- -

Evaluation of different culturemedia for detection and quantification of H. pylori in environmental and clinical samples

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

Evaluation of different culturemedia for detection and quantification of H. pylori in environmental and clinical samples

Mostrar el registro sencillo del ítem

Ficheros en el ítem

Thumbnail
Nombre: Hortelano;Moreno;Vesga ...
Tamaño: 870.1Kb
Formato: PDF
Descripción: Versión del Autor.
Abrir
[Cerrado]
Nombre: Hortelano2020_Art ...
Tamaño: 301.2Kb
Formato: PDF
Descripción: Versión editorial
Solicitar una copia al autor
dc.contributor.author Hortelano, Irene es_ES
dc.contributor.author Moreno Trigos, Mª Yolanda es_ES
dc.contributor.author Vesga, Fidson Juarismi es_ES
dc.contributor.author Ferrús Pérez, Mª Antonia es_ES
dc.date.accessioned 2021-02-19T04:33:59Z
dc.date.available 2021-02-19T04:33:59Z
dc.date.issued 2020-11 es_ES
dc.identifier.issn 1139-6709 es_ES
dc.identifier.uri http://hdl.handle.net/10251/161856
dc.description.abstract [EN] The objective of the present study was to establish the most suitable culture medium for the isolation ofH. pylorifrom environmental and clinical samples. Ten different culture media were compared and evaluated. Four of them had been previously described and were modified in this study. The rest of the media were designed de novo. Three different matrices, tap water, wastewater, and feces, were inoculated with serial dilutions ofH. pyloriNCTC 11637 strain at a final concentration of 10(4)and 10(3) CFU/ml and the recovery rates were calculated. From inoculated tap water and wastewater samples,H. pyloricolonies were recovered from four out of the analyzed culture media. When fecal samples were analyzed, the isolation of the pathogen under study was only possible from two culture media. Different optimal media were observed for each type of sample, even for wastewater and stool samples. Nevertheless, our results indicated that the combination of Dent Agar with polymyxin B sulfate did not inhibit the growth ofH. pyloriand was highly selective for its recovery, regardless of the sample origin. Thus, we propose the use of this medium as a diagnostic tool for the isolation ofH. pylorifrom environmental and clinical samples, as well as for epidemiological studies. es_ES
dc.description.sponsorship This work was supported by the Conselleria de Educacion, Investigacion, Cultura y Deporte, of the Community of Valencia, Spain, under project AICO/2018/273, and by the Spanish Ministry of Economy and Competitiveness AGL2014/53875-R Grant. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof International Microbiology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject H. pylori es_ES
dc.subject Isolation es_ES
dc.subject Identification es_ES
dc.subject Culture media es_ES
dc.subject Feces es_ES
dc.subject Environmental samples es_ES
dc.subject.classification MICROBIOLOGIA es_ES
dc.title Evaluation of different culturemedia for detection and quantification of H. pylori in environmental and clinical samples es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10123-020-00135-z es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2014-53875-R/ES/H. PYLORI Y OTROS HELICOBACTERS PATOGENOS EN AGUAS Y ALIMENTOS: DESARROLLO Y APLICACION DE HERRAMIENTAS MOLECULARES DIRIGIDAS A LA EVALUACION DEL RIESGO PARA EL CONSUMIDOR/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//AICO%2F2018%2F273/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Hortelano, I.; Moreno Trigos, MY.; Vesga, FJ.; Ferrús Pérez, MA. (2020). Evaluation of different culturemedia for detection and quantification of H. pylori in environmental and clinical samples. International Microbiology. 23(4):481-487. https://doi.org/10.1007/s10123-020-00135-z es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s10123-020-00135-z es_ES
