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dc.contributor.author | Maliogka, Varvara I. | es_ES |
dc.contributor.author | Calvo, Maria | es_ES |
dc.contributor.author | CARBONELL, ALBERTO | es_ES |
dc.contributor.author | Garcia, Juan Antonio | es_ES |
dc.contributor.author | Valli, Adrian | es_ES |
dc.date.accessioned | 2021-02-12T04:31:12Z | |
dc.date.available | 2021-02-12T04:31:12Z | |
dc.date.issued | 2012-07 | es_ES |
dc.identifier.issn | 0022-1317 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/161159 | |
dc.description.abstract | [EN] HCPro, the RNA silencing suppressor (RSS) of viruses belonging to the Potyvirus genus in the Potyviridae family, is a multifunctional protein presumably involved in all essential steps of the viral infection cycle. Recent studies have shown that Plum pox potyvirus (PPV) HCPro can be successfully replaced by Cucumber vein yellowing ipomovirus P1b, a sequence unrelated RSS from a virus of the same family. In order to gain insight into the requirement of a particular RSS to establish a successful potyviral infection, we tested the ability of different heterologous RSSs from both plant- and animal-infecting viruses to substitute HCPro. Making use of engineered PPV chimeras, we show that PPV HCPro can be functionally replaced by some, but not all, unrelated RSSs, including the NS1 protein of the mammalian-infecting Influenza A virus. Interestingly, the capacity of a particular RSS to replace HCPro does not strictly correlate with its RNA silencing suppression strength. Altogether, our results suggest that not all suppression strategies are equally suitable for an efficient escape of PPV from the RNA silencing machinery. The approach followed here based on using PPV chimeras in which an under-consideration RSS substitutes for HCPro could further help to study the function of diverse RSSs in a ¿highly-sensitive¿ RNA silencing context, such as that taking place in plant cells during the process of a viral infection. | es_ES |
dc.description.sponsorship | We are especially grateful to those people who sent us plasmids containing the DNA sequence of different viral proteins. We thank Veronique Ziegler-Graff for providing BWYV P0, Ana Giner and Juan Jose Lopez-Moya for providing SPMMV P1, Joel Milner for providing CaMV P6, Maria Rosa Lopez-Huertas and Jose Alcami for providing HIV Tat, Jan Kreuze for providing SPCSV RNase3, and M. Taliansky for providing GRV ORF3. We thank Herman Scholthof and Ariel Rodriguez for providing anti-P19 and anti-NS1 serum, respectively. We are also grateful to David Baulcombe for providing the GFP expression vector and TBSV P19-containing plasmid, and Mark Curtis for providing the pMDC32 destination vector. This work was supported by grants from Spanish MICINN (BIO2010-18541) and the European Union (KBBE-204429). M. C. was the recipient of an 13P fellowship from CSIC-Fondo Social Europeo. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Microbiology Society | es_ES |
dc.relation.ispartof | Journal of General Virology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | RNA silencing | es_ES |
dc.subject | Virus | es_ES |
dc.subject | PPV | es_ES |
dc.subject | Suppressors | es_ES |
dc.title | Heterologous RNA silencing suppressors from both plant- and animal-infecting viruses support Plum pox virus infection | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1099/vir.0.042168-0 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BIO2010-18541/ES/FACTORES DE LA INTERACCION PLANTA-VIRUS RELEVANTES PARA EL CONTROL DEL VIRUS DE LA SHARKA Y PARA SU USO COMO HERRAMIENTA BIOTECNOLOGICA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/204429/EU/Sharka Containment/ | |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Maliogka, VI.; Calvo, M.; Carbonell, A.; Garcia, JA.; Valli, A. (2012). Heterologous RNA silencing suppressors from both plant- and animal-infecting viruses support Plum pox virus infection. Journal of General Virology. 93(7):1601-1611. https://doi.org/10.1099/vir.0.042168-0 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1099/vir.0.042168-0 | es_ES |
dc.description.upvformatpinicio | 1601 | es_ES |
dc.description.upvformatpfin | 1611 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 93 | es_ES |
dc.description.issue | 7 | es_ES |
dc.identifier.pmid | 22513385 | es_ES |
dc.relation.pasarela | S\378010 | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
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