Mostrar el registro sencillo del ítem
dc.contributor.author | Ahmad, Ejaz | es_ES |
dc.contributor.author | Naseer, Zahid | es_ES |
dc.contributor.author | Aksoy, Melih | es_ES |
dc.date.accessioned | 2021-07-06T07:05:02Z | |
dc.date.available | 2021-07-06T07:05:02Z | |
dc.date.issued | 2021-06-30 | |
dc.identifier.issn | 1257-5011 | |
dc.identifier.uri | http://hdl.handle.net/10251/168816 | |
dc.description.abstract | [EN] In the present study, we evaluated the sustaining effect of various glutathione (GSH) concentrations in extender on rabbit sperm attributes during storage at 5°C for 24 h. Semen was collected from regular donor rabbit bucks using an artificial vagina and initially evaluated for sperm quality. The qualifying ejaculates were diluted with one of the extenders having 0, 1, 2, 4 or 8 mM GSH, to achieve a final concentration of 1×108 sperm/mL. The extended samples were stored at 5°C for 24 h. Sperm motility, motion kinetics, acrosome integrity and viability were assessed after 3, 6, 12 and 24 h of storage. The results showed that total sperm motility and sperm motion kinetics (oscillation index of the sperm, straightness index and beat cross frequency) were influenced (P<0.05) by glutathione dose and refrigeration time. An interaction of (P<0.05) GSH concentrations and refrigeration time was observed for sperm viability and acrosome reaction rate. In conclusion, the 4 mM GSH supplemented extender’s protective influence was remarkable to maintainrabbit sperm quality for 24 h 5°C. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Universitat Politècnica de València | es_ES |
dc.relation.ispartof | World Rabbit Science | es_ES |
dc.rights | Reconocimiento - No comercial - Compartir igual (by-nc-sa) | es_ES |
dc.subject | Glutathione | es_ES |
dc.subject | Refrigeration | es_ES |
dc.subject | Sperm quality | es_ES |
dc.subject | Rabbit | es_ES |
dc.title | Glutathione supplementation in semen extender improves rabbit sperm attributes during refrigeration | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.4995/wrs.2021.14759 | |
dc.rights.accessRights | Abierto | es_ES |
dc.description.bibliographicCitation | Ahmad, E.; Naseer, Z.; Aksoy, M. (2021). Glutathione supplementation in semen extender improves rabbit sperm attributes during refrigeration. World Rabbit Science. 29(2):81-86. https://doi.org/10.4995/wrs.2021.14759 | es_ES |
dc.description.accrualMethod | OJS | es_ES |
dc.relation.publisherversion | https://doi.org/10.4995/wrs.2021.14759 | es_ES |
dc.description.upvformatpinicio | 81 | es_ES |
dc.description.upvformatpfin | 86 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 29 | es_ES |
dc.description.issue | 2 | es_ES |
dc.identifier.eissn | 1989-8886 | |
dc.relation.pasarela | OJS\14759 | es_ES |
dc.description.references | Aksoy M., Lehimcioğlu N.C., Akman O. 2008. Effect of seminal plasma on functional integrity of rabbit sperm membranes during storage at 4°C or freezing. World Rabbit Sci., 16: 1-6. | es_ES |
dc.description.references | https://doi.org/10.4995/wrs.2008.642 | es_ES |
dc.description.references | Ansari M.S., Rakha B.A., Ullah N., Andrabi S.M.H., Akhter S. 2011. Glutathione addition in tris-citric egg yolk extender improves the quality of cooled buffalo (Bubalus bubalis) bull semen. Pak. J. Zool., 43: 46-55. | es_ES |
dc.description.references | Bansal A.K., Bilaspuri G.S. 2011. Impacts of oxidative stress and antioxidants on semen functions. Vet. Med. Intl., Article ID 686137. https://doi.org/10.4061/2011/686137 | es_ES |