dc.description.upvformatpinicio 481 es_ES
dc.description.upvformatpfin 487 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 23 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 32607781 es_ES
dc.relation.pasarela S\415175 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía y Empresa es_ES
dc.description.references Adams BL, Bates TC, Oliver JD (2003) Survival of Helicobacter pylori in a natural freshwater environment. Appl Environ Microbiol 69:7461–7466. https://doi.org/10.1128/aem.69.12.7462-7466.2003 es_ES
dc.description.references Al-Sulami AA, Al-Edani TA, Al-Abdulaz AA (2012) Culture method and PCR for the detection of Helicobacter pylori in drinking water in Basrah governorate Iraq. Gastroenterol Res Pract 12:5–5. https://doi.org/10.1155/2012/245167 es_ES
dc.description.references Álvarez-Ruiz R, Cuñat A, Picó Y (2018) Pollution fingerprinting of sewage sludge and their related risk in Albufera's wetland, Valencia. 16th IWA International conference on wetland Systems for Water Pollution Control. http://hdl.handle.net/10261/184328 es_ES
dc.description.references Ansorg R, Von Recklinghausen G, Pomarius R, Schmid EN (1991) Evaluation of techniques for isolation, subcultivation and preservation of Helicobacter pylori. J Clin Microbiol 29:51–53 es_ES
dc.description.references Atapoor S, Safarpoor Dehkordi F, Rahini E (2014) Detection of Helicobacter pylori in various types of vegetables and salads. Jundishapur J Microbiol. 7(5):e10013. https://doi.org/10.5812/jjm.10013 es_ES
dc.description.references Azevedo NF, Pacheco AP, Keevil CW, Vieira MJ (2004) Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water. Appl Environ Microbiol 40:490–493. https://doi.org/10.1128/AEM.70.1.490-493.2004 es_ES
dc.description.references Azevedo NF, Almedia L, Cerquerias S, Dias C, Keevil W, Vieira MJ (2007) Coccoid form of Helicobacter pylori as a morphological manifestation of cell adaptation to the environment. Appl Environ Microbiol 73(10):3423–3427. https://doi.org/10.1128/AEM.00047-07 es_ES
dc.description.references Bahrami AR, Rahimi E, Ghasemian Safaei H (2013) Detection of Helicobacter pylori in City water, dental units’ water, and bottled mineral water in Isfahan, Iran. Sci world J 5. https://doi.org/10.1128/AEM.00827-0810.1155/2013/280510 es_ES
dc.description.references Bai X, Xi C, Wu J (2016) Survival of Helicobacter pylori in the wastewater treatment process and the receiving river in Michigan, USA. J Water Health 14:692–698. https://doi.org/10.2166/wh.2016.259 es_ES
dc.description.references Bode G, Mauch F, Malfertheiner P (1993) The coccoid forms of Helicobacter pylori. Criteria for their viability. Epidemiol Infect 111:483–490. https://doi.org/10.1017/S0950268800057216 es_ES
dc.description.references Boro S, Sarma M, Sarma P (2016). Helicobacter pylori and Steps for its Elimination: A Review Global J Med Res (F) 16: 31–36 es_ES
dc.description.references Carbone M, Maugeri TL, Gugliandolo C, La Camera E, Biondo C, Fera MT (2005) Occurrence of Helicobacter pylori DNA in coastal environment of southern Italy (straits of Messina). J Appl Microbiol 98:768–774. https://doi.org/10.1111/j.1365-2672.2004.02517.x es_ES
dc.description.references Cellini L (2014) Helicobacter pylori: a chameleon-like approach to life. World J Gastroenterol 20:5575–5582. https://doi.org/10.3748/wjg.v20.i19.5575 es_ES
