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A qPCR-based method for the detection and quantification of the peach powdery mildew (Podosphaera pannosa) in epidemiological studies

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A qPCR-based method for the detection and quantification of the peach powdery mildew (Podosphaera pannosa) in epidemiological studies

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Marimón, N.; Eduardo, I.; León Santana, M.; Berbegal Martinez, M.; Armengol Fortí, J.; Luque, J. (2020). A qPCR-based method for the detection and quantification of the peach powdery mildew (Podosphaera pannosa) in epidemiological studies. European Journal of Plant Pathology. 158(4):1005-1016. https://doi.org/10.1007/s10658-020-02136-0

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Title: A qPCR-based method for the detection and quantification of the peach powdery mildew (Podosphaera pannosa) in epidemiological studies
Author: Marimón, N. Eduardo, Iban León Santana, Maela Berbegal Martinez, Monica Armengol Fortí, Josep Luque, J.
UPV Unit: Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals
Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani
Issued date:
Abstract:
[EN] A qPCR-based method was developed to detect and quantifyPodosphaera pannosa, the main causal agent of peach powdery mildew. A primer pair was designed to target part of the ITS region of the fungal ribosomal DNA, which ...[+]
Subjects: Aerobiology , Epidemiology , Molecular technique , Powdery mildew , Prunus persica
Copyrigths: Reserva de todos los derechos
Source:
European Journal of Plant Pathology. (issn: 0929-1873 )
DOI: 10.1007/s10658-020-02136-0
Publisher:
Springer-Verlag
Publisher version: https://doi.org/10.1007/s10658-020-02136-0
Project ID:
INIA/RTA2013-00004-C03-01
INIA/CPD-2015-0142
Thanks:
This research was funded by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Spain, project RTA2013-00004-C03-01, and with matching funds from the European Regional Development Fund (ERDF). ...[+]
Type: Artículo

References

Amano, K. (1986). Host range and geographical distribution of the powdery mildew fungi (p. 741). Tokyo: Japan Scientific Societies Press.

Armbruster, D. A., & Pry, T. (2008). Limit of blank, limit of detection and limit of quantitation. The Clinical Biochemist Reviews, 2(suppl. 1), 49–52.

Braun, U. (1987). A monograph of the Erysiphales (powdery mildews). Nova Hedwigia, 89, 1-700. Stuttgart: J. Cramer. [+]
Amano, K. (1986). Host range and geographical distribution of the powdery mildew fungi (p. 741). Tokyo: Japan Scientific Societies Press.

Armbruster, D. A., & Pry, T. (2008). Limit of blank, limit of detection and limit of quantitation. The Clinical Biochemist Reviews, 2(suppl. 1), 49–52.

Braun, U. (1987). A monograph of the Erysiphales (powdery mildews). Nova Hedwigia, 89, 1-700. Stuttgart: J. Cramer.

Braun, U., Cook, R. T. A., Inman, A. J., & Shin, H. D. (2002). The taxonomy of powdery mildew fungi. In R. R. Bélanger, W. R. Bushnell, A. J. Dik, & T. L. W. Carver (Eds.), The Powdery Mildews, A Comprenhensive Treatise (pp. 13–55). St. Paul: APS Press.

Butt, D. J. (1978). Epidemiology of powdery mildews. In D. M. Spencer (Ed.), The Powdery Mildews (pp. 51–81). New York: Academic.

Cao, X., Yao, D., Xu, X., Zhou, Y., Ding, K., Duan, X., Fan, J., & Luo, Y. (2015). Development of weather- and airborne inoculum-based models to describe disease severity of wheat powdery mildew. Plant Disease, 99, 395–400.

Cunnington, J. H., Lawrie, A. C., & Pascoe, I. G. (2005). Genetic variation within Podosphaera tridactyla reveals a paraphyletic species complex with biological specialization towards specific Prunus subgenera. Mycological Research, 119, 357–362.

Donoso, J. M., Picañol, R., Serra, O., Howad, W., Alegre, S., Arús, P., & Eduardo, I. (2016). Exploring almond genetic variability useful for peach improvement: mapping major genes and QTLs in two interspecific almond x peach populations. Molecular Breeding, 36, 1–17.

Dung, J. K. S., Scott, J. C., & Cheng, Q. (2018). Detection and quantification of airborne Claviceps purpurea sensu lato ascospores from Hirst-type spore traps using Real-Time Quantitative PCR. Plant Disease, 102, 2487–2493.

Falacy, J. S., Grove, G. G., Mahaffee, W. F., Galloway, H., Glawe, D. A., Larsen, R. C., & Vandemark, G. J. (2007). Detection of Erysiphe necator in air samples using the polymerase chain reaction and species-specific primers. Phytopathology, 97, 1290–1297.

Farr, D. F., & Rossman, A. Y. (2019). Fungal Databases, U.S. National Fungus Collections, ARS, USDA. Retrieved February 25, 2019, from https://nt.ars-grin.gov/fungaldatabases/.

Galán, C., Cariñanos, P., Alcázar, P., & Dominguez, E. (2007). Management and Quality Manual. Spanish Aerobiology Network (REA). Córdoba: Servicio de Publicaciones de la Universidad de Córdoba.

Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology, 2, 113–118.

Grove, G. G. (1991). Powdery mildew of sweet cherry: Influence of temperature and wetness duration on release and germination of ascospores of Podosphaera clandestina. Phytopathology, 81, 1271–1275.