dc.description.references | Câmara D.R., Mello-Pinto M.M.C., Pinto I.C., Brasil O.O., Nunes J.F., Guerra M.M.P. 2011. Effects of reduced glutathione and catalase on the kinematics and membrane functionality of sperm during liquid storage of ram semen. Small Rumin. Res., 100: 44-49. https://doi.org/10.1016/j.smallrumres.2011.05.010 | es_ES |
dc.description.references | Darin-Bennett A., White I.G. 1977. Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology, 14: 466-470. https://doi.org/10.1016/0011-2240(77)90008-6 | es_ES |
dc.description.references | de Lamirande E., Gagnon C. 1995. Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Human Reprod., 10: 15-21. | es_ES |
dc.description.references | https://doi.org/10.1093/humrep/10.suppl_1.15 | es_ES |
dc.description.references | Domingo P., Olaciregui M., González N., De Blas I., Gil L. 2018. Effects of seminal plasma and different cryoprotectants on rabbit sperm preservation at 16°C. Exp. Anim., 67: 413-420. https://doi.org/10.1538/expanim.17-0152 | es_ES |
dc.description.references | Fadl, A., Ghallab, A., Abou-Ahmed, M. 2019. Quality assessment of cryopreserved New Zealand white rabbit spermatozoa in INRA-82 extender containing different cryoprotectants. World Rabbit Sci., 27: 77-83. https://doi.org/10.4995/wrs.2019.10892 | es_ES |
dc.description.references | Fadl A.M., Ghallab A.M., Abou-Ahmed M.M. 2020. Comparison between tris-buffer and INRA-82 extenders on the quality of chilled rabbit spermatozoa. World Rabbit Sci., 28: 13-18. https://doi.org/10.4995/wrs.2020.12287 | es_ES |
dc.description.references | Johinke D., de Graaf S.P., Bathgate R. 2014. Investigation of in vitro parameters and in vivo fertility of rabbit spermatozoa after chilled storage. Anim. Reprod. Sci., 147: 135-143. https://doi.org/10.1016/j.anireprosci.2014.04.014 | es_ES |
dc.description.references | Johinke D., de Graaf S.P., Bathgate R. 2015. The effect of sperm concentration and storage vessel on quercetin-supplemented rabbit semen during chilled storage. Reprod. Dom. Anim. 50: 567-573. https://doi.org/10.1111/rda.12525 | es_ES |
dc.description.references | Luberda Z. 2005. The role of glutathione in mammalian gametes. Reprod. Biol., 5: 5-17. | es_ES |
dc.description.references | Maya-Soriano M.J., Taberner E., Sabés-Alsina M., Piles M., Lopez-Bejar M. 2015. Absence of beneficial effects on rabbit sperm cell cryopreservation by several antioxidant agents. Zygote, 23: 1-10. https://doi.org/10.1017/S0967199413000270 | es_ES |
dc.description.references | Munsi M.N., Bhuiyan M.M.U., Majumder S., Alam M.G.S. 2007. Effects of exogenous glutathione on the quality of chilled bull semen. Reprod. Dom. Anim., 42: 358-362. https://doi.org/10.1111/j.1439-0531.2006.00789.x | es_ES |
dc.description.references | Nagy S., Sinkovics G., Kovács A. 2002. Viability and acrosome integrity of rabbit spermatozoa processed in a gelatinsupplemented extender. Anim. Reprod. Sci., 70: 283-286. | es_ES |
dc.description.references | https://doi.org/10.1016/S0378-4320(01)00189-0 | es_ES |
dc.description.references | Naseer Z., Ahmad E., Aksoy M., Epikmen E. 2020. Impact of quercetin supplementation on testicular functions in summer heat-stressed rabbits. World Rabbit Sci., 28: 19-27. https://doi.org/10.4995/wrs.2020.12420 | es_ES |
dc.description.references | Oliveira R.A, Piersanti R.L., Wolf C.A., Viu M.A.O., Gambarini M.L. 2014. Glutathione for the freezing of cooled equine semen, using different protocols. Anim. Reprod., 11: 104-109. | es_ES |
