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Fostering Conservation via an Integrated Use of Conventional Approaches and High-Throughput SPET Genotyping: A Case Study Using the Endangered Canarian EndemicsSolanum lidiiandS. vespertilio (Solanaceae)

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Fostering Conservation via an Integrated Use of Conventional Approaches and High-Throughput SPET Genotyping: A Case Study Using the Endangered Canarian EndemicsSolanum lidiiandS. vespertilio (Solanaceae)

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dc.contributor.author Gramazio, Pietro es_ES
dc.contributor.author Jaén-Molina, Ruth es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.contributor.author Marrero, Águedo es_ES
dc.contributor.author Caujapé-Castells, Juli es_ES
dc.contributor.author Anderson, Gregory J. es_ES
dc.date.accessioned 2021-06-15T03:31:38Z
dc.date.available 2021-06-15T03:31:38Z
dc.date.issued 2020-07-10 es_ES
dc.identifier.uri http://hdl.handle.net/10251/167987
dc.description.abstract [EN] Islands provide unique opportunities to integrated research approaches to study evolution and conservation because boundaries are circumscribed, geological ages are often precise, and many taxa are greatly imperiled. We combined morphological and hybridization studies with high-throughput genotyping platforms to streamline relationships in the endangered monophyletic and highly diverse lineage ofSolanumin the Canarian archipelago, where three endemic taxa are currently recognized. Inter-taxa hybridizations were performed, and morphological expression was assessed with a common-garden approach. Using the eggplant Single Primer Enrichment Technology (SPET) platform with 5,093 probes, 74 individuals of three endemic taxa (Solanum lidii,S. vespertiliosubsp.vespertilio, andS. vespertiliosubsp.doramae) were sampled for SNPs. While morphological and breeding studies showed clear distinctions and some continuous variation, inter-taxon hybrids were fertile and heterotic for vigor traits. SPET genotyping revealed 1,421 high-quality SNPs and supported four, not three, distinct taxonomic entities associated with post-emergence geological, ecological and geographic factors of the islands. Given the lack of barriers to hybridization among all the taxa and their molecular differences, great care must be taken in population management. Conservation strategies must take account of the sexual and breeding systems and genotypic distribution among populations to successfully conserve and restore threatened/endangered island taxa, as exemplified bySolanumon the Canary Islands. es_ES
dc.description.sponsorship This work was supported by funds from project DEMIURGO to the Jardin Botanico Canario "Viera y Clavijo" -Unidad Asociada al CSIC of the Cabildo de Gran Canaria. Consumables and genotyping were funded by the Jardin Botanico Canario "Viera y Clavijo" -Unidad Asociada al CSIC. Participation of authors from Universitat Politecnica de Valencia has been partially funded by the European Union's Horizon 2020 Research and Innovation Programme under the Grant Agreement No. 677379 (G2P-SOL project: Linking genetic resources, genomes, and phenotypes of solanaceous crops). PG was grateful to Universitat Politecnica de Valencia and to Japan Society for the Promotion of Science for their respective Postdoctoral Grants [PAID-10-18 and FY2019-P19105 JSPS Postdoctoral Fellowship for Research in Japan (Standard)]. es_ES
dc.language Inglés es_ES
dc.publisher Frontiers Media SA es_ES
dc.relation.ispartof Frontiers in Plant Science es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Conservation es_ES
dc.subject Endangered endemics es_ES
dc.subject Reproductive biology es_ES
dc.subject SNPs es_ES
dc.subject Solanaceae es_ES
dc.subject Solanum es_ES
dc.subject Canary Islands es_ES
dc.subject SPET es_ES
dc.subject.classification GENETICA es_ES
dc.title Fostering Conservation via an Integrated Use of Conventional Approaches and High-Throughput SPET Genotyping: A Case Study Using the Endangered Canarian EndemicsSolanum lidiiandS. vespertilio (Solanaceae) es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3389/fpls.2020.00757 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/677379/EU/Linking genetic resources, genomes and phenotypes of Solanaceous crops/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/JSPS//FY2019/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UPV//PAID-10-18/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/JSPS//P19105/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Gramazio, P.; Jaén-Molina, R.; Vilanova Navarro, S.; Prohens Tomás, J.; Marrero, Á.; Caujapé-Castells, J.; Anderson, GJ. (2020). Fostering Conservation via an Integrated Use of Conventional Approaches and High-Throughput SPET Genotyping: A Case Study Using the Endangered Canarian EndemicsSolanum lidiiandS. vespertilio (Solanaceae). Frontiers in Plant Science. 11:1-17. https://doi.org/10.3389/fpls.2020.00757 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3389/fpls.2020.00757 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 11 es_ES
dc.identifier.eissn 1664-462X es_ES
dc.identifier.pmid 32754166 es_ES
dc.identifier.pmcid PMC7381301 es_ES
dc.relation.pasarela S\431035 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder Japan Society for the Promotion of Science es_ES
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dc.subject.ods 15.- Proteger, restaurar y promover la utilización sostenible de los ecosistemas terrestres, gestionar de manera sostenible los bosques, combatir la desertificación y detener y revertir la degradación de la tierra, y frenar la pérdida de diversidad biológica es_ES


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