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Extended metabolic biosensor design for dynamic pathway regulation of cell factories

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Extended metabolic biosensor design for dynamic pathway regulation of cell factories

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dc.contributor.author Boada-Acosta, Yadira Fernanda es_ES
dc.contributor.author Vignoni, Alejandro es_ES
dc.contributor.author Picó, Jesús es_ES
dc.contributor.author Carbonell, Pablo es_ES
dc.date.accessioned 2021-07-22T03:33:46Z
dc.date.available 2021-07-22T03:33:46Z
dc.date.issued 2020-07-24 es_ES
dc.identifier.uri http://hdl.handle.net/10251/169743
dc.description.abstract [EN] Transcription factor-based biosensors naturally occur in metabolic pathways to maintain cell growth and to provide a robust response to environmental fluctua-tions. Extended metabolic biosensors, i.e., the cascading of a bio-conversion pathway and a transcription factor (TF) responsive to the downstream effector metabolite, provide sensing capabilities beyond natural effectors for implement-ing context-aware synthetic genetic circuits and bio-observers. However, the engineering of such multi-step circuits is challenged by stability and robustness issues. In order to streamline the design of TF-based biosensors in metabolic pathways, here we investigate the response of a genetic circuit combining a TF-based extended metabolic biosensor with an antithetic integral circuit, a feed-back controller that achieves robustness against environmental fluctuations. The dynamic response of an extended biosensor-based regulated flavonoid pathway is analyzed in order to address the issues of biosensor tuning of the regulated pathway under industrial biomanufacturing operating constraints. es_ES
dc.description.sponsorship This work is partially supported by grant MINECO/AEI and EU DPI2017-82896-C2-1-R. P.C. acknowledges support from the Universitat Politecnica de Valencia Talento Programme. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier (Cell Press) es_ES
dc.relation.ispartof iScience es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject.classification INGENIERIA DE SISTEMAS Y AUTOMATICA es_ES
dc.title Extended metabolic biosensor design for dynamic pathway regulation of cell factories es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.isci.2020.101305 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/DPI2017-82896-C2-1-R/ES/DISEÑO, CARACTERIZACION Y AJUSTE OPTIMO DE BIOCIRCUITOS SINTETICOS PARA BIOPRODUCCION CON CONTROL DE CARGA METABOLICA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ingeniería de Sistemas y Automática - Departament d'Enginyeria de Sistemes i Automàtica es_ES
dc.description.bibliographicCitation Boada-Acosta, YF.; Vignoni, A.; Picó, J.; Carbonell, P. (2020). Extended metabolic biosensor design for dynamic pathway regulation of cell factories. iScience. 23(7):1-25. https://doi.org/10.1016/j.isci.2020.101305 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.isci.2020.101305 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 25 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 23 es_ES
dc.description.issue 7 es_ES
dc.identifier.eissn 2589-0042 es_ES
dc.identifier.pmid 32629420 es_ES
dc.identifier.pmcid PMC7334618 es_ES
dc.relation.pasarela S\414464 es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
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dc.subject.ods 08.- Fomentar el crecimiento económico sostenido, inclusivo y sostenible, el empleo pleno y productivo, y el trabajo decente para todos es_ES


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