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The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner

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The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner

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dc.contributor.author CARBONELL, ALBERTO es_ES
dc.contributor.author Dujovny, Gabriela es_ES
dc.contributor.author Garcia, Juan Antonio es_ES
dc.contributor.author Valli, Adrian es_ES
dc.date.accessioned 2021-02-12T04:31:08Z
dc.date.available 2021-02-12T04:31:08Z
dc.date.issued 2012-02 es_ES
dc.identifier.issn 0894-0282 es_ES
dc.identifier.uri http://hdl.handle.net/10251/161157
dc.description.abstract [EN] Plant viruses of the genera Potyvirus and Ipomovirus (Potyviridae family) use unrelated RNA silencing suppressors (RSS) to counteract antiviral RNA silencing responses. HCPro is the RSS of Potyvirus spp., and its activity is enhanced by the upstream P1 protein. Distinctively, the ipomovirus Cucumber vein yellowing virus (CVYV) lacks HCPro but contains two P1 copies in tandem (P1aP1b), the second of which functions as RSS. Using chimeras based on the potyvirus Plum pox virus (PPV), we found that P1b can functionally replace HCPro in potyviral infections of Nicotiana plants. Interestingly, P1a, the CVYV protein homologous to potyviral P1, disrupted the silencing suppression activity of P1b and reduced the infection efficiency of PPV in Nicotiana benthamiana. Testing the influence of RSS in host specificity, we found that a P1b-expressing chimera poorly infected PPV's natural host, Prunus persica. Conversely, P1b conferred on PPV chimeras the ability to replicate locally in cucumber, CVYV's natural host. The deleterious effect of P1a on PPV infection is host dependent, because the P1aP1b-expressing PPV chimera accumulated in cucumber to higher levels than PPV expressing P1b alone. These results demonstrate that a potyvirus can use different RSS, and that particular RSS and upstream P1-like proteins contribute to defining the virus host range. es_ES
dc.description.sponsorship We thank H. Garcia-Ruiz for critical reading of the manuscript, D. Baulcombe for providing GFP and P19 expression vectors, Rijk Zwaan Iberica (Almeria, Spain) for supplying cucumber seeds, and E. Dominguez for technical assistance. This work was supported by grants BIO2010-18541 from Spanish MICINN, SAL/0185/2006 from Comunidad de Madrid, and KBBE-204429 from European Union. A. Valli was a recipient of an I3P fellowship from CSIC-Fondo Social Europa) es_ES
dc.language Inglés es_ES
dc.publisher Scientific Societies es_ES
dc.relation info:eu-repo/grantAgreement/MICINN//BIO2010-18541/ES/FACTORES DE LA INTERACCION PLANTA-VIRUS RELEVANTES PARA EL CONTROL DEL VIRUS DE LA SHARKA Y PARA SU USO COMO HERRAMIENTA BIOTECNOLOGICA/ es_ES
dc.relation CAM/SAL/0185/2006 es_ES
dc.relation EC/KBBE-204429 es_ES
dc.relation.ispartof Molecular Plant-Microbe Interactions es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Ppv es_ES
dc.subject RNA silencing es_ES
dc.subject Suppressors es_ES
dc.title The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1094/MPMI-08-11-0216 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Carbonell, A.; Dujovny, G.; Garcia, JA.; Valli, A. (2012). The Cucumber vein yellowing virus silencing suppressor P1b can functionally replace HCPro in Plum pox virus infection in a host-specific manner. Molecular Plant-Microbe Interactions. 25(2):151-164. https://doi.org/10.1094/MPMI-08-11-0216 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1094/MPMI-08-11-0216 es_ES
dc.description.upvformatpinicio 151 es_ES
dc.description.upvformatpfin 164 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 25 es_ES
dc.description.issue 2 es_ES
dc.identifier.pmid 21970691 es_ES
dc.relation.pasarela S\378013 es_ES
dc.contributor.funder Comunidad de Madrid es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Ministerio de Educación y Ciencia e Innovación es_ES


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