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Optical imaging of voltage and calcium in isolated hearts: Linking spatiotemporal heterogeneities and ventricular fibrillation initiation

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Optical imaging of voltage and calcium in isolated hearts: Linking spatiotemporal heterogeneities and ventricular fibrillation initiation

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dc.contributor.author Hernández-Romero, Ismael es_ES
dc.contributor.author Guillem Sánchez, María Salud es_ES
dc.contributor.author Figuera, Carlos es_ES
dc.contributor.author Atienza, Felipe es_ES
dc.contributor.author Fernández-Avilés, Francisco es_ES
dc.contributor.author Martínez Climent, Batiste Andreu es_ES
dc.date.accessioned 2021-03-01T08:10:12Z
dc.date.available 2021-03-01T08:10:12Z
dc.date.issued 2019-05-14 es_ES
dc.identifier.issn 1932-6203 es_ES
dc.identifier.uri http://hdl.handle.net/10251/162604
dc.description.abstract [EN] Background Alternans have been associated with the development of ventricular fibrillation and its control has been proposed as antiarrhythmic strategy. However, cardiac arrhythmias are a spatiotemporal phenomenon in which multiple factors are involved (e.g. calcium and voltage spatial alternans or heterogeneous conduction velocity) and how an antiarrhythmic drug modifies these factors is poorly understood. Objective The objective of the present study is to evaluate the relation between spatial electrophysiological properties (i.e. spatial discordant alternans and conduction velocity) and the induction of ventricular fibrillation (VF) when a calcium blocker is applied. Methods The mechanisms of initiation of VF were studied by simultaneous epicardial voltage and calcium optical mapping in isolated rabbit hearts using an incremental fast pacing protocol. The additional value of analyzing spatial phenomena in the generation of unidirectional blocks and reentries as precursors of VF was depicted. Specifically, the role of action potential duration (APD), calcium transients (CaT), spatial alternans and conduction velocity in the initiation of VF was evaluated during basal conditions and after the administration of verapamil. Results Our results enhance the relation between (1) calcium spatial alternans and (2) slow conduction velocities with the dynamic creation of unidirectional blocks that allowed the induction of VF. In fact, the administration of verapamil demonstrated that calcium and not voltage spatial alternans were the main responsible for VF induction. Conclusions VF induction at high activation rates was linked with the concurrence of a low conduction velocity and high magnitude of calcium alternans, but not necessarily related with increases of APD. Verapamil can postpone the development of cardiac alternans and the apparition of ventricular arrhythmias. es_ES
dc.description.sponsorship This work was funded in part by the CIBERCV (Centro de Investigacion Biomedica en Red Enfermedades Cardiovasculares), Instituto de Salud Carlos III (PI14/00857, PI16/01123, DTS16/0160, PI17/01059, PI17/01106 and IJCI-2014-22178); Spanish Ministry of Ecomomy (TEC2013-46067-R); Generalitat Valenciana Grants (APOSTD/2017 and APOSTD/2018) and projects (GVA/2018/103), EIT-Health 19600 AFFINE and cofound by FEDER. es_ES
dc.language Inglés es_ES
dc.publisher Public Library of Science es_ES
dc.relation.ispartof PLoS ONE es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject.classification TECNOLOGIA ELECTRONICA es_ES
dc.title Optical imaging of voltage and calcium in isolated hearts: Linking spatiotemporal heterogeneities and ventricular fibrillation initiation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1371/journal.pone.0215951 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//PI14%2F00857/ES/Caracterización No-invasiva de los Mecanismos de Mantenimiento de la Fibrilación Auricular. Estudio PERSONALIZE-AF/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//APOSTD%2F2017 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//APOSTD%2F2018%2F032/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//TEC2013-46067-R/ES/ESTIMACION NO INVASIVA DE LA ACTIVIDAD ELECTRICA CARDIACA MEDIANTE OPTIMIZACION CONVEXA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//DTS16%2F00160/ES/Guiado en Tiempo Real de la Ablación de la Fibrilación Auricular mediante Cartografía Eléctrica Global (CORIFY)/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//PI16%2F01123/ES/Regeneración Cardiaca de Infarto Crónico Porcino mediante Inyecciónes Intramiocardiacas de Células Progenitoras Embebidas en Hidrogeles de Matriz Decelularizada/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//IJCI-2014-22178/ES/IJCI-2014-22178/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//PI17%2F01059/ES/Estratificación y tratamiento de la fibrilación auricular basada en los mecanismos de perpetuación de la arritmia/STRATIFY-AF/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//GV%2F2018%2F103/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ISCIII//PI17%2F01106/ES/Estratificación y tratamiento de la fibrilación auricular basada en los mecanismos de perpetuación de la arritmia/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería Electrónica - Departament d'Enginyeria Electrònica es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Telecomunicación y Aplicaciones Multimedia - Institut Universitari de Telecomunicacions i Aplicacions Multimèdia es_ES
dc.description.bibliographicCitation Hernández-Romero, I.; Guillem Sánchez, MS.; Figuera, C.; Atienza, F.; Fernández-Avilés, F.; Martínez Climent, BA. (2019). Optical imaging of voltage and calcium in isolated hearts: Linking spatiotemporal heterogeneities and ventricular fibrillation initiation. PLoS ONE. 14(5):1-15. https://doi.org/10.1371/journal.pone.0215951 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1371/journal.pone.0215951 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 15 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 14 es_ES
dc.description.issue 5 es_ES
dc.identifier.pmid 31086382 es_ES
dc.identifier.pmcid PMC6516663 es_ES
dc.relation.pasarela S\409195 es_ES
dc.contributor.funder EIT Health es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder Centro de Investigación Biomédica en Red Enfermedades Cardiovasculares es_ES
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