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P-SAMS: a web suite for plant artificial microRNA and synthetic trans-acting small interfering RNA design

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P-SAMS: a web suite for plant artificial microRNA and synthetic trans-acting small interfering RNA design

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dc.contributor.author Fahlgren, Noah es_ES
dc.contributor.author Hill, Steven T. es_ES
dc.contributor.author Carrington, James C. es_ES
dc.contributor.author CARBONELL, ALBERTO es_ES
dc.date.accessioned 2021-02-13T04:31:42Z
dc.date.available 2021-02-13T04:31:42Z
dc.date.issued 2016-01-01 es_ES
dc.identifier.issn 1367-4803 es_ES
dc.identifier.uri http://hdl.handle.net/10251/161198
dc.description.abstract [EN] The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syntasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into `B/c¿ compatible vectors. es_ES
dc.description.sponsorship This work was supported by the National Institutes of Health [grant number AI043288 to J.C.C.]; the National Science Foundation [grants numbers MCB-1231726, MCB-1330562 to J.C.C.]; and the United States Department of Agriculture [fellowship number MOW-2012-01361 to N.F.). es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Bioinformatics es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Artificial microRNA es_ES
dc.subject Syn.tasiRNA es_ES
dc.subject Design, Web tool es_ES
dc.title P-SAMS: a web suite for plant artificial microRNA and synthetic trans-acting small interfering RNA design es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/bioinformatics/btv534 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//1231726/US/Function of Arabidopsis Small RNA-ARGONAUTE Complexes/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//AI043288/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//1330562/US/Integration of developmental signals by plant ARGONAUTES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/USDA//MOW-2012-01361/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Fahlgren, N.; Hill, ST.; Carrington, JC.; Carbonell, A. (2016). P-SAMS: a web suite for plant artificial microRNA and synthetic trans-acting small interfering RNA design. Bioinformatics. 32(1):157-158. https://doi.org/10.1093/bioinformatics/btv534 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/bioinformatics/btv534 es_ES
dc.description.upvformatpinicio 157 es_ES
dc.description.upvformatpfin 158 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 32 es_ES
dc.description.issue 1 es_ES
dc.identifier.pmid 26382195 es_ES
dc.identifier.pmcid PMC4681993 es_ES
dc.relation.pasarela S\378038 es_ES
dc.contributor.funder National Institutes of Health, EEUU
dc.contributor.funder National Science Foundation, EEUU
dc.contributor.funder United States Department of Agriculture, Agricultural Research Service
dc.contributor.funder U.S. Department of Agriculture es_ES
dc.description.references Ahmed, F., Dai, X., & Zhao, P. X. (2015). Bioinformatics Tools for Achieving Better Gene Silencing in Plants. Plant Gene Silencing, 43-60. doi:10.1007/978-1-4939-2453-0_3 es_ES
dc.description.references Carbonell, A., Takeda, A., Fahlgren, N., Johnson, S. C., Cuperus, J. T., & Carrington, J. C. (2014). New Generation of Artificial MicroRNA and Synthetic Trans-Acting Small Interfering RNA Vectors for Efficient Gene Silencing in Arabidopsis. Plant Physiology, 165(1), 15-29. doi:10.1104/pp.113.234989 es_ES
dc.description.references Carbonell, A., Fahlgren, N., Mitchell, S., Cox, K. L., Reilly, K. C., Mockler, T. C., & Carrington, J. C. (2015). Highly specific gene silencing in a monocot species by artificial micro RNA s derived from chimeric mi RNA precursors. The Plant Journal, 82(6), 1061-1075. doi:10.1111/tpj.12835 es_ES
dc.description.references Fahlgren, N., & Carrington, J. C. (2009). miRNA Target Prediction in Plants. Plant MicroRNAs, 51-57. doi:10.1007/978-1-60327-005-2_4 es_ES
dc.description.references Ossowski, S., Schwab, R., & Weigel, D. (2008). Gene silencing in plants using artificial microRNAs and other small RNAs. The Plant Journal, 53(4), 674-690. doi:10.1111/j.1365-313x.2007.03328.x es_ES
dc.description.references Schwab, R., Ossowski, S., Riester, M., Warthmann, N., & Weigel, D. (2006). Highly Specific Gene Silencing by Artificial MicroRNAs inArabidopsis. The Plant Cell, 18(5), 1121-1133. doi:10.1105/tpc.105.039834 es_ES
dc.description.references Tiwari, M., Sharma, D., & Trivedi, P. K. (2014). Artificial microRNA mediated gene silencing in plants: progress and perspectives. Plant Molecular Biology, 86(1-2), 1-18. doi:10.1007/s11103-014-0224-7 es_ES
dc.description.references Zhang, Z. J. (2014). Artificial trans-acting small interfering RNA: a tool for plant biology study and crop improvements. Planta, 239(6), 1139-1146. doi:10.1007/s00425-014-2054-x es_ES


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