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Expression and function of the bHLH genes ALCATRAZ and SPATULA in selected Solanaceae species

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Expression and function of the bHLH genes ALCATRAZ and SPATULA in selected Solanaceae species

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dc.contributor.author Ortiz-Ramirez, Clara Inés es_ES
dc.contributor.author Giraldo, Marco A. es_ES
dc.contributor.author FERRANDIZ MAESTRE, CRISTINA es_ES
dc.contributor.author Pabon-Mora, Natalia es_ES
dc.date.accessioned 2021-02-03T04:33:03Z
dc.date.available 2021-02-03T04:33:03Z
dc.date.issued 2019-08 es_ES
dc.identifier.issn 0960-7412 es_ES
dc.identifier.uri http://hdl.handle.net/10251/160587
dc.description.abstract [EN] The genetic mechanisms underlying fruit development have been identified in Arabidopsis and have been comparatively studied in tomato as a representative of fleshy fruits. However, comparative expression and functional analyses on the bHLH genes downstream the genetic network, ALCATRAZ (ALC) and SPATULA (SPT), which are involved in the formation of the dehiscence zone in Arabidopsis, have not been functionally studied in the Solanaceae. Here, we perform detailed expression and functional studies of ALC/SPT homologs in Nicotiana obtusifolia with capsules, and in Capsicum annuum and Solanum lycopersicum with berries. In Solanaceae, ALC and SPT genes are expressed in leaves, and all floral organs, especially in petal margins, stamens and carpels; however, their expression changes during fruit maturation according to the fruit type. Functional analyses show that downregulation of ALC/SPT genes does not have an effect on gynoecium patterning; however, they have acquired opposite roles in petal expansion and have been co-opted in leaf pigmentation in Solanaceae. In addition, ALC/SPT genes repress lignification in time and space during fruit development in Solanaceae. Altogether, some roles of ALC and SPT genes are different between Brassicaceae and Solanaceae; while the paralogs have undergone some subfunctionalization in the former they are mostly redundant in the latter. es_ES
dc.description.sponsorship This work was funded by COLCIENCIAS (111565842812), the iCOOP + 2016 COOPB20250 from the Centro Superior de Investigación Científica, CSIC, the ExpoSeed (H2020.MSCA-RISE-2015-691109) EU grant, the Convocatoria Programáticas 2017-16302, and the Estrategia de Sostenibilidad 2018-2019, from the Universidad de Antioquia. The authors would like to thank the group members of the Ferrándiz and Madueño Labs at IBMCP-UPV for training and help in the standardization of in situ hybridization. Finally, the authors thank Ricardo Callejas and Zulma Monsalve, from the Universidad de Antioquia, for their helpful suggestions during this research. es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation.ispartof The Plant Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject ALCATRAZ es_ES
dc.subject Berries es_ES
dc.subject Capsules es_ES
dc.subject Fruit development es_ES
dc.subject Lignification es_ES
dc.subject Pigmentation es_ES
dc.subject SPATULA es_ES
dc.subject Solanaceae es_ES
dc.title Expression and function of the bHLH genes ALCATRAZ and SPATULA in selected Solanaceae species es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/tpj.14352 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/691109/EU/Exploring the molecular control of seed yield in crops/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/COLCIENCIAS//111565842812/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//FJCI-2014-20250/ES/FJCI-2014-20250/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UdeA//2017-16302/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Ortiz-Ramirez, CI.; Giraldo, MA.; Ferrandiz Maestre, C.; Pabon-Mora, N. (2019). Expression and function of the bHLH genes ALCATRAZ and SPATULA in selected Solanaceae species. The Plant Journal. 99(4):686-702. https://doi.org/10.1111/tpj.14352 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/tpj.14352 es_ES
dc.description.upvformatpinicio 686 es_ES
dc.description.upvformatpfin 702 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 99 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 31009131 es_ES
dc.relation.pasarela S\401537 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Universidad de Antioquia es_ES
dc.contributor.funder Departamento Administrativo de Ciencia, Tecnología e Innovación, Colombia es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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