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Reassessment of Viroid RNA Cytosine Methylation Status at the Single Nucleotide Level

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Reassessment of Viroid RNA Cytosine Methylation Status at the Single Nucleotide Level

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dc.contributor.author Di Serio, Francesco es_ES
dc.contributor.author Torchetti, Enza Maria es_ES
dc.contributor.author Daròs, José-Antonio es_ES
dc.contributor.author Navarro, Beatriz es_ES
dc.date.accessioned 2021-01-09T04:32:20Z
dc.date.available 2021-01-09T04:32:20Z
dc.date.issued 2019-04-18 es_ES
dc.identifier.issn 1999-4915 es_ES
dc.identifier.uri http://hdl.handle.net/10251/158510
dc.description.abstract [EN] Composed of a few hundreds of nucleotides, viroids are infectious, circular, non-protein coding RNAs able to usurp plant cellular enzymes and molecular machineries to replicate and move in their hosts. Several secondary and tertiary RNA structural motifs have been implicated in the viroid infectious cycle, but whether modified nucleotides, such as 5C-methylcytosine (m(5)C), also play a role has not been deeply investigated so far. Here, the possible existence of m(5)C in both RNA polarity strands of potato spindle tuber viroid and avocado sunblotch viroid -which are representative members of the nucleus- and chloroplast-replicating viroids, respectively- has been assessed at single nucleotide level. We show that a standard bisulfite protocol efficiently used for identifying m(5)C in cellular RNAs may generate false positive results in the case of the highly structured viroid RNAs. Applying a bisulfite conversion protocol specifically adapted to RNAs with high secondary structure, no m(5)C was identified in both polarity strands of both viroids, indicating that this specific nucleotide modification does not likely play a role in viroid biology es_ES
dc.description.sponsorship This project was partially funded by CNR Short-Term Mobility Programs (2013 and 2014 to FDS) and by the Spanish Ministerio de Ciencia, Innovacion y Universidades grant BIO2017-83184-R (co-financed FEDER funds). es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Viruses es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Viroid RNA es_ES
dc.subject Bisulfite sequencing es_ES
dc.subject Nucleotide modification es_ES
dc.subject C5-methylcytosine es_ES
dc.title Reassessment of Viroid RNA Cytosine Methylation Status at the Single Nucleotide Level es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/v11040357 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83184-R/ES/VIRUS DE PLANTAS: PATOGENOS Y TAMBIEN VECTORES PARA LA PRODUCCION DE PROTEINAS, METABOLITOS, RNAS Y NANOPARTICULAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Di Serio, F.; Torchetti, EM.; Daròs, J.; Navarro, B. (2019). Reassessment of Viroid RNA Cytosine Methylation Status at the Single Nucleotide Level. Viruses. 11(4):1-9. https://doi.org/10.3390/v11040357 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/v11040357 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 9 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 11 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 31003406 es_ES
dc.identifier.pmcid PMC6521008 es_ES
dc.relation.pasarela S\406633 es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Consiglio Nazionale delle Ricerche, Italia es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
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