dc.description.references Degnan AJ, Sonzogni WC, Standridge JH (2003) Development of a plating medium for selection of Helicobacter pylori from water samples. Appl Environ Microbiol 69:2914–2918. https://doi.org/10.1128/AEM.69.5.2914-2918.2003 es_ES
dc.description.references Dent JC, McNulty CA (1988) Evaluation of a new selective medium for Campylobacter pylori. Eur J Clin Microbiol Infect Dis 7:555–558. https://doi.org/10.1007/bf01962615 es_ES
dc.description.references Duque-Jamaica R, Arévalo-Galvis A, Poutou-Piñales RA, Trespalacios AA (2010) Sequential statistical improvement of the liquid cultivation of Helicobacter pylori. Helicobacter 15:303–312. https://doi.org/10.1111/j.1523-5378.2010.00763.x es_ES
dc.description.references Fernández M, Contreras M, Suárez P, García-Amado MA (2007) Use of HP selective medium to detect Helicobacter pylori associated with other enteric bacteria in seawater and marine molluscs. Let Appl Microbiol 45:213–218. https://doi.org/10.1111/j.1472-765X.2007.02174.x es_ES
dc.description.references Giao MS, Azevedo NF, Wilks SA, Vieira MJ, Keevil CW (2008) Persistence of Helicobacter pylori in heterotrophic drinking water biofilms. Appl Environ Microbiol 74:5898–5904. https://doi.org/10.1128/AEM.00827-08 es_ES
dc.description.references Graham DY (1991) Helicobacter pylori: its epidemiology and its role in duodenal ulcer disease. J Gastroenterol Hepatol 6:105–113. https://doi.org/10.1111/j.1440-1746.1991.tb01448.x es_ES
dc.description.references Hultén K, Enroth H, Nyström T, Engstrand L (1998) Presence of Helicobacter species DNA in Swedish water. J Appli Microbiol 85:282–286. https://doi.org/10.1046/j.1365-2672.1998.00500.x es_ES
dc.description.references Iwamoto A, Tanahashi T, Okada R, Yoshida Y, Kikuchi K, Keida Y, Yoshida M (2014) Whole-genome sequencing of clarithromycin resistant Helicobacter pylori characterizes unidentified variants of multidrug resistant efflux pump genes. Gut Pathog 6:27. https://doi.org/10.1186/1757-47496-27 es_ES
dc.description.references Jiang X, Doyle MP (2002) Optimizing enrichment culture conditions for detecting Helicobacter pylori in foods. J Food Protect 65:1949–1954. https://doi.org/10.4315/0362-028x-65.12.1949 es_ES
dc.description.references Lagier JC, Edouard S, Pagnier I, Mediannikov O, Drancourt M, Raoult D (2015) Current and past strategies for bacterial culture in clinical microbiology. Clin Microbiol Rev 28(1):208–236 es_ES
dc.description.references Loke MF, Ng CG, Vilashni Y, Lim J, Ho B (2016) Understanding the dimorphic lifestyles of human gastric pathogen Helicobacter pylori using the SWATH-based proteomics approach. Sci Rep 6(26784). https://doi.org/10.1038/srep26784 es_ES
dc.description.references Lopes AI, Vale FF, Oleastro M (2014) Helicobacter pylori infection-recent developments in diagnosis. World J Gastroenterol 20:9299–9313. https://doi.org/10.3748/wjg.v20.i28.9299 es_ES
dc.description.references Lu Y, Redlonger TE, Avitia R, Galindo A, Goodman G (2002) Isolation and genotyping of Helicobacter pylori from untreated municipal wastewater. Appl Environ Microbiol 68(3):1436–1439 es_ES
dc.description.references McGee D, Geore S, Trainor E, Hortonm K, Hildebrandt E, Testerman L (2011) Cholesterol enhances Helicobacter pylori resistance to antibiotics and LL-37. Antimicrob Agents Ch 55:2897–2904. https://doi.org/10.1128/AAC.00016-11 es_ES
dc.description.references Miendje Deyi VY, Van den Borre C, Fontaine V (2010) Comparative evaluation of 3 selective media for primary isolation of Helicobacter pylori from gastric biopsies under routine conditions. Diagn Micr Infec Dis 68:474–476. https://doi.org/10.1016/j.diagmicrobio.2010.08.009 es_ES
