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Glutathione supplementation in semen extender improves rabbit sperm attributes during refrigeration

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Glutathione supplementation in semen extender improves rabbit sperm attributes during refrigeration

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dc.contributor.author Ahmad, Ejaz es_ES
dc.contributor.author Naseer, Zahid es_ES
dc.contributor.author Aksoy, Melih es_ES
dc.date.accessioned 2021-07-06T07:05:02Z
dc.date.available 2021-07-06T07:05:02Z
dc.date.issued 2021-06-30
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/168816
dc.description.abstract [EN] In the present study, we evaluated the sustaining effect of various glutathione (GSH) concentrations in extender on rabbit sperm attributes during storage at 5°C for 24 h. Semen was collected from regular donor rabbit bucks using an artificial vagina and initially evaluated for sperm quality. The qualifying ejaculates were diluted with one of the extenders having 0, 1, 2, 4 or 8 mM GSH, to achieve a final concentration of 1×108 sperm/mL. The extended samples were stored at 5°C for 24 h. Sperm motility, motion kinetics, acrosome integrity and viability were assessed after 3, 6, 12 and 24 h of storage. The results showed that total sperm motility and sperm motion kinetics (oscillation index of the sperm, straightness index and beat cross frequency) were influenced (P<0.05) by glutathione dose and refrigeration time. An interaction of (P<0.05) GSH concentrations and refrigeration time was observed for sperm viability and acrosome reaction rate. In conclusion, the 4 mM GSH supplemented extender’s protective influence was remarkable to maintainrabbit sperm quality for 24 h 5°C. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València es_ES
dc.relation.ispartof World Rabbit Science es_ES
dc.rights Reconocimiento - No comercial - Compartir igual (by-nc-sa) es_ES
dc.subject Glutathione es_ES
dc.subject Refrigeration es_ES
dc.subject Sperm quality es_ES
dc.subject Rabbit es_ES
dc.title Glutathione supplementation in semen extender improves rabbit sperm attributes during refrigeration es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.4995/wrs.2021.14759
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Ahmad, E.; Naseer, Z.; Aksoy, M. (2021). Glutathione supplementation in semen extender improves rabbit sperm attributes during refrigeration. World Rabbit Science. 29(2):81-86. https://doi.org/10.4995/wrs.2021.14759 es_ES
dc.description.accrualMethod OJS es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2021.14759 es_ES
dc.description.upvformatpinicio 81 es_ES
dc.description.upvformatpfin 86 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 29 es_ES
dc.description.issue 2 es_ES
dc.identifier.eissn 1989-8886
dc.relation.pasarela OJS\14759 es_ES
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