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Poly(vinylidene) fluoride membranes coated by heparin/collagen layer-by-layer, smart biomimetic approaches for mesenchymal stem cell culture

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Poly(vinylidene) fluoride membranes coated by heparin/collagen layer-by-layer, smart biomimetic approaches for mesenchymal stem cell culture

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dc.contributor.author Guillot-Ferriols, María Teresa es_ES
dc.contributor.author Rodriguez-Hernandez, Jose-Carlos es_ES
dc.contributor.author Correia, D.M. es_ES
dc.contributor.author Carabineiro, S.A.C. es_ES
dc.contributor.author Lanceros-Méndez, S. es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Gallego-Ferrer, Gloria es_ES
dc.date.accessioned 2021-09-09T03:35:18Z
dc.date.available 2021-09-09T03:35:18Z
dc.date.issued 2020-12 es_ES
dc.identifier.uri http://hdl.handle.net/10251/171684
dc.description.abstract [EN] The use of piezoelectric materials in tissue engineering has grown considerably since inherent bone piezoelectricity was discovered. Combinations of piezoelectric polymers with magnetostrictive nanoparticles (MNP) can be used to magnetoelectrically stimulate cells by applying an external magnetic field which deforms the magnetostrictive nanoparticles in the polymer matrix, deforming the polymer itself, which varies the surface charge due to the piezoelectric effect. Poly(vinylidene) fluoride (PVDF) is the piezoelectric polymer with the largest piezoelectric coefficients, being a perfect candidate for osteogenic differentiation. As a first approach, in this paper, we propose PVDF membranes containing magnetostrictive nanoparticles and a biomimetic heparin/ collagen layer-by-layer (LbL) coating for mesenchymal stem cell culture. PVDF membranes 20% (w/v) with and without cobalt ferrite oxide (PVDF-CFO) 10% (w/w) were produced by non-solvent induced phase separation (NIPS). These membranes were found to be asymmetric, with a smooth surface, crystallinity ranging from 65% to 61%, and an electroactive beta-phase content of 51.8% and 55.6% for PVDF and PVDF-CFO, respectively. Amine groups were grafted onto the membrane surface by an alkali treatment, confirmed by ninhydrin test and X-ray photoelectron spectroscopy (XPS), providing positive charges for the assembly of heparin/collagen layers by the LbL technique. Five layers of each polyelectrolyte were deposited, ending with collagen. Human mesenchymal stem cells (hMSC) were used to test cell response in a short-term culture (1, 3 and 7 days). Nucleus cell counting showed that LbL favored cell proliferation in PVDF-CFO over non-coated membranes. es_ES
dc.description.sponsorship This work has been funded by the Spanish State Research Agency (AEI) and the European Regional Development Fund (ERFD) through the PID2019-106099RB-C41/AEI/10.13039/501100011033 and PID2019-106099RB-C43/AEI/10.13039/501100011033 projects and the Associate Laboratory for Green Chemistry-LAQV financed by national funds from FCT/MCTES (UIDB/50006/2020). Maria GuillotFerriols acknowledges the Spanish Government funding of her doctoral thesis through a BES-2017-080398 FPI Grant. The CIBER-BBN (CB06/01/1026) initiative is funded by the VI National R&D&I Plan 2008-2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. D.M.C is also grateful to the FCT-Fundacao para a Ciencia e Tecnologia for grant SFRH/BPD/121526/2016. Finally, the authors acknowledge funding from the Basque Government Industry and Education Department under the ELKARTEK, HAZITEK and PIBA (PIBA-2018-06) programs, respectively, also Dr. Carlos Sa (CEMUP) for assistance with the XPS analyses. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier BV es_ES
dc.relation.ispartof Materials Science and Engineering C: Materials for Biological Applications (Online) es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Poly(vinylidene) fluoride es_ES
