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Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection

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Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection

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dc.contributor.author Garcia-Ruiz, Hernan es_ES
dc.contributor.author CARBONELL, ALBERTO es_ES
dc.contributor.author Hoyer, J. Steen es_ES
dc.contributor.author Fahlgren, Noah es_ES
dc.contributor.author Gilbert, Kerrigan B. es_ES
dc.contributor.author Takeda, Atsushi es_ES
dc.contributor.author Giampetruzzi, Annalisa es_ES
dc.contributor.author García Ruíz, Maira T. es_ES
dc.contributor.author McGinn, Michaela G. es_ES
dc.contributor.author Lowery, Nicholas es_ES
dc.contributor.author Martínez Baladejo, María T. es_ES
dc.contributor.author Carrington, James C. es_ES
dc.date.accessioned 2021-02-13T04:31:58Z
dc.date.available 2021-02-13T04:31:58Z
dc.date.issued 2015-03 es_ES
dc.identifier.issn 1553-7366 es_ES
dc.identifier.uri http://hdl.handle.net/10251/161205
dc.description.abstract [EN] In eukaryotes, ARGONAUTE proteins (AGOs) associate with microRNAs (miRNAs), short interfering RNAs (siRNAs), and other classes of small RNAs to regulate target RNA or target loci. Viral infection in plants induces a potent and highly specific antiviral RNA silencing response characterized by the formation of virus-derived siRNAs. Arabidopsis thaliana has ten AGO genes of which AGO1, AGO2, and AGO7 have been shown to play roles in antiviral defense. A genetic analysis was used to identify and characterize the roles of AGO proteins in antiviral defense against Turnip mosaic virus (TuMV) in Arabidopsis. AGO1, AGO2 and AGO10 promoted anti-TuMV defense in a modular way in various organs, with AGO2 providing a prominent antiviral role in leaves. AGO5, AGO7 and AGO10 had minor effects in leaves. AGO1 and AGO10 had overlapping antiviral functions in inflorescence tissues after systemic movement of the virus, although the roles of AGO1 and AGO10 accounted for only a minor amount of the overall antiviral activity. By combining AGO protein immunoprecipitation with high-throughput sequencing of associated small RNAs, AGO2, AGO10, and to a lesser extent AGO1 were shown to associate with siRNAs derived from silencing suppressor (HC-Pro)-deficient TuMV-AS9, but not with siRNAs derived from wild-type TuMV. Co-immunoprecipitation and small RNA sequencing revealed that viral siRNAs broadly associated with wild-type HC-Pro during TuMV infection. These results support the hypothesis that suppression of antiviral silencing during TuMV infection, at least in part, occurs through sequestration of virus-derived siRNAs away from antiviral AGO proteins by HC-Pro. These findings indicate that distinct AGO proteins function as antiviral modules, and provide a molecular explanation for the silencing suppressor activity of HC-Pro. es_ES
dc.description.sponsorship National Institutes of Health (www.nih.gov) grant AI43288 to JCC. National Science Foundation (www.nsf.gov) grant MCB-0956526 to JCC. Helen Hay Whitney (www.hhwf.org) Post-Doctoral fellowship (F-972) to HGR. USDA AFRI NIFA (www.csrees.usda.gov) Postdoctoral Fellowship (MOW-2012-01361) to NF. NSF (www.nsf.gov) Graduate Research Fellowship (DGE-1143954) to JSH Japan Society for the Promotion of Science (www.jsps.go.jp) Postdoctoral Fellowship to AT. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. es_ES
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS Pathogens es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Antiviral silencing es_ES
dc.subject Argonautes es_ES
dc.subject Silencing suppressors es_ES
dc.title Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.ppat.1004755 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//0956526/US/Function of Arabidopsis Small RNA-ARGONAUTE Complexes/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIH//AI043288/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//1143954/US/Graduate Reserach Fellowship Program (GRFP)/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/HHWF//F-972/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NIFA//MOW-2012-01361/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Garcia-Ruiz, H.; Carbonell, A.; Hoyer, JS.; Fahlgren, N.; Gilbert, KB.; Takeda, A.; Giampetruzzi, A.... (2015). Roles and Programming of Arabidopsis ARGONAUTE Proteins during Turnip Mosaic Virus Infection. PLoS Pathogens. 11(3). https://doi.org/10.1371/journal.ppat.1004755 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1371/journal.ppat.1004755 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 11 es_ES
dc.description.issue 3 es_ES
dc.identifier.pmid 25806948 es_ES
dc.identifier.pmcid PMC4373807 es_ES
dc.relation.pasarela S\378032 es_ES
dc.contributor.funder Helen Hay Whitney Foundation es_ES
dc.contributor.funder National Science Foundation, EEUU es_ES
dc.contributor.funder National Institutes of Health, EEUU es_ES
dc.contributor.funder National Institute of Food and Agriculture, EEUU es_ES
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