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A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant

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A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant

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dc.contributor.author García-Fortea, Edgar es_ES
dc.contributor.author Lluch-Ruiz, Agustín es_ES
dc.contributor.author Pineda Chaza, Benito José es_ES
dc.contributor.author García-Pérez, Ana es_ES
dc.contributor.author Bracho-Gil, Juan Pablo es_ES
dc.contributor.author Plazas Ávila, María de la O es_ES
dc.contributor.author Gramazio, Pietro es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author Moreno Ferrero, Vicente es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.date.accessioned 2021-06-03T03:32:36Z
dc.date.available 2021-06-03T03:32:36Z
dc.date.issued 2020-01-06 es_ES
dc.identifier.issn 1471-2229 es_ES
dc.identifier.uri http://hdl.handle.net/10251/167212
dc.description.abstract [EN] Background Efficient organogenesis induction in eggplant (Solanum melongena L.) is required for multiple in vitro culture applications. In this work, we aimed at developing a universal protocol for efficient in vitro regeneration of eggplant mainly based on the use of zeatin riboside (ZR). We evaluated the effect of seven combinations of ZR with indoleacetic acid (IAA) for organogenic regeneration in five genetically diverse S. melongena and one S. insanum L. accessions using two photoperiod conditions. In addition, the effect of six different concentrations of indolebutyric acid (IBA) in order to promote rooting was assessed to facilitate subsequent acclimatization of plants. The ploidy level of regenerated plants was studied. Results In a first experiment with accessions MEL1 and MEL3, significant (p < 0.05) differences were observed for the four factors evaluated for organogenesis from cotyledon, hypocotyl and leaf explants, with the best results obtained (9 and 11 shoots for MEL1 and MEL3, respectively) using cotyledon tissue, 16 h light / 8 h dark photoperiod conditions, and medium E6 (2 mg/L of ZR and 0 mg/L of IAA). The best combination of conditions was tested in the other four accessions and confirmed its high regeneration efficiency per explant when using both cotyledon and hypocotyl tissues. The best rooting media was R2 (1 mg/L IBA). The analysis of ploidy level revealed that between 25 and 50% of the regenerated plantlets were tetraploid. Conclusions An efficient protocol for organogenesis of both cultivated and wild accessions of eggplant, based on the use of ZR, is proposed. The universal protocol developed may be useful for fostering in vitro culture applications in eggplant requiring regeneration of plants and, in addition, allows developing tetraploid plants without the need of antimitotic chemicals. es_ES
dc.description.sponsorship This research was funded by the Spanish Ministerio de Ciencia, Innovacion y Universidades, Agencia Estatal de Investigacion and Fondo Europeo de Desarrollo Regional (grant RTI-2018-094592-B-100 from MCIU/AEI/FEDER, UE) and by Universitat Politecnica de Valencia. The Spanish Ministerio de Educacion, Cultura y Deporte funded a predoctoral fellowship granted to Edgar Garcia-Fortea (FPU17/02389). The Generalitat Valenciana and Fondo Social Europeo funded a post-doctoral fellowship granted to Mariola Plazas (APOSTD/2018/014). The Japan Society for the Promotion of Science funded a post-doctoral fellowship granted to Pietro Gramazio (FY 2019 Postdoctoral Fellowship for Research in Japan [Standard]). The funding bodies were not involved in the design of the study, collection, analysis, interpretation of data, or drafting of the manuscript. es_ES
dc.language Inglés es_ES
dc.publisher Springer (Biomed Central Ltd.) es_ES
dc.relation JSPS/FY2019 es_ES
dc.relation AEI/RTI-2018-094592-B-100 es_ES
dc.relation GV/APOSTD/2018/014 es_ES
dc.relation MECD/FPU17/02389 es_ES
dc.relation.ispartof BMC Plant Biology es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Zeatin riboside es_ES
dc.subject Regeneration es_ES
dc.subject Somatic organogenesis es_ES
dc.subject Solanum melongena es_ES
dc.subject Tetraploids es_ES
dc.subject.classification GENETICA es_ES
dc.title A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12870-019-2215-y es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation García-Fortea, E.; Lluch-Ruiz, A.; Pineda Chaza, BJ.; García-Pérez, A.; Bracho-Gil, JP.; Plazas Ávila, MDLO.; Gramazio, P.... (2020). A highly efficient organogenesis protocol based on zeatin riboside for in vitro regeneration of eggplant. BMC Plant Biology. 20(1):1-16. https://doi.org/10.1186/s12870-019-2215-y es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1186/s12870-019-2215-y es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 16 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 20 es_ES
dc.description.issue 1 es_ES
dc.identifier.pmid 31906864 es_ES
dc.identifier.pmcid PMC6945591 es_ES
dc.relation.pasarela S\400289 es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder Japan Society for the Promotion of Science es_ES
dc.contributor.funder Ministerio de Educación, Cultura y Deporte es_ES


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