dc.subject Non-solvent induced phase separation es_ES
dc.subject Layer-by-layer es_ES
dc.subject Collagen es_ES
dc.subject Mesenchymal stem cells es_ES
dc.subject Piezoelectricity es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.title Poly(vinylidene) fluoride membranes coated by heparin/collagen layer-by-layer, smart biomimetic approaches for mesenchymal stem cell culture es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.msec.2020.111281 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F121526%2F2016/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT//UIDB%2F50006%2F2020/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MSC//CB06%2F01%2F1026/ES/Desarrollo e implementación de nuevas tecnologías en biomedicina 106/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Eusko Jaurlaritza//PIBA-2018-06/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/FCT/3599-PPCDT/121526/PT/Heterometallic Metal-organic Frameworks: Smart Materials for Advanced Applications/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106099RB-C43/ES/DESARROLLO DE ANDAMIAJES BIOMIMETICOS ACTIVOS PARA EL ESTUDIO DE MICROENTORNO DE TUMOR EN OSTEOSARCOMA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//BES-2017-080398/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106099RB-C41/ES/MICROGELES BIOMIMETICOS PARA EL ESTUDIO DE LA GENERACION DE RESISTENCIAS A FARMACOS EN EL MIELOMA MULTIPLE./ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Guillot-Ferriols, MT.; Rodriguez-Hernandez, J.; Correia, D.; Carabineiro, S.; Lanceros-Méndez, S.; Gómez Ribelles, JL.; Gallego-Ferrer, G. (2020). Poly(vinylidene) fluoride membranes coated by heparin/collagen layer-by-layer, smart biomimetic approaches for mesenchymal stem cell culture. Materials Science and Engineering C: Materials for Biological Applications (Online). 117:1-12. https://doi.org/10.1016/j.msec.2020.111281 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.msec.2020.111281 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 117 es_ES
dc.identifier.eissn 1873-0191 es_ES
dc.identifier.pmid 32919642 es_ES
dc.relation.pasarela S\416553 es_ES
dc.contributor.funder Instituto de Salud Carlos III es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Gobierno Vasco/Eusko Jaurlaritza es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Fundação para a Ciência e a Tecnologia, Portugal es_ES
dc.contributor.funder Ministerio de Sanidad y Consumo
dc.description.references Jacob, J., More, N., Kalia, K., & Kapusetti, G. (2018). Piezoelectric smart biomaterials for bone and cartilage tissue engineering. Inflammation and Regeneration, 38(1). doi:10.1186/s41232-018-0059-8 es_ES
dc.description.references Fukada, E., & Yasuda, I. (1957). On the Piezoelectric Effect of Bone. Journal of the Physical Society of Japan, 12(10), 1158-1162. doi:10.1143/jpsj.12.1158 es_ES
dc.description.references Martins, P., Lopes, A. C., & Lanceros-Mendez, S. (2014). Electroactive phases of poly(vinylidene fluoride): Determination, processing and applications. Progress in Polymer Science, 39(4), 683-706. doi:10.1016/j.progpolymsci.2013.07.006 es_ES
dc.description.references Gregorio, R. (2006). Determination of the α, β, and γ crystalline phases of poly(vinylidene fluoride) films prepared at different conditions. Journal of Applied Polymer Science, 100(4), 3272-3279. doi:10.1002/app.23137 es_ES
dc.description.references Sencadas, V., Gregorio, R., & Lanceros-Méndez, S. (2009). α to β Phase Transformation and Microestructural Changes of PVDF Films Induced by Uniaxial Stretch. Journal of Macromolecular Science, Part B, 48(3), 514-525. doi:10.1080/00222340902837527 es_ES
dc.description.references Gregorio, R., & Borges, D. S. (2008). Effect of crystallization rate on the formation of the polymorphs of solution cast poly(vinylidene fluoride). Polymer, 49(18), 4009-4016. doi:10.1016/j.polymer.2008.07.010 es_ES
