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Effect of embryo vitrification on the steroid biosynthesis of liver tissue in rabbit offspring

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Effect of embryo vitrification on the steroid biosynthesis of liver tissue in rabbit offspring

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dc.contributor.author Marco-Jiménez, Francisco es_ES
dc.contributor.author Garcia-Dominguez, X es_ES
dc.contributor.author Domínguez-Martínez, Marta es_ES
dc.contributor.author Viudes-de-Castro, María Pilar es_ES
dc.contributor.author Diretto, Gianfranco es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.date.accessioned 2021-07-17T03:34:47Z
dc.date.available 2021-07-17T03:34:47Z
dc.date.issued 2020-11 es_ES
dc.identifier.uri http://hdl.handle.net/10251/169422
dc.description.abstract [EN] Preimplantation embryo manipulations during standard assisted reproductive technologies (ART) have significant repercussions on offspring. However, few studies to date have investigated the potential long-term outcomes associated with the vitrification procedure. Here, we performed an experiment to unravel the particular effects related to stress induced by embryo transfer and vitrification techniques on offspring phenotype from the foetal period through to prepuberal age, using a rabbit model. In addition, the focus was extended to the liver function at prepuberal age. We showed that, compared to naturally conceived animals (NC), offspring derived after embryo exposure to the transfer procedure (FT) or cryopreservation-transfer procedure (VT) exhibited variation in growth and body weight from foetal life to prepuberal age. Strikingly, we found a nonlinear relationship between FT and VT stressors, most of which were already present in the FT animals. Furthermore, we displayed evidence of variation in liver function at prepuberal age, most of which occurred in both FT and VT animals. The present major novel finding includes a significant alteration of the steroid biosynthesis profile. In summary, here we provide that embryonic manipulation during the vitrification process is linked with embryo phenotypic adaptation detected from foetal life to prepuberal age and suggests that this phenotypic variation may be associated, to a great extent, with the effect of embryo transfer. es_ES
dc.description.sponsorship This research was funded by Conselleria d'Educacio, Investigacio, Cultura i Esport, Spain, grant number AICO/2019/272. Ximo Garcia-Dominguez was supported by a research grant from the Ministry of Economy, Industry and Competitiveness of Spain (BES-2015-072429). es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof International Journal of Molecular Sciences es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Embryo es_ES
dc.subject Assisted reproductive technologies es_ES
dc.subject Cryopreservation es_ES
dc.subject Stress es_ES
dc.subject Metabolome es_ES
dc.subject Cholesterol es_ES
dc.subject IGF-I es_ES
dc.subject Steroid biosynthesis es_ES
dc.subject RT-qPCR es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.title Effect of embryo vitrification on the steroid biosynthesis of liver tissue in rabbit offspring es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/ijms21228642 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BES-2015-072429/ES/BES-2015-072429/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//AICO%2F2019%2F272/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Marco-Jiménez, F.; Garcia-Dominguez, X.; Domínguez-Martínez, M.; Viudes-De-Castro, MP.; Diretto, G.; Peñaranda, D.; Vicente Antón, JS. (2020). Effect of embryo vitrification on the steroid biosynthesis of liver tissue in rabbit offspring. International Journal of Molecular Sciences. 21(22):1-17. https://doi.org/10.3390/ijms21228642 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/ijms21228642 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 17 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 21 es_ES
dc.description.issue 22 es_ES
dc.identifier.eissn 1422-0067 es_ES
dc.identifier.pmid 33207830 es_ES
dc.identifier.pmcid PMC7696440 es_ES
dc.relation.pasarela S\424925 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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