- -

The Potyviridae P1a leader protease contributes to host range specificity

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

The Potyviridae P1a leader protease contributes to host range specificity

Mostrar el registro sencillo del ítem

Ficheros en el ítem

Thumbnail
Nombre: Shan;Pasin;Valli - ...
Tamaño: 2.595Mb
Formato: PDF
Descripción: Versión del Autor.
Abrir
[Cerrado]
Nombre: 1-s2.0-S004268221 ...
Tamaño: 3.216Mb
Formato: PDF
Descripción: Versión editorial
Solicitar una copia al autor
dc.contributor.author Shan, Hongying es_ES
dc.contributor.author Pasin, Fabio es_ES
dc.contributor.author Valli, Adrian es_ES
dc.contributor.author Castillo, Carla es_ES
dc.contributor.author Rajulu, Charukesi es_ES
dc.contributor.author CARBONELL, ALBERTO es_ES
dc.contributor.author Simon-Mateo, Carmen es_ES
dc.contributor.author Garcia, Juan Antonio es_ES
dc.contributor.author Rodamilans, Bernardo es_ES
dc.date.accessioned 2021-02-25T04:49:14Z
dc.date.available 2021-02-25T04:49:14Z
dc.date.issued 2015-02 es_ES
dc.identifier.issn 0042-6822 es_ES
dc.identifier.uri http://hdl.handle.net/10251/162361
dc.description.abstract [EN] The P1a protein of the ipomovirus Cucumber vein yellowing virus is one of the self-cleavage serine proteases present in Potyviridae family members. P1a is located at the N-terminal end of the viral polyprotein, and is closely related to potyviral P1 protease. For its proteolytic activity, P1a requires a still unknown host factor; this might be linked to involvement in host specificity. Here we built a series of constructs and chimeric viruses to help elucidate the role of P1a cleavage in host range definition. We demonstrate that host-dependent separation of P1a from the remainder of the polyprotein is essential for suppressing RNA silencing defenses and for efficient viral infection. Intergenus replacement of leader proteases could broaden host range definition, as shown by the local infection capacity of viral chimeras. These findings support the role of viral proteases as important determinants in host adaptation. es_ES
dc.description.sponsorship H.S. is supported by the China Scholarship Council; F.P. and C.R. were financed by La Caixa PhD fellowships, and C.C. by a scholarship from the Spanish government (MAEC-AECID). This work was funded by Grants BIO2013-49053-R and Plant-KBBE PCIN-2013-056 from the Spanish Ministerio de Economia y Competitividad. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Virology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Potyviriadae es_ES
dc.subject P1a es_ES
dc.subject Host range es_ES
dc.subject P1 proteases es_ES
dc.subject RNA silencing suppression es_ES
dc.subject Plum pox virus es_ES
dc.subject Cucumber vein yellowing virus es_ES
dc.title The Potyviridae P1a leader protease contributes to host range specificity es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.virol.2014.12.013 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2013-49053-R/ES/DESCIFRANDO LAS INTERACCIONES PLANTA-VIRUS PARA EL DESARROLLO DE NUEVAS ESTRATEGIAS PARA CONTROLAR LA INFECCION DEL VIRUS DE LA SHARKA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//PCIN-2013-056/ES/COMBINACION DE BIOLOGIA DE SISTEMAS Y ABORDAJES EXPERIMENTALES DE ALTO RENDIMIENTO PARA APORTAR RESISTENCIA DURADERARA A VIRUS DE PLANTAS EN ESPECIES DE INTERES AGRONOMICO/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Shan, H.; Pasin, F.; Valli, A.; Castillo, C.; Rajulu, C.; Carbonell, A.; Simon-Mateo, C.... (2015). The Potyviridae P1a leader protease contributes to host range specificity. Virology. 476:264-270. https://doi.org/10.1016/j.virol.2014.12.013 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.virol.2014.12.013 es_ES
dc.description.upvformatpinicio 264 es_ES
dc.description.upvformatpfin 270 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 476 es_ES
dc.identifier.pmid 25562450 es_ES
dc.relation.pasarela S\378034 es_ES
dc.contributor.funder China Scholarship Council es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Fundació Bancària Caixa d'Estalvis i Pensions de Barcelona es_ES
dc.description.references ADAMS, M. J., ANTONIW, J. F., & BEAUDOIN, F. (2005). Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Molecular Plant Pathology, 6(4), 471-487. doi:10.1111/j.1364-3703.2005.00296.x es_ES
