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Poly(vinylidene) fluoride membranes coated by heparin/collagen layer-by-layer, smart biomimetic approaches for mesenchymal stem cell culture

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Poly(vinylidene) fluoride membranes coated by heparin/collagen layer-by-layer, smart biomimetic approaches for mesenchymal stem cell culture

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dc.contributor.author Guillot-Ferriols, María Teresa es_ES
dc.contributor.author Rodriguez-Hernandez, Jose-Carlos es_ES
dc.contributor.author Correia, D.M. es_ES
dc.contributor.author Carabineiro, S.A.C. es_ES
dc.contributor.author Lanceros-Méndez, S. es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Gallego-Ferrer, Gloria es_ES
dc.date.accessioned 2021-09-09T03:35:18Z
dc.date.available 2021-09-09T03:35:18Z
dc.date.issued 2020-12 es_ES
dc.identifier.uri http://hdl.handle.net/10251/171684
dc.description.abstract [EN] The use of piezoelectric materials in tissue engineering has grown considerably since inherent bone piezoelectricity was discovered. Combinations of piezoelectric polymers with magnetostrictive nanoparticles (MNP) can be used to magnetoelectrically stimulate cells by applying an external magnetic field which deforms the magnetostrictive nanoparticles in the polymer matrix, deforming the polymer itself, which varies the surface charge due to the piezoelectric effect. Poly(vinylidene) fluoride (PVDF) is the piezoelectric polymer with the largest piezoelectric coefficients, being a perfect candidate for osteogenic differentiation. As a first approach, in this paper, we propose PVDF membranes containing magnetostrictive nanoparticles and a biomimetic heparin/ collagen layer-by-layer (LbL) coating for mesenchymal stem cell culture. PVDF membranes 20% (w/v) with and without cobalt ferrite oxide (PVDF-CFO) 10% (w/w) were produced by non-solvent induced phase separation (NIPS). These membranes were found to be asymmetric, with a smooth surface, crystallinity ranging from 65% to 61%, and an electroactive beta-phase content of 51.8% and 55.6% for PVDF and PVDF-CFO, respectively. Amine groups were grafted onto the membrane surface by an alkali treatment, confirmed by ninhydrin test and X-ray photoelectron spectroscopy (XPS), providing positive charges for the assembly of heparin/collagen layers by the LbL technique. Five layers of each polyelectrolyte were deposited, ending with collagen. Human mesenchymal stem cells (hMSC) were used to test cell response in a short-term culture (1, 3 and 7 days). Nucleus cell counting showed that LbL favored cell proliferation in PVDF-CFO over non-coated membranes. es_ES
dc.description.sponsorship This work has been funded by the Spanish State Research Agency (AEI) and the European Regional Development Fund (ERFD) through the PID2019-106099RB-C41/AEI/10.13039/501100011033 and PID2019-106099RB-C43/AEI/10.13039/501100011033 projects and the Associate Laboratory for Green Chemistry-LAQV financed by national funds from FCT/MCTES (UIDB/50006/2020). Maria GuillotFerriols acknowledges the Spanish Government funding of her doctoral thesis through a BES-2017-080398 FPI Grant. The CIBER-BBN (CB06/01/1026) initiative is funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. D.M.C is also grateful to the FCT-Fundacao para a Ciencia e Tecnologia for grant SFRH/BPD/121526/2016. Finally, the authors acknowledge funding from the Basque Government Industry and Education Department under the ELKARTEK, HAZITEK and PIBA (PIBA-2018-06) programs, respectively, also Dr. Carlos Sa (CEMUP) for assistance with the XPS analyses. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier BV es_ES
dc.relation.ispartof Materials Science and Engineering C: Materials for Biological Applications (Online) es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Poly(vinylidene) fluoride es_ES
dc.subject Non-solvent induced phase separation es_ES
dc.subject Layer-by-layer es_ES
dc.subject Collagen es_ES
dc.subject Mesenchymal stem cells es_ES
dc.subject Piezoelectricity es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.title Poly(vinylidene) fluoride membranes coated by heparin/collagen layer-by-layer, smart biomimetic approaches for mesenchymal stem cell culture es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.msec.2020.111281 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F121526%2F2016/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//UIDB%2F50006%2F2020/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MSC//CB06%2F01%2F1026/ES/Desarrollo e implementación de nuevas tecnologías en biomedicina 106/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Eusko Jaurlaritza//PIBA-2018-06/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT/3599-PPCDT/121526/PT/Heterometallic Metal-organic Frameworks: Smart Materials for Advanced Applications/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106099RB-C43/ES/DESARROLLO DE ANDAMIAJES BIOMIMETICOS ACTIVOS PARA EL ESTUDIO DE MICROENTORNO DE TUMOR EN OSTEOSARCOMA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//BES-2017-080398/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106099RB-C41/ES/MICROGELES BIOMIMETICOS PARA EL ESTUDIO DE LA GENERACION DE RESISTENCIAS A FARMACOS EN EL MIELOMA MULTIPLE./ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Guillot-Ferriols, MT.; Rodriguez-Hernandez, J.; Correia, D.; Carabineiro, S.; Lanceros-Méndez, S.; Gómez Ribelles, JL.; Gallego-Ferrer, G. (2020). Poly(vinylidene) fluoride membranes coated by heparin/collagen layer-by-layer, smart biomimetic approaches for mesenchymal stem cell culture. Materials Science and Engineering C: Materials for Biological Applications (Online). 117:1-12. https://doi.org/10.1016/j.msec.2020.111281 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.msec.2020.111281 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 117 es_ES
dc.identifier.eissn 1873-0191 es_ES
dc.identifier.pmid 32919642 es_ES
dc.relation.pasarela S\416553 es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Gobierno Vasco/Eusko Jaurlaritza es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Fundação para a Ciência e a Tecnologia, Portugal es_ES
dc.contributor.funder Ministerio de Sanidad y Consumo
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