dc.description.references Sencadas, V., Gregorio Filho, R., & Lanceros-Mendez, S. (2006). Processing and characterization of a novel nonporous poly(vinilidene fluoride) films in the β phase. Journal of Non-Crystalline Solids, 352(21-22), 2226-2229. doi:10.1016/j.jnoncrysol.2006.02.052 es_ES
dc.description.references Buonomenna, M. G., Macchi, P., Davoli, M., & Drioli, E. (2007). Poly(vinylidene fluoride) membranes by phase inversion: the role the casting and coagulation conditions play in their morphology, crystalline structure and properties. European Polymer Journal, 43(4), 1557-1572. doi:10.1016/j.eurpolymj.2006.12.033 es_ES
dc.description.references Ribeiro, C., Costa, C. M., Correia, D. M., Nunes-Pereira, J., Oliveira, J., Martins, P., … Lanceros-Méndez, S. (2018). Electroactive poly(vinylidene fluoride)-based structures for advanced applications. Nature Protocols, 13(4), 681-704. doi:10.1038/nprot.2017.157 es_ES
dc.description.references Liu, F., Hashim, N. A., Liu, Y., Abed, M. R. M., & Li, K. (2011). Progress in the production and modification of PVDF membranes. Journal of Membrane Science, 375(1-2), 1-27. doi:10.1016/j.memsci.2011.03.014 es_ES
dc.description.references Abzan, N., Kharaziha, M., & Labbaf, S. (2019). Development of three-dimensional piezoelectric polyvinylidene fluoride-graphene oxide scaffold by non-solvent induced phase separation method for nerve tissue engineering. Materials & Design, 167, 107636. doi:10.1016/j.matdes.2019.107636 es_ES
dc.description.references Young, T.-H., Chang, H.-H., Lin, D.-J., & Cheng, L.-P. (2010). Surface modification of microporous PVDF membranes for neuron culture. Journal of Membrane Science, 350(1-2), 32-41. doi:10.1016/j.memsci.2009.12.009 es_ES
dc.description.references Gonçalves, R., Martins, P., Correia, D. M., Sencadas, V., Vilas, J. L., León, L. M., … Lanceros-Méndez, S. (2015). Development of magnetoelectric CoFe2O4 /poly(vinylidene fluoride) microspheres. RSC Advances, 5(45), 35852-35857. doi:10.1039/c5ra04409j es_ES
dc.description.references Fernandes, M. M., Correia, D. M., Ribeiro, C., Castro, N., Correia, V., & Lanceros-Mendez, S. (2019). Bioinspired Three-Dimensional Magnetoactive Scaffolds for Bone Tissue Engineering. ACS Applied Materials & Interfaces, 11(48), 45265-45275. doi:10.1021/acsami.9b14001 es_ES
dc.description.references Hermenegildo, B., Ribeiro, C., Pérez-Álvarez, L., Vilas, J. L., Learmonth, D. A., Sousa, R. A., … Lanceros-Méndez, S. (2019). Hydrogel-based magnetoelectric microenvironments for tissue stimulation. Colloids and Surfaces B: Biointerfaces, 181, 1041-1047. doi:10.1016/j.colsurfb.2019.06.023 es_ES
dc.description.references Gonçalves, R., Martins, P., Moya, X., Ghidini, M., Sencadas, V., Botelho, G., … Lanceros-Mendez, S. (2015). Magnetoelectric CoFe2O4/polyvinylidene fluoride electrospun nanofibres. Nanoscale, 7(17), 8058-8061. doi:10.1039/c5nr00453e es_ES
dc.description.references Silva, J. M., Reis, R. L., & Mano, J. F. (2016). Biomimetic Extracellular Environment Based on Natural Origin Polyelectrolyte Multilayers. Small, 12(32), 4308-4342. doi:10.1002/smll.201601355 es_ES
dc.description.references Costa, R. R., & Mano, J. F. (2014). Polyelectrolyte multilayered assemblies in biomedical technologies. Chemical Society Reviews, 43(10), 3453. doi:10.1039/c3cs60393h es_ES
dc.description.references Castilla-Casadiego, D. A., Pinzon-Herrera, L., Perez-Perez, M., Quiñones-Colón, B. A., Suleiman, D., & Almodovar, J. (2018). Simultaneous characterization of physical, chemical, and thermal properties of polymeric multilayers using infrared spectroscopic ellipsometry. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 553, 155-168. doi:10.1016/j.colsurfa.2018.05.052 es_ES
dc.description.references Mhanna, R. F., Vörös, J., & Zenobi-Wong, M. (2011). Layer-by-Layer Films Made from Extracellular Matrix Macromolecules on Silicone Substrates. Biomacromolecules, 12(3), 609-616. doi:10.1021/bm1012772 es_ES
