- -

Effect of the primary cooling rate on the motility and fertility of frozen-thawed rabbit spermatozoa

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Effect of the primary cooling rate on the motility and fertility of frozen-thawed rabbit spermatozoa

Show simple item record

Files in this item

dc.contributor.author Maeda, Tatsuhiro es_ES
dc.contributor.author Liu, Enqi es_ES
dc.contributor.author Nishijima, Kazutoshi es_ES
dc.contributor.author Tanaka, M. es_ES
dc.contributor.author Yamaguchi, Shinji es_ES
dc.contributor.author Morimoto, Masatoshi es_ES
dc.contributor.author Watanabe, Teruo es_ES
dc.contributor.author Fan, Jianglin es_ES
dc.contributor.author Kitajima, Shuji
dc.date.accessioned 2012-07-19T07:39:38Z
dc.date.issued 2012-06-30
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/16715
dc.description.abstract [EN] In the present study, we examined the effect of primary cooling rates on the motility and fertility of frozen-thawed rabbit spermatozoa. Rabbit semen diluted with an egg-yolk acetamide extender was cooled from room temperature to 5°C at four different rates (-0.1, -0.2, -0.4, -0.8°C/min) as a primary cooling step, then semen was frozen in liquid nitrogen vapour. After thawing, sperm cooled at -0.1°C/min showed the highest motility (40.7 ± 7.3%); there were no significant differences between the motilities of the -0.1, -0.2, and -0.4°C/min groups. The motility of frozen-thawed sperm cooled at -0.8°C/min (29.2 ± 6.8%) was significantly lower than that of sperm cooled at -0.1 and -0.2°C/min. The viability (-0.1°C/min, 38.1 ± 4.0%; -0.8°C/min, 24.3 ± 7.3%) of frozen-thawed sperm was closely related to its motility (-0.1°C/min, 36.7 ± 7.2%; -0.8°C/min, 22.3 ± 4.7%). Quality of post-thaw motile sperm cooled at different rates was estimated by comparing the fertilisation ability of the -0.1 and -0.8°C/min groups following artificial insemination. There were no significant differences in pregnancy rates and mean litter sizes. These data suggest that cooling rabbit semen at rates ranging from -0.1 to -0.8°C/min affects the viability but not the fertilisation capacity of motile spermatozoa after thawing. es_ES
dc.description.sponsorship The authors thank Ms. T. Shimazaki, Ms. R. Tsuneyoshi, and Ms. R. Eriguchi for their technical assistance. This study was partly supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (No. 22500386).
dc.language Inglés es_ES
dc.publisher Editorial Universitat Politècnica de València es_ES
dc.relation JSPS/22500386
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject Cryopreservation es_ES
dc.subject Cooling rate es_ES
dc.subject Motility es_ES
dc.subject Spermatozoa es_ES
dc.title Effect of the primary cooling rate on the motility and fertility of frozen-thawed rabbit spermatozoa es_ES
dc.type Artículo es_ES
dc.date.updated 2012-07-19T06:40:56Z
dc.identifier.doi 10.4995/wrs.2012.1080
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Maeda, T.; Liu, E.; Nishijima, K.; Tanaka, M.; Yamaguchi, S.; Morimoto, M.; Watanabe, T.... (2012). Effect of the primary cooling rate on the motility and fertility of frozen-thawed rabbit spermatozoa. World Rabbit Science. 20(2):65-70. doi:10.4995/wrs.2012.1080 es_ES
dc.description.accrualMethod SWORD es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2012.1080
dc.description.upvformatpinicio 65
dc.description.upvformatpfin 70
dc.description.volume 20
dc.description.issue 2
dc.identifier.eissn 1989-8886
dc.contributor.funder Japan Society for the Promotion of Science


This item appears in the following Collection(s)

Show simple item record