dc.description.references Brigneti, G. (1998). Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. The EMBO Journal, 17(22), 6739-6746. doi:10.1093/emboj/17.22.6739 es_ES
dc.description.references Calvo, M., Malinowski, T., & García, J. A. (2014). Single Amino Acid Changes in the 6K1-CI Region Can Promote the Alternative Adaptation of Prunus- and Nicotiana-Propagated Plum pox virus C Isolates to Either Host. Molecular Plant-Microbe Interactions®, 27(2), 136-149. doi:10.1094/mpmi-08-13-0242-r es_ES
dc.description.references Cambra, M., Capote, N., Myrta, A., & Llácer, G. (2006). Plum pox virus and the estimated costs associated with sharka disease. EPPO Bulletin, 36(2), 202-204. doi:10.1111/j.1365-2338.2006.01027.x es_ES
dc.description.references Carbonell, A., Dujovny, G., García, J. A., & Valli, A. (2012). The Cucumber vein yellowing virus Silencing Suppressor P1b Can Functionally Replace HCPro in Plum pox virus Infection in a Host-Specific Manner. Molecular Plant-Microbe Interactions®, 25(2), 151-164. doi:10.1094/mpmi-08-11-0216 es_ES
dc.description.references Carrington, J. C., Freed, D. D., & Sanders, T. C. (1989). Autocatalytic processing of the potyvirus helper component proteinase in Escherichia coli and in vitro. Journal of Virology, 63(10), 4459-4463. doi:10.1128/jvi.63.10.4459-4463.1989 es_ES
dc.description.references Chung, B. Y.-W., Miller, W. A., Atkins, J. F., & Firth, A. E. (2008). An overlapping essential gene in the Potyviridae. Proceedings of the National Academy of Sciences, 105(15), 5897-5902. doi:10.1073/pnas.0800468105 es_ES
dc.description.references García, J. A., Glasa, M., Cambra, M., & Candresse, T. (2014). Plum pox virusand sharka: a model potyvirus and a major disease. Molecular Plant Pathology, 15(3), 226-241. doi:10.1111/mpp.12083 es_ES
dc.description.references Garcia-Ruiz, H., Takeda, A., Chapman, E. J., Sullivan, C. M., Fahlgren, N., Brempelis, K. J., & Carrington, J. C. (2010). Arabidopsis RNA-Dependent RNA Polymerases and Dicer-Like Proteins in Antiviral Defense and Small Interfering RNA Biogenesis during Turnip Mosaic Virus Infection  . The Plant Cell, 22(2), 481-496. doi:10.1105/tpc.109.073056 es_ES
dc.description.references Gibson, D. G., Young, L., Chuang, R.-Y., Venter, J. C., Hutchison, C. A., & Smith, H. O. (2009). Enzymatic assembly of DNA molecules up to several hundred kilobases. Nature Methods, 6(5), 343-345. doi:10.1038/nmeth.1318 es_ES
dc.description.references Herrera, G. (2013). Investigations of the Plum pox virus in Chile in the past 20 years. Chilean journal of agricultural research, 73(1), 60-65. doi:10.4067/s0718-58392013000100009 es_ES
dc.description.references Ivanov, K. I., Eskelin, K., Lõhmus, A., & Mäkinen, K. (2014). Molecular and cellular mechanisms underlying potyvirus infection. Journal of General Virology, 95(7), 1415-1429. doi:10.1099/vir.0.064220-0 es_ES
dc.description.references Janssen, D., Martín, G., Velasco, L., Gómez, P., Segundo, E., Ruiz, L., & Cuadrado, I. M. (2005). Absence of a coding region for the helper component-proteinase in the genome of cucumber vein yellowing virus, a whitefly-transmitted member of the Potyviridae. Archives of Virology, 150(7), 1439-1447. doi:10.1007/s00705-005-0515-z es_ES
dc.description.references Kasschau, K. D., & Carrington, J. C. (1998). A Counterdefensive Strategy of Plant Viruses. Cell, 95(4), 461-470. doi:10.1016/s0092-8674(00)81614-1 es_ES
dc.description.references Lackner, T., Müller, A., Pankraz, A., Becher, P., Thiel, H.-J., Gorbalenya, A. E., & Tautz, N. (2004). Temporal Modulation of an Autoprotease Is Crucial for Replication and Pathogenicity of an RNA Virus. Journal of Virology, 78(19), 10765-10775. doi:10.1128/jvi.78.19.10765-10775.2004 es_ES
dc.description.references Li, W., Hilf, M. E., Webb, S. E., Baker, C. A., & Adkins, S. (2008). Presence of P1b and absence of HC-Pro in Squash vein yellowing virus suggests a general feature of the genus Ipomovirus in the family Potyviridae. Virus Research, 135(2), 213-219. doi:10.1016/j.virusres.2008.03.015 es_ES
dc.description.references Maliogka, V. I., Calvo, M., Carbonell, A., García, J. A., & Valli, A. (2012). Heterologous RNA-silencing suppressors from both plant- and animal-infecting viruses support plum pox virus infection. Journal of General Virology, 93(7), 1601-1611. doi:10.1099/vir.0.042168-0 es_ES
