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Gibberellin-mediated RGA-LIKE1 degradation regulates embryo sac development in Arabidopsis

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Gibberellin-mediated RGA-LIKE1 degradation regulates embryo sac development in Arabidopsis

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dc.contributor.author Gomez, Maria Dolores es_ES
dc.contributor.author Barro-Trastoy, Daniela es_ES
dc.contributor.author Fuster Almunia, Clara es_ES
dc.contributor.author Tornero Feliciano, Pablo es_ES
dc.contributor.author Alonso, Jose M. es_ES
dc.contributor.author PEREZ AMADOR, MIGUEL ANGEL es_ES
dc.date.accessioned 2021-07-23T03:31:11Z
dc.date.available 2021-07-23T03:31:11Z
dc.date.issued 2020-12-31 es_ES
dc.identifier.issn 0022-0957 es_ES
dc.identifier.uri http://hdl.handle.net/10251/169899
dc.description.abstract [EN] Ovule development is essential for plant survival, as it allows correct embryo and seed development upon fertilization. The female gametophyte is formed in the central area of the nucellus during ovule development, in a complex developmental programme that involves key regulatory genes and the plant hormones auxins and brassinosteroids. Here we provide novel evidence of the role of gibberellins (GAs) in the control of megagametogenesis and embryo sac development, via the GA-dependent degradation of RGA-LIKE1 (RGL1) in the ovule primordia. YPet-rgl1.17 plants, which express a dominant version of RGL1, showed reduced fertility, mainly due to altered embryo sac formation that varied from partial to total ablation. YPet-rgl1.17 ovules followed normal development of the megaspore mother cell, meiosis, and formation of the functional megaspore, but YPet-rgl1.17 plants had impaired mitotic divisions of the functional megaspore. This phenotype is RGL1-specific, as it is not observed in any other dominant mutants of the DELLA proteins. Expression analysis of YPet-rgl1.17 coupled to in situ localization of bioactive GAs in ovule primordia led us to propose a mechanism of GA-mediated RGL1 degradation that allows proper embryo sac development. Taken together, our data unravel a novel specific role of GAs in the control of female gametophyte development. es_ES
dc.description.sponsorship We wish to thank the IBMCP microscopy facility, and Ms J. Yun for technical assistance. We also thank Jennifer Nemhauser (University of Washington, USA) for the HACR sensor. Cambridge proofreading (https://proofreading.org/order/) provided proofreading and editing of this manuscript. This work was supported by grants from the Spanish Ministry for Science and Innovation-FEDER [BIO2017-83138R] to MAP-A and National Science Foundation [MCB-0923727] to JMA. MAP-A received a fellowship of the `Salvador de Madariaga' program from Spanish Ministry of Science and Innovation. We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Journal of Experimental Botany es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Arabidopsis es_ES
dc.subject DELLA es_ES
dc.subject Development es_ES
dc.subject Embryo sac es_ES
dc.subject Gibberellin es_ES
dc.subject Megagametogenesis es_ES
dc.subject Ovule es_ES
dc.subject RGL1 es_ES
dc.title Gibberellin-mediated RGA-LIKE1 degradation regulates embryo sac development in Arabidopsis es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/jxb/eraa395 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//0923727/US/Molecular Genetics of Ethylene-auxin Interactions in Arabidopsis/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83138-R/ES/LAS GIBERELINAS EN EL CONTROL DE LA FORMACION DE OVULOS Y SEMILLAS: DISEÑO DE HERRAMIENTAS PARA LA MEJORA VEGETAL/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Gomez, MD.; Barro-Trastoy, D.; Fuster Almunia, C.; Tornero Feliciano, P.; Alonso, JM.; Perez Amador, MA. (2020). Gibberellin-mediated RGA-LIKE1 degradation regulates embryo sac development in Arabidopsis. Journal of Experimental Botany. 71(22):7059-7072. https://doi.org/10.1093/jxb/eraa395 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/jxb/eraa395 es_ES
dc.description.upvformatpinicio 7059 es_ES
dc.description.upvformatpfin 7072 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 71 es_ES
dc.description.issue 22 es_ES
dc.identifier.pmid 32845309 es_ES
dc.identifier.pmcid PMC7906783 es_ES
dc.relation.pasarela S\433419 es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder National Science Foundation, EEUU es_ES
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