Grove, G. G. (1995). Powdery mildew. In J. M. Ogawa, E. I. Zehr, G. W. Bird, D. F. Ritchie, K. Uriu, & J. K. Uyemoto (Eds.), Compendium of Stone Fruit Diseases (pp. 12–14). St. Paul: APS Press.

Hollomon, D. W., & Wheeler, I. E. (2002). Controlling powdery mildews with chemistry. In R. R. Bélanger, W. R. Bushnell, A. J. Dik, & T. L. W. Carver (Eds.), The Powdery Mildews, A Comprehensive Treatise (pp. 249–255). Saint Paul: APS Press.

Horst, R. K., & Cloyd, R. A. (2007). Powdery mildews. In R. K. Horst & R. A. Cloyd (Eds.), Compendium of Rose Diseases and Pests (pp. 5–8). Saint Paul: APS Press.

Ito, M., & Takamatsu, S. (2010). Molecular phylogeny and evolution of subsection Magnicellulatae (Erysiphaceae: Podosphaera) with special reference to host plants. Mycoscience, 51, 34–43.

Jarvis, W. R., Gubler, W. D., & Grove, G. G. (2002). Epidemiology of powdery mildews in agricultural pathosystems. In R. R. Bélanger, W. R. Bushnell, A. J. Dik, & T. L. W. Carver (Eds.), The Powdery Mildews, A Comprehensive Treatise (pp. 169–199). Saint Paul: APS Press.

Kunjeti, S. G., Anchieta, A., Martin, F. N., Choi, Y.-J., Thines, M., Michelmore, R. W., … Klosterman, S. J. (2016). Detection and quantification of Bremia lactucae by spore trapping and quantitative PCR. Phytopathology, 106, 1426–1437.

Leus, L., Dewitte, A., Van Huylenbroeck, J., Vanhoutte, N., Van Bockstaele, E., & Hofte, M. (2006). Podosphaera pannosa (syn. Sphaerotheca pannosa) on Rosa and Prunus spp.: characterization of pathotypes by differential plant reactions and ITS sequences. Journal of Phytopathology, 154, 23–28.

Longrée, K. (1939). The Effect of Temperature and Relative Humidity on Powdery Mildew of Roses. New York: Agricultural Experiment Station Ithaca.

Luque, J., Martos, S., & Phillips, A. J. L. (2005). Botryosphaeria viticola sp. nov. on grapevines: a new species with a Dothiorella anamorph. Mycologia, 97, 1111–1121.

Mahaffee, W. F., & Stoll, R. (2016). The ebb and flow of airborne pathogens: Monitoring and use in disease management decisions. Phytopathology, 106, 420–431.

MAPA (2002). Real Decreto 1201/2002, de 20 de noviembre, por el que se regula la producción integrada de productos agrícolas. https://www.boe.es/boe/dias/2002/11/30/pdfs/A42028-42040.pdf. Accessed 11 Dec 2020.

Marimon, N., Eduardo, I., Martínez-Minaya, J., Vicent, A., & Luque, J. (2020). A decision support system based on degree-days to initiate fungicide spray programs for peach powdery mildew in Catalonia, Spain. Plant Disease. https://doi.org/10.1094/PDIS-10-19-2130-RE.

Michailides, T. J., Morgan, D. P., Ma, Z., Luo, Y., Felts, D., Doster, M. A., & Reyes, H. (2005). Conventional and molecular assays aid diagnosis of crop diseases and fungicide resistance. California Agriculture, 59, 115–123.

Ogawa, J. M., & English, H. (1991). Diseases of temperate zone tree fruit and nut crops (3345, pp. 461). Oakland: University of California, Division of Agriculture and Natural Resources.

R Core Team (2019). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna . https://www.R-project.org/.

Reuveni, M. (2001). Improved control of powdery mildew (Sphaerotheca pannosa) of nectarines in Israel using strobilurin and polyoxin B fungicides; mixtures with sulfur; and early bloom applications. Crop Protection, 20, 663–668.

Sholberg, P., O’Gorman, D., Bedford, K., & Lévesque, C. A. (2005). Development of a DNA microarray for detection and monitoring of economically important apple diseases. Plant Disease, 89, 1143–1150.

Takamatsu, S., Niinomi, S., Harada, M., & Havrylenko, M. (2010). Molecular phylogenetic analyses reveal a close evolutionary relationship between Podosphaera (Erysiphales: Erysiphaceae) and its rosaceous hosts. Persoonia, 24, 38–48.

Thiessen, L. D., Keune, J. A., Neill, T. M., Turecheck, W. W., Grove, G. G., & Mahaffee, W. F. (2016). Development of a grower-conducted inoculum detection assay for management of grape powdery mildew. Plant Pathology, 65, 238–249.

Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.

Toma, S., Ivascu, A., & Oprea, M. (1998). Highlights of epidemiology of the fungus Sphaerotheca pannosa (Wallr.) Lev. var. persicae Woron in the southern zone of Romania. Acta Horticulturae, 465, 709–714.

Weinhold, A. R. (1961). The orchard development of peach powdery mildew. Phytopathology, 51, 478–481.

White, T. J., Bruns, T. D., Lee, S. B., & Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR Protocols: A Guide to Methods and Applications (pp. 315–322). Burlington: Academic.

Yarwood, C. E. (1957). Powdery mildews. The Botanical Review, 23, 235–301.

Zúñiga, E., León, M., Berbegal, M., Armengol, J., & Luque, J. (2018). A q-PCR-based method for detection and quantification of Polystigma amygdalinum, the cause of red leaf blotch of almond. Phytopathologia Mediterranea, 57, 257–268.

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