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Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

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Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

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dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Bäuerl, Christine es_ES
dc.contributor.author Tomas-Vidal, A. es_ES
dc.contributor.author Jover Cerda, Miguel es_ES
dc.contributor.author Estruch, Guillem es_ES
dc.contributor.author Pérez Martínez, Gaspar es_ES
dc.contributor.author Martínez-Llorens, Silvia es_ES
dc.date.accessioned 2021-02-16T04:32:05Z
dc.date.available 2021-02-16T04:32:05Z
dc.date.issued 2020-10 es_ES
dc.identifier.uri http://hdl.handle.net/10251/161380
dc.description.abstract [EN] The interaction between diet and intestinal health has been widely discussed, although in vivo approaches have reported limitations. The intestine explant culture system developed provides an advantage since it reduces the number of experimental fish and increases the time of incubation compared to similar methods, becoming a valuable tool in the study of the interactions between pathogenic bacteria, rearing conditions, or dietary components and fish gut immune response. The objective of this study was to determine the influence of the total substitution of fish meal by plants on the immune intestinal status of seabream using an ex vivo bacterial challenge. For this aim, two growth stages of fish were assayed (12 g): phase I (90 days), up to 68 g, and phase II (305 days), up to 250 g. Additionally, in phase II, the effects of long term and short term exposure (15 days) to a plant protein (PP) diet were determined. PP diet altered the mucosal immune homeostasis, the younger fish being more sensitive, and the intestine from fish fed short-term plant diets showed a higher immune response than with long-term feeding. Vibrio alginolyticus (V. alginolyticus) triggered the highest immune and inflammatory response, while COX-2 expression was significantly induced by Photobacterium damselae subsp. Piscicida (P. damselae subsp. Piscicida), showing a positive high correlation between the pro-inflammatory genes encoding interleukin 1 beta (IL1-beta), interleukin 6 (IL-6) and cyclooxygenase 2(COX-2). es_ES
dc.description.sponsorship The research was supported by a grant financed by the Spanish Ministerio de Economia y Competitividad AGL2015-70487-P. and Generalitat Valenciana, IDIFEDER/2020/029 The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. It was additionally granted by Contrato Pre-doctoral para la Formacion de Profesorado Universitario from Subprogramas de Formacion y Movilidad within the Programa Estatal de Promocion del Talento y su Empleabilidad of the Ministerio de Educacion, Cultura y Deporte of Spain. es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof International Journal of Molecular Sciences es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Gilthead seabream es_ES
dc.subject Ex vivo es_ES
dc.subject Intestine explants culture es_ES
dc.subject RT-qPCR es_ES
dc.subject Inflammation es_ES
dc.subject Plant protein es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/ijms21207584 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2015-70487-P/ES/EXPLOTACION DE LOS MECANISMOS DE COMUNICACION BIDIRECCIONAL MICROBIOTA %2FHUESPED EN EL INTESTINO PARA EL DESARROLLO DE NUEVAS ESTRATEGIAS DIETETICAS CON PROBIOTICOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MECD//FPU13%2F01278/ES/FPU13%2F01278/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//IDIFEDER%2F2020%2F029/ES/ADQUISICIÓN DE UN CROMATÓGRAFO UHPLC: HACIA UNA PRODUCCIÓN ANIMAL ECOLÓGICA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Peñaranda, D.; Bäuerl, C.; Tomas-Vidal, A.; Jover Cerda, M.; Estruch, G.; Pérez Martínez, G.; Martínez-Llorens, S. (2020). Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet. International Journal of Molecular Sciences. 21(20):1-20. https://doi.org/10.3390/ijms21207584 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/ijms21207584 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 20 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 21 es_ES
dc.description.issue 20 es_ES
dc.identifier.eissn 1422-0067 es_ES
dc.identifier.pmid 33066515 es_ES
dc.identifier.pmcid PMC7588912 es_ES
dc.relation.pasarela S\419273 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Ministerio de Educación, Cultura y Deporte es_ES
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dc.subject.ods 09.- Desarrollar infraestructuras resilientes, promover la industrialización inclusiva y sostenible, y fomentar la innovación es_ES
dc.subject.ods 14.- Conservar y utilizar de forma sostenible los océanos, mares y recursos marinos para lograr el desarrollo sostenible es_ES


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