dc.description.references Moreno Y, Ferrús MA (2012) Specific detection of cultivable Helicobacter pylori cells from wastewater treatment plant. Helicobacter 17:327-32. https://doi.org/10.1128/AEM.00827-0810.1111/j.1523-5378.2012.00961 . X es_ES
dc.description.references Moreno Y, Pérez R, Ramirez MJ, Calvet X, Santiago P, Ferrús MA (2015) Rapid identification of viable H. pylori cells in feces by DVC-FISH. JSM. Gastroenterol Hepatol 3:1049 es_ES
dc.description.references Nilsson HO, Blom J, Abu-Al-Soud W, Ljung AA, Andersen LP, Wadstrom T (2002) Effect of cold starvation, acid stress, and nutrients on metabolic activity of Helicobacter pylori. Appl Environ Microbiol 68:11–19. https://doi.org/10.1128/AEM.68.1.11-19.2002 es_ES
dc.description.references Percival S, Suleman L (2014) Biofilms and Helicobacter pylori: dissemination and persistence within the environment and host. World J Gastrointest Pathophysiol 5:122–132. https://doi.org/10.4291/wjgp.v5.i3.122 es_ES
dc.description.references Percival S, Thomas JG (2009) Transmission of Helicobacter pylori and the role of water and biofilms. J Water Health 7:469–477. https://doi.org/10.2166/wh.2009.070 es_ES
dc.description.references Ranjbar R, Khamesipour F, Jonaidi-Jafari N, Rahimi E (2016) Helicobacter pylori isolated from Iranian drinking water: vacA, cagA, iceA, oipA and babA2 genotype status and antimicrobial resistance properties. FEBS Open Bio 6:433–441. https://doi.org/10.1002/2211-5463.12054 es_ES
dc.description.references Rizvi F, Hanna A (2000) Evaluation of different transport and enrichment media for the isolation of Helicobacter pylori. JAMC 12:31–33 http://ayubmed.edu.pk/JAMC/PAST/12-3/Farhat.pdf es_ES
dc.description.references Santiago P, Moreno Y, Ferrús MA (2015) Identification of viable Helicobacter pylori in drinking water supplies by cultural and molecular techniques. Helicobacter 20:252–259. https://doi.org/10.1111/hel.12205 es_ES
dc.description.references Shibayama K, Nagasawa M, Ando T, Minami M, Wachino J, Suzuki S, Arakawa Y (2006) Usefulness of adult bovine serum for Helicobacter pylori culture media. J Clin Microbiol 44(11):4255–4257 es_ES
dc.description.references Sjomina O, Pavlova J, Niv Y, Leja M (2018) Epidemiology of Helicobacter pylori infection Helicobacter e12514. https://doi.org/10.1111/hel.12514 , Epidemiology ofHelicobacter pyloriinfection, 23 es_ES
dc.description.references Stevenson TH, Lucia LM, Acuff GR (2000) Development of a selective medium for isolation of Helicobacter pylori from cattle and beef samples. App Environ Microbiol 66:723–727 es_ES
dc.description.references Suzuki R, Shiota S, Yamoka Y (2012) Molecular epidemiology, population genetics, and pathogenic role of Helicobacter pylori. Infect Genet Evol 12:203–2013. https://doi.org/10.1016/j.meegid.2011.12.002 es_ES
dc.description.references Testerman TL, Conn PB, Mobley HLT, McGee DJ (2006) Nutritional requirements and antibiotic resistance patterns of Helicobacter species in chemically defined media. J Clin Microbiol 44:1650–1658. https://doi.org/10.1128/JCM.44.5.1650-1658.2006 es_ES
dc.description.references Van Tongeren SP, Slaets JP, Harmsen HJM, Welling GW (2005) Fecal microbiota composition and frailty. Appl Environ Microbiol 71:6438–6442. https://doi.org/10.1128/AEM.71.10.6438-6442.2005 es_ES
dc.description.references Wang YK, Kuo FC, Liu CJ, Wu MC, Shih HY, Wang SS, Wu JY, Kuo CH, Huang YK, Wu DC (2015) Diagnosis of Helicobacter pylori infection: current options and developments. World J Gastroenterol 21:11221–11235. https://doi.org/10.3748/wjg.v21.i40.11221 es_ES


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem