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Metabarcoding and development of new Real-time specific assays reveal Phytophthora species diversity in Holm Oak forests in eastern Spain

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Metabarcoding and development of new Real-time specific assays reveal Phytophthora species diversity in Holm Oak forests in eastern Spain

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Nombre: Català;Berbegal;Pérez ...
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dc.contributor.author Català, Santiago es_ES
dc.contributor.author Berbegal Martinez, Monica es_ES
dc.contributor.author Pérez Sierra, Ana María es_ES
dc.contributor.author Abad Campos, Paloma es_ES
dc.date.accessioned 2021-04-17T03:33:47Z
dc.date.available 2021-04-17T03:33:47Z
dc.date.issued 2017-01 es_ES
dc.identifier.issn 0032-0862 es_ES
dc.identifier.uri http://hdl.handle.net/10251/165308
dc.description.abstract [EN] The evergreen holm oaks (Quercus ilex subsp. ilex and Q. ilex subsp. ballota) are the most representative tree species in the Iberian peninsula and the main tree species in oak-rangeland ecosystems (dehesas). Oak decline in western, central and southern parts of Spain has been associated with root rot caused by Phytophthora cinnamomi for decades. However, Phytophthora species such as P. quercina and P. psychrophila have recently been found associated with Quercus decline in eastern Spain where calcareous soils are predominant. Soil and root samples from two Quercus forests presenting decline symptoms in two different geographical areas in eastern Spain (Carrascar de la Font Roja and Vallivana) were analysed by amplicon pyrosequencing. Metabarcoding analysis showed Phytophthora species diversity, and revealed that an uncultured Phytophthora taxon, named provisionally Phytophthora taxon ballota, was the predominant species in both areas. In addition, a real-time PCR assay, based on the pyrosequencing results, was developed for the detection of this uncultured Phytophthora taxon, and also for the detection of P. quercina. TaqMan assays were tested on soil and root samples, and on Phytophthora pure cultures. The new assays showed high specificity and were consistent with metabarcoding results. A new real-time PCR protocol is proposed to evaluate the implication of different Phytophthora spp. in oak decline in eastern Spain. es_ES
dc.description.sponsorship This research was supported by the Spanish Ministerio de Ciencia e Innovacion (project AGL2011-30438-C02-01). The authors thank the Direccion General del Medio Natural from Generalitat de la Comunitat Valenciana for their support during the surveys. They are indebted to Dr Josep Armengol (Universitat Politecnica de Valencia) for critical reading of the manuscript prior to submission. es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation.ispartof Plant Pathology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Biodiversity es_ES
dc.subject ITS1 rDNA es_ES
dc.subject MMediterranean es_ES
dc.subject Metabarcoding es_ES
dc.subject Phytophthora es_ES
dc.subject Quercus es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.title Metabarcoding and development of new Real-time specific assays reveal Phytophthora species diversity in Holm Oak forests in eastern Spain es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/ppa.12541 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2011-30438-C02-01/ES/APLICACION DE TECNICAS MOLECULARES PARA VALORAR LA IMPLICACION DE PHYTOPHTHORA SPP. EN EL DECAIMIENTO DE QUERCUS ILEX/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Agroforestal Mediterráneo - Institut Agroforestal Mediterrani es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.description.bibliographicCitation Català, S.; Berbegal Martinez, M.; Pérez Sierra, AM.; Abad Campos, P. (2017). Metabarcoding and development of new Real-time specific assays reveal Phytophthora species diversity in Holm Oak forests in eastern Spain. Plant Pathology. 66(1):115-123. https://doi.org/10.1111/ppa.12541 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/ppa.12541 es_ES
dc.description.upvformatpinicio 115 es_ES
dc.description.upvformatpfin 123 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 66 es_ES
dc.description.issue 1 es_ES
dc.relation.pasarela S\332204 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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