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dc.contributor.author | Hortelano, Irene | es_ES |
dc.contributor.author | Moreno Trigos, Mª Yolanda | es_ES |
dc.contributor.author | Vesga, Fidson Juarismi | es_ES |
dc.contributor.author | Ferrús Pérez, Mª Antonia | es_ES |
dc.date.accessioned | 2021-02-19T04:33:59Z | |
dc.date.available | 2021-02-19T04:33:59Z | |
dc.date.issued | 2020-11 | es_ES |
dc.identifier.issn | 1139-6709 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/161856 | |
dc.description.abstract | [EN] The objective of the present study was to establish the most suitable culture medium for the isolation ofH. pylorifrom environmental and clinical samples. Ten different culture media were compared and evaluated. Four of them had been previously described and were modified in this study. The rest of the media were designed de novo. Three different matrices, tap water, wastewater, and feces, were inoculated with serial dilutions ofH. pyloriNCTC 11637 strain at a final concentration of 10(4)and 10(3) CFU/ml and the recovery rates were calculated. From inoculated tap water and wastewater samples,H. pyloricolonies were recovered from four out of the analyzed culture media. When fecal samples were analyzed, the isolation of the pathogen under study was only possible from two culture media. Different optimal media were observed for each type of sample, even for wastewater and stool samples. Nevertheless, our results indicated that the combination of Dent Agar with polymyxin B sulfate did not inhibit the growth ofH. pyloriand was highly selective for its recovery, regardless of the sample origin. Thus, we propose the use of this medium as a diagnostic tool for the isolation ofH. pylorifrom environmental and clinical samples, as well as for epidemiological studies. | es_ES |
dc.description.sponsorship | This work was supported by the Conselleria de Educacion, Investigacion, Cultura y Deporte, of the Community of Valencia, Spain, under project AICO/2018/273, and by the Spanish Ministry of Economy and Competitiveness AGL2014/53875-R Grant. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Springer-Verlag | es_ES |
dc.relation.ispartof | International Microbiology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | H. pylori | es_ES |
dc.subject | Isolation | es_ES |
dc.subject | Identification | es_ES |
dc.subject | Culture media | es_ES |
dc.subject | Feces | es_ES |
dc.subject | Environmental samples | es_ES |
dc.subject.classification | MICROBIOLOGIA | es_ES |
dc.title | Evaluation of different culturemedia for detection and quantification of H. pylori in environmental and clinical samples | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1007/s10123-020-00135-z | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2014-53875-R/ES/H. PYLORI Y OTROS HELICOBACTERS PATOGENOS EN AGUAS Y ALIMENTOS: DESARROLLO Y APLICACION DE HERRAMIENTAS MOLECULARES DIRIGIDAS A LA EVALUACION DEL RIESGO PARA EL CONSUMIDOR/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//AICO%2F2018%2F273/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario de Ingeniería del Agua y del Medio Ambiente - Institut Universitari d'Enginyeria de l'Aigua i Medi Ambient | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.description.bibliographicCitation | Hortelano, I.; Moreno Trigos, MY.; Vesga, FJ.; Ferrús Pérez, MA. (2020). Evaluation of different culturemedia for detection and quantification of H. pylori in environmental and clinical samples. International Microbiology. 23(4):481-487. https://doi.org/10.1007/s10123-020-00135-z | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1007/s10123-020-00135-z | es_ES |
dc.description.upvformatpinicio | 481 | es_ES |
dc.description.upvformatpfin | 487 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 23 | es_ES |
dc.description.issue | 4 | es_ES |
dc.identifier.pmid | 32607781 | es_ES |
dc.relation.pasarela | S\415175 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Economía y Empresa | es_ES |
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