dc.description.references MALIOGKA, V. I., SALVADOR, B., CARBONELL, A., SÁENZ, P., LEÓN, D. S., OLIVEROS, J. C., … SIMÓN-MATEO, C. (2012). Virus variants with differences in the P1 protein coexist in a Plum pox virus population and display particular host-dependent pathogenicity features. Molecular Plant Pathology, 13(8), 877-886. doi:10.1111/j.1364-3703.2012.00796.x es_ES
dc.description.references Martinez, F., & Daros, J.-A. (2014). Tobacco etch virus Protein P1 Traffics to the Nucleolus and Associates with the Host 60S Ribosomal Subunits during Infection. Journal of Virology, 88(18), 10725-10737. doi:10.1128/jvi.00928-14 es_ES
dc.description.references Pasin, F., Kulasekaran, S., Natale, P., Simón-Mateo, C., & García, J. (2014). Rapid fluorescent reporter quantification by leaf disc analysis and its application in plant-virus studies. Plant Methods, 10(1), 22. doi:10.1186/1746-4811-10-22 es_ES
dc.description.references Pasin, F., Simón-Mateo, C., & García, J. A. (2014). The Hypervariable Amino-Terminus of P1 Protease Modulates Potyviral Replication and Host Defense Responses. PLoS Pathogens, 10(3), e1003985. doi:10.1371/journal.ppat.1003985 es_ES
dc.description.references Peng, C.-W., Peremyslov, V. V., Mushegian, A. R., Dawson, W. O., & Dolja, V. V. (2001). Functional Specialization and Evolution of Leader Proteinases in the Family Closteroviridae. Journal of Virology, 75(24), 12153-12160. doi:10.1128/jvi.75.24.12153-12160.2001 es_ES
dc.description.references Rodamilans, B., Valli, A., & García, J. A. (2013). Mechanistic divergence between P1 proteases of the family Potyviridae. Journal of General Virology, 94(6), 1407-1414. doi:10.1099/vir.0.050781-0 es_ES
dc.description.references Romay, G., Lecoq, H., & Desbiez, C. (2013). Zucchini tigré mosaic virus is a distinct potyvirus in the papaya ringspot virus cluster: molecular and biological insights. Archives of Virology, 159(2), 277-289. doi:10.1007/s00705-013-1798-0 es_ES
dc.description.references SALVADOR, B., SAÉNZ, P., YANGÜEZ, E., QUIOT, J. B., QUIOT, L., DELGADILLO, M. O., … SIMÓN-MATEO, C. (2008). Host-specific effect of P1 exchange between two potyviruses. Molecular Plant Pathology, 9(2), 147-155. doi:10.1111/j.1364-3703.2007.00450.x es_ES
dc.description.references Schägger, H., & von Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry, 166(2), 368-379. doi:10.1016/0003-2697(87)90587-2 es_ES
dc.description.references Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9(7), 671-675. doi:10.1038/nmeth.2089 es_ES
dc.description.references ŠUBR, Z., & GLASA, M. (2013). Unfolding the secrets of plum pox virus: from epidemiology to genomics. Acta virologica, 57(02), 217-228. doi:10.4149/av_2013_02_217 es_ES
dc.description.references Valli, A., López-Moya, J. J., & García, J. A. (2007). Recombination and gene duplication in the evolutionary diversification of P1 proteins in the family Potyviridae. Journal of General Virology, 88(3), 1016-1028. doi:10.1099/vir.0.82402-0 es_ES
dc.description.references Valli, A., Martín-Hernández, A. M., López-Moya, J. J., & García, J. A. (2006). RNA Silencing Suppression by a Second Copy of the P1 Serine Protease ofCucumber Vein Yellowing Ipomovirus, a Member of the FamilyPotyviridaeThat Lacks the Cysteine Protease HCPro. Journal of Virology, 80(20), 10055-10063. doi:10.1128/jvi.00985-06 es_ES
dc.description.references Verchot, J., & Carrington, J. C. (1995). Debilitation of plant potyvirus infectivity by P1 proteinase-inactivating mutations and restoration by second-site modifications. Journal of Virology, 69(3), 1582-1590. doi:10.1128/jvi.69.3.1582-1590.1995 es_ES
dc.description.references Verchot, J., & Carrington, J. C. (1995). Evidence that the potyvirus P1 proteinase functions in trans as an accessory factor for genome amplification. Journal of Virology, 69(6), 3668-3674. doi:10.1128/jvi.69.6.3668-3674.1995 es_ES
dc.description.references Verchot, J., Herndon, K. L., & Carrington, J. C. (1992). Mutational analysis of the tobacco etch potyviral 35-kDa proteinase: Identification of essential residues and requirements for autoproteolysis. Virology, 190(1), 298-306. doi:10.1016/0042-6822(92)91216-h es_ES
dc.description.references Voinnet, O., Rivas, S., Mestre, P., & Baulcombe, D. (2003). Retracted: An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. The Plant Journal, 33(5), 949-956. doi:10.1046/j.1365-313x.2003.01676.x es_ES


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem