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Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity

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Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity

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dc.contributor.author Leastro, Mikhail Oliveira es_ES
dc.contributor.author Ortega Castro, Deibis Yorlenis es_ES
dc.contributor.author Freitas-Astúa, Juliana es_ES
dc.contributor.author Kitajima, Elliot Watanabe es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author SANCHEZ NAVARRO, JESUS ANGEL es_ES
dc.date.accessioned 2021-05-07T03:32:20Z
dc.date.available 2021-05-07T03:32:20Z
dc.date.issued 2020-06-09 es_ES
dc.identifier.issn 1664-302X es_ES
dc.identifier.uri http://hdl.handle.net/10251/166059
dc.description.abstract [EN] Citrus leprosis virus C (CiLV-C) belongs to the genusCilevirus, familyKitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only thetrans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the familyKitaviridae. es_ES
dc.description.sponsorship This work was supported by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), proc. 2014/0845-9, 2017/50222-0, 2015/10249-1, and 2017/19898-8. This work was also supported by Instituto para la Formacion y Aprovechamiento de Recursos Humanos, Becas IFARHU-SENACYT, contrato 270-2018-361, grant BIO2017-88321-R from the Spanish Agencia Estatal de Investigacion (AEI), Fondo Europeo de Desarrollo Regional (FEDER), and the Prometeo Program GV2015/010 from the Generalitat Valenciana. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Microbiology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject RNA silencing suppressor es_ES
dc.subject Citrus leprosis virus C es_ES
dc.subject RSS activity es_ES
dc.subject Hypersensitive response es_ES
dc.subject FamilyKitaviridae es_ES
dc.title Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fmicb.2020.01231 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEO%2F2015%2F010/ES/Interacciones RNA-proteína y proteína-proteína en procesos de desarrollo y patogénesis mediados por virus y agentes subvirales en cultivos de interés Agronómico (RNAPROT)/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FAPESP//2017%2F50222-0/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FAPESP//2017%2F19898-8/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FAPESP//2015%2F10249-1/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FAPESP//2014%2F08458-9/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-88321-R/ES/DESCRIFRANDO INTERACCIONES VIRUS-PLANTA ESENCIALES PARA LA SUSCEPTIBILIDAD Y%2FO RESISTENCIA EN DOS PATOSISTEMAS AGRONOMICAMENTE RELEVANTES/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/SENACYT//270-2018-361/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Leastro, MO.; Ortega Castro, DY.; Freitas-Astúa, J.; Kitajima, EW.; Pallás Benet, V.; Sanchez Navarro, JA. (2020). Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity. Frontiers in Microbiology. 11:1-16. https://doi.org/10.3389/fmicb.2020.01231 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fmicb.2020.01231 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 16 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 11 es_ES
dc.identifier.pmid 32655520 es_ES
dc.identifier.pmcid PMC7325951 es_ES
dc.relation.pasarela S\433228 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Fundação de Amparo à Pesquisa do Estado de São Paulo es_ES
dc.contributor.funder Secretaría Nacional de Ciencia, Tecnología e Innovación, Panamá es_ES
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