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dc.contributor.author | Ruiz-Ruiz, Susana | es_ES |
dc.contributor.author | Navarro, Beatriz | es_ES |
dc.contributor.author | PEÑA GARCIA, LEANDRO | es_ES |
dc.contributor.author | Navarro, Luis | es_ES |
dc.contributor.author | Moreno, Pedro | es_ES |
dc.contributor.author | Di Serio, Francesco | es_ES |
dc.contributor.author | FLORES PEDAUYE, RICARDO | es_ES |
dc.date.accessioned | 2021-01-20T04:32:25Z | |
dc.date.available | 2021-01-20T04:32:25Z | |
dc.date.issued | 2019 | es_ES |
dc.identifier.issn | 1064-3745 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/159537 | |
dc.description.abstract | [EN] To dissect the host RNA silencing response incited by citrus tristeza virus (CTV, genus Closterovirus), a (+) ssRNA of similar to 19300 nt, and the counter reaction deployed by the virus via its three RNA silencing suppressors (RSS), the small RNAs (sRNAs) of three virus-host combinations were deep sequenced. The subsequent analysis indicated that CTV sRNAs (1) constitute more than half of the total sRNAs in the susceptible Mexican lime and sweet orange, while only 3.5% in the restrictive sour orange; (2) are mostly of 21-22 nt, with those of (+) sense predominating slightly; and (3) derive from all the CTV genome, as evidenced by its entire recomposition from viral sRNA contigs but adopt an asymmetric pattern with a hotspot mapping at the 3'-terminal similar to 2500 nt. The citrus homologues of Arabidopsis Dicer-like (DCL) 4 and 2 most likely generate the 21 and 22 nt CTV sRNAs, respectively, by dicing the gRNA and the 3' co-terminal sgRNAs and, particularly, their double-stranded forms accumulating in infected cells. The plant sRNA profile, very similar and dominated by the 24 nt sRNAs in the three mock-inoculated controls, displayed a major reduction of the 24 nt sRNAs in Mexican lime and sweet orange, but not in sour orange. CTV infection also influences the levels of certain microRNAs. The high accumulation of CTV sRNAs in two of the citrus hosts examined suggests that it is not their synthesis, but their function, the target of the RSS encoded by CTV: p25 (intercellular), p23 (intracellular) and p20 (both). The two latter might block the loading of CTV sRNAs into the RNA silencing complex or interfere with it through alternative mechanisms. Of the three CTV RSS, p23 is the one that has been more thoroughly studied. It is a multifunctional RNA-binding protein with a putative Zn finger domain and basic motifs that (1) has no homologues in other closteroviruses, (2) accumulates in the nucleolus and plasmodesmata, (3) regulates the asymmetric balance of CTV (+) and (-) RNA strands, and (4) induces CTV syndromes and stimulates systemic infection in certain citrus species when expressed as a transgene ectopically or in phloem-associated cells. | es_ES |
dc.description.sponsorship | This research was supported by a grant (Prometeo/2008/121) from the Generalitat Valenciana, Spain, and by a grant (AGL2009-08052) from the Ministerio de Ciencia e Innovacio¿nFondo Europeo de Desarrollo Regional. S. Ruiz-Ruiz was additionally supported by a postdoctoral contract from the Generalitat Valenciana (APOSTD/2012/020, Program VALi+d). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Springer | es_ES |
dc.relation.ispartof | Methods in Molecular Biology | es_ES |
dc.relation.ispartof | Citrus Tristeza Virus: Methods and Protocols | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Closteroviruses | es_ES |
dc.subject | MicroRNAs | es_ES |
dc.subject | RNA silencing | es_ES |
dc.subject | Small interfering RNAs | es_ES |
dc.subject.classification | BIOQUIMICA Y BIOLOGIA MOLECULAR | es_ES |
dc.title | Citrus tristeza virus: Host RNA Silencing and Virus Counteraction | es_ES |
dc.type | Artículo | es_ES |
dc.type | Capítulo de libro | es_ES |
dc.identifier.doi | 10.1007/978-1-4939-9558-5_14 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Generalitat Valenciana//PROMETEO08%2F2008%2F121/ES/Biotecnología de cítricos/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//AGL2009-08052/ES/Mejora Genetica De La Calidad Y De La Respuesta A Estreses Bioticos De Los Citricos Mediante Ingenieria Genetica/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//APOSTD%2F2012%2F020/ | es_ES |
dc.rights.accessRights | Cerrado | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.description.bibliographicCitation | Ruiz-Ruiz, S.; Navarro, B.; Peña Garcia, L.; Navarro, L.; Moreno, P.; Di Serio, F.; Flores Pedauye, R. (2019). Citrus tristeza virus: Host RNA Silencing and Virus Counteraction. Methods in Molecular Biology. 2015:195-207. https://doi.org/10.1007/978-1-4939-9558-5_14 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1007/978-1-4939-9558-5_14 | es_ES |
dc.description.upvformatpinicio | 195 | es_ES |
dc.description.upvformatpfin | 207 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 2015 | es_ES |
dc.relation.pasarela | S\406700 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
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