is funded by the VI National R&D&I Plan
2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions
are financed by the Instituto de Salud Carlos III with assistance from the
European Regional Development Fund. This work was also supported
by the Spanish Ministry of Science, Innovation and Universities trough
Sandra Clara-Trujillo FPU17/05810 grant.
[-]
|
Type:
|
Artículo
|