dc.description.references | Peña F.J., Johannisson A., Wallgren M., Rodriguez-Martinez H. 2003. Antioxidant supplementation in vitro improves boar sperm motility and mitochondrial membrane potential after cryopreservation of different fractions of the ejaculate. Anim. Reprod. Sci., 78: 85-98. | es_ES |
dc.description.references | https://doi.org/10.1016/S0378-4320(03)00049-6 | es_ES |
dc.description.references | Petruska P., Capcarova M., Sutovsky P. 2014. Antioxidant supplementation and purification of semen for improved artificial insemination in livestock species. Turk. J. Vet. Anim. Sci., 38: 643-652. https://doi.org/10.3906/vet-1404-61 | es_ES |
dc.description.references | Prete C.D., Ciani F., Tafuri S., Pasolini M.P., Valle G.D., Palumbo V., Abbondante L., Calamo A., Barbato V., Gualtieri R., Talevi R., Cocchia N. 2018. Effect of superoxide dismutase, catalase, and glutathione peroxidase supplementation in the extender on chilled semen of fertile and hypofertile dogs. J. Vet. Sci., 19: 667-675. https://doi.org/10.4142/jvs.2018.19.5.667 | es_ES |
dc.description.references | Rosato M.P., Di Iorio M., Manchisi A., Gambacorta M., Petrosino G., Centoducati G., Santacroce M. P., Iaffaldano N. 2012. In vitro survival and lipid peroxidation status of rabbit spermatozoa after both chilled and frozen storage in lycopene enriched extenders. Livest. Sci., 146: 199-202. https://doi.org/10.1016/j.livsci.2012.03.006 | es_ES |
dc.description.references | Rosato M.P., Iaffaldano N. 2011. Effect of chilling temperature on the long-term survival of rabbit spermatozoa held either in a trisbased or a jellified extender. Reprod. Dom. Anim., 46: 301-308. https://doi.org/10.1111/j.1439-0531.2010.01667.x | es_ES |
dc.description.references | Sarangi A., Singh P., Virmani M., Yadav A.S., Sahu S., Ajithakumar H.M., Kumari A., Rath A.P. 2017. Effect of antioxidants supplementation on the quality of Beetal buck semen stored at 4°C. Vet. World, 10: 1184-1188. https://doi.org/10.14202/vetworld.2017.1184-1188 | es_ES |
dc.description.references | Saraswat S., Jindal S.K., Kharche S.D. 2016. Antioxidant and spermatozoa: a complex story- A review. Indian J. Anim. Sci., 86: 495-501. | es_ES |
dc.description.references | Sarıözkan S., Özdamar S., Türk G., Cantürk F., Yay A. 2014. In vitro effects of l-carnitine and glutamine on motility, acrosomal abnormality, and plasma membrane integrity of rabbit sperm during liquid-storage. Cryobiology 68: 349-353. https://doi.org/10.1016/j.cryobiol.2014.04.006 | es_ES |
dc.description.references | Sarıözkan S., Türk G., Cantürk F., Yay A., Eken A., Akçay A. 2013. The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology, 67: 1-6. https://doi.org/10.1016/j.cryobiol.2013.04.002 | es_ES |
dc.description.references | Yimer N., Kaka A., Yusoff R., Haron A.W. 2016. The roles of antioxidants and fatty acids in sperm cryopreservation. Cryopreservation in Eukaryotes, Francisco Marco-Jiménez and Hülya Akdemir, IntechOpen. https://doi.org/10.5772/65571 | es_ES |
dc.description.references | Zeitoun M.M., Al-Damegh M.A. 2015. Effect of nonenzymatic antioxidants on sperm motility and survival relative to free radicals and antioxidant enzymes of chilled-stored ram semen. Open J. Anim. Sci., 5: 50-58. https://doi.org/10.4236/ojas.2015.51007 | es_ES |
dc.description.references | Zhandi M., Ghadimi V. 2014. Effect of glutathione-supplemented INRA82 extender on miniature Caspian stallion sperm quality during storage at 5C. J. Equine Vet. Sci., 34: 606-610. https://doi.org/10.1016/j.jevs.2013.11.009 | es_ES |