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Argonaute PIWI domain and microRNA duplex structure contribute to small RNA sorting in Arabidopsis.

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Argonaute PIWI domain and microRNA duplex structure contribute to small RNA sorting in Arabidopsis.

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dc.contributor.author Zhang, Xiaoming es_ES
dc.contributor.author Niu, DongDong es_ES
dc.contributor.author CARBONELL, ALBERTO es_ES
dc.contributor.author Wang, Airong es_ES
dc.contributor.author Lee, Angel es_ES
dc.contributor.author Tun, Vinnary es_ES
dc.contributor.author Wang, Zonghua es_ES
dc.contributor.author Carrington, James C. es_ES
dc.contributor.author Chang, Chia-en A. es_ES
dc.contributor.author Jin, Hailing es_ES
dc.date.accessioned 2021-02-11T04:32:20Z
dc.date.available 2021-02-11T04:32:20Z
dc.date.issued 2014-11-16 es_ES
dc.identifier.issn 2041-1723 es_ES
dc.identifier.uri http://hdl.handle.net/10251/161051
dc.description.abstract [EN] Small RNAs (sRNAs) are loaded into ARGONAUTE (AGO) proteins to induce gene silencing. In plants, the 5¿-terminal nucleotide is important for sRNA sorting into different AGOs. Here we show that microRNA (miRNA) duplex structure also contributes to miRNA sorting. Base pairing at the 15th nucleotide of a miRNA duplex is important for miRNA sorting in both Arabidopsis ¿AGO1 and ¿AGO2. ¿AGO2 favours miRNA duplexes with no middle mismatches, whereas ¿AGO1 tolerates, or prefers, duplexes with central mismatches. AGO structure modelling and mutational analyses reveal that the QF-V motif within the conserved PIWI domain contributes to recognition of base pairing at the 15th nucleotide of a duplex, while the DDDE catalytic core of ¿AtAGO2 is important for recognition of the central nucleotides. Finally, we rescued the adaxialized phenotype of ¿ago1-12, which is largely due to miR165 loss-of-function, by changing miR165 duplex structure which we predict redirects it to ¿AGO2. es_ES
dc.description.sponsorship We thank Yifan E. Lii, Patricia Springer and Hongwei Zhao for helpful comments; Yijun Qi, Xuemei Chen, David Baulcombe, Olivier Voinnet and Robert A. Martienssen for providing antibodies, constructs and seeds. This work was supported by an NIH (R01 GM093008), an NSF Career Award (MCB-0642843), an NSF Award (IOS-1257576) and an AES-CE Award (PPA-7517H) to H.J., and by NIH (AI043288) and NSF (MCB-0956526, MCB-1231726) grants to J.C.C. We thank Rizi Ai and Wanli You for constructing the protein and RNA complex structures es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Nature Communications es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject RNA silencing es_ES
dc.subject Argonaute es_ES
dc.subject Small RNA es_ES
dc.subject MicroRNA es_ES
dc.title Argonaute PIWI domain and microRNA duplex structure contribute to small RNA sorting in Arabidopsis. es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/ncomms6468 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//0956526/US/Function of Arabidopsis Small RNA-ARGONAUTE Complexes/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//0642843/US/CAREER:Genome-wide Analysis of Pathogen-induced Endogenous siRNAs in Plant Defense Responses in Arabidopsis/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//R01GM093008/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//AI043288/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//1257576/US/Investigate the role of small RNAs of a necrotrophic fungal pathogen B. cinerea in suppressing host Immunity/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//1231726/US/Function of Arabidopsis Small RNA-ARGONAUTE Complexes/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Zhang, X.; Niu, D.; Carbonell, A.; Wang, A.; Lee, A.; Tun, V.; Wang, Z.... (2014). Argonaute PIWI domain and microRNA duplex structure contribute to small RNA sorting in Arabidopsis. Nature Communications. 5:1-11. https://doi.org/10.1038/ncomms6468 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/ncomms6468 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 11 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 5 es_ES
dc.identifier.pmid 25406978 es_ES
dc.identifier.pmcid PMC4238042 es_ES
dc.relation.pasarela S\378022 es_ES
dc.contributor.funder National Science Foundation, EEUU es_ES
dc.contributor.funder National Institutes of Health, EEUU es_ES
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