dc.description.references Billings, P. C., & Pacifici, M. (2015). Interactions of signaling proteins, growth factors and other proteins with heparan sulfate: mechanisms and mysteries. Connective Tissue Research, 56(4), 272-280. doi:10.3109/03008207.2015.1045066 es_ES
dc.description.references Chen, J., Huang, N., Li, Q., Chu, C. H., Li, J., & Maitz, M. F. (2016). The effect of electrostatic heparin/collagen layer-by-layer coating degradation on the biocompatibility. Applied Surface Science, 362, 281-289. doi:10.1016/j.apsusc.2015.11.227 es_ES
dc.description.references Zhang, K., Huang, D., Yan, Z., & Wang, C. (2017). Heparin/collagen encapsulating nerve growth factor multilayers coated aligned PLLA nanofibrous scaffolds for nerve tissue engineering. Journal of Biomedical Materials Research Part A, 105(7), 1900-1910. doi:10.1002/jbm.a.36053 es_ES
dc.description.references Ferreira, A. M., Gentile, P., Toumpaniari, S., Ciardelli, G., & Birch, M. A. (2016). Impact of Collagen/Heparin Multilayers for Regulating Bone Cellular Functions. ACS Applied Materials & Interfaces, 8(44), 29923-29932. doi:10.1021/acsami.6b09241 es_ES
dc.description.references Castilla-Casadiego, D. A., García, J. R., García, A. J., & Almodovar, J. (2019). Heparin/Collagen Coatings Improve Human Mesenchymal Stromal Cell Response to Interferon Gamma. ACS Biomaterials Science & Engineering, 5(6), 2793-2803. doi:10.1021/acsbiomaterials.9b00008 es_ES
dc.description.references Martins, P., Gonçalves, R., Lanceros-Mendez, S., Lasheras, A., Gutiérrez, J., & Barandiarán, J. M. (2014). Effect of filler dispersion and dispersion method on the piezoelectric and magnetoelectric response of CoFe2O4/P(VDF-TrFE) nanocomposites. Applied Surface Science, 313, 215-219. doi:10.1016/j.apsusc.2014.05.187 es_ES
dc.description.references Gamboa-Martínez, T. C., Luque-Guillén, V., González-García, C., Gómez Ribelles, J. L., & Gallego-Ferrer, G. (2014). Crosslinked fibrin gels for tissue engineering: Two approaches to improve their properties. Journal of Biomedical Materials Research Part A, 103(2), 614-621. doi:10.1002/jbm.a.35210 es_ES
dc.description.references Gregorio, Jr., R., & Cestari, M. (1994). Effect of crystallization temperature on the crystalline phase content and morphology of poly(vinylidene fluoride). Journal of Polymer Science Part B: Polymer Physics, 32(5), 859-870. doi:10.1002/polb.1994.090320509 es_ES
dc.description.references Martins, P., Costa, C. M., & Lanceros-Mendez, S. (2010). Nucleation of electroactive β-phase poly(vinilidene fluoride) with CoFe2O4 and NiFe2O4 nanofillers: a new method for the preparation of multiferroic nanocomposites. Applied Physics A, 103(1), 233-237. doi:10.1007/s00339-010-6003-7 es_ES
dc.description.references Qi, L., Knapton, E. K., Zhang, X., Zhang, T., Gu, C., & Zhao, Y. (2017). Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds. Scientific Reports, 7(1). doi:10.1038/s41598-017-08723-2 es_ES
dc.description.references Young, T.-H., Cheng, L.-P., Lin, D.-J., Fane, L., & Chuang, W.-Y. (1999). Mechanisms of PVDF membrane formation by immersion-precipitation in soft (1-octanol) and harsh (water) nonsolvents. Polymer, 40(19), 5315-5323. doi:10.1016/s0032-3861(98)00747-2 es_ES
dc.description.references Cheng, L.-P. (1999). Effect of Temperature on the Formation of Microporous PVDF Membranes by Precipitation from 1-Octanol/DMF/PVDF and Water/DMF/PVDF Systems. Macromolecules, 32(20), 6668-6674. doi:10.1021/ma990418l es_ES
dc.description.references Supriya, S., Kumar, L., & Kar, M. (2018). Optimization of dielectric properties of PVDF-CFO nanocomposites. Polymer Composites, 40(3), 1239-1250. doi:10.1002/pc.24840 es_ES
dc.description.references Lin, D.-J., Beltsios, K., Young, T.-H., Jeng, Y.-S., & Cheng, L.-P. (2006). Strong effect of precursor preparation on the morphology of semicrystalline phase inversion poly(vinylidene fluoride) membranes. Journal of Membrane Science, 274(1-2), 64-72. doi:10.1016/j.memsci.2005.07.043 es_ES
dc.description.references Cai, X., Lei, T., Sun, D., & Lin, L. (2017). A critical analysis of the α, β and γ phases in poly(vinylidene fluoride) using FTIR. RSC Advances, 7(25), 15382-15389. doi:10.1039/c7ra01267e es_ES
dc.description.references Boccaccio, T., Bottino, A., Capannelli, G., & Piaggio, P. (2002). Characterization of PVDF membranes by vibrational spectroscopy. Journal of Membrane Science, 210(2), 315-329. doi:10.1016/s0376-7388(02)00407-6 es_ES
dc.description.references Liu, J., Lu, X., & Wu, C. (2013). Effect of Preparation Methods on Crystallization Behavior and Tensile Strength of Poly(vinylidene fluoride) Membranes. Membranes, 3(4), 389-405. doi:10.3390/membranes3040389 es_ES
dc.description.references ZHANG, M., ZHANG, A., ZHU, B., DU, C., & XU, Y. (2008). Polymorphism in porous poly(vinylidene fluoride) membranes formed via immersion precipitation process. Journal of Membrane Science, 319(1-2), 169-175. doi:10.1016/j.memsci.2008.03.029 es_ES
dc.description.references Xiao, L., Davenport, D. M., Ormsbee, L., & Bhattacharyya, D. (2015). Polymerization and Functionalization of Membrane Pores for Water Related Applications. Industrial & Engineering Chemistry Research, 54(16), 4174-4182. doi:10.1021/ie504149t es_ES
dc.description.references Duca, M. D., Plosceanu, C. L., & Pop, T. (1998). Effect of X-rays on poly(vinylidene fluoride) in X-ray photoelectron spectroscopy. Journal of Applied Polymer Science, 67(13), 2125-2129. doi:10.1002/(sici)1097-4628(19980328)67:13<2125::aid-app2>3.0.co;2-g es_ES
dc.description.references Correia, D. M., Ribeiro, C., Sencadas, V., Botelho, G., Carabineiro, S. A. C., Ribelles, J. L. G., & Lanceros-Méndez, S. (2015). Influence of oxygen plasma treatment parameters on poly(vinylidene fluoride) electrospun fiber mats wettability. Progress in Organic Coatings, 85, 151-158. doi:10.1016/j.porgcoat.2015.03.019 es_ES
dc.description.references Kehrer, M., Duchoslav, J., Hinterreiter, A., Cobet, M., Mehic, A., Stehrer, T., & Stifter, D. (2019). XPS investigation on the reactivity of surface imine groups with TFAA. Plasma Processes and Polymers, 16(4), 1800160. doi:10.1002/ppap.201800160 es_ES
dc.description.references Morales-Román, R. M., Guillot-Ferriols, M., Roig-Pérez, L., Lanceros-Mendez, S., Gallego-Ferrer, G., & Gómez Ribelles, J. L. (2019). Freeze-extraction microporous electroactive supports for cell culture. European Polymer Journal, 119, 531-540. doi:10.1016/j.eurpolymj.2019.07.011 es_ES
dc.description.references Camacho, N. P., West, P., Torzilli, P. A., & Mendelsohn, R. (2000). FTIR microscopic imaging of collagen and proteoglycan in bovine cartilage. Biopolymers, 62(1), 1-8. doi:10.1002/1097-0282(2001)62:1<1::aid-bip10>3.0.co;2-o es_ES
dc.description.references Ribeiro, C., Panadero, J. A., Sencadas, V., Lanceros-Méndez, S., Tamaño, M. N., Moratal, D., … Gómez Ribelles, J. L. (2012). Fibronectin adsorption and cell response on electroactive poly(vinylidene fluoride) films. Biomedical Materials, 7(3), 035004. doi:10.1088/1748-6041/7/3/035004 es_ES
dc.description.references Ribeiro, C., Pärssinen, J., Sencadas, V., Correia, V., Miettinen, S., Hytönen, V. P., & Lanceros‐Méndez, S. (2014). Dynamic piezoelectric stimulation enhances osteogenic differentiation of human adipose stem cells. Journal of Biomedical Materials Research Part A, 103(6), 2172-2175. doi:10.1002/jbm.a.35368 es_ES
dc.description.references Sobreiro-Almeida, R., Tamaño-Machiavello, M., Carvalho, E., Cordón, L., Doria, S., Senent, L., … Sempere, A. (2017). Human Mesenchymal Stem Cells Growth and Osteogenic Differentiation on Piezoelectric Poly(vinylidene fluoride) Microsphere Substrates. International Journal of Molecular Sciences, 18(11), 2391. doi:10.3390/ijms18112391 es_ES
dc.description.references Moise, S., Céspedes, E., Soukup, D., Byrne, J. M., El Haj, A. J., & Telling, N. D. (2017). The cellular magnetic response and biocompatibility of biogenic zinc- and cobalt-doped magnetite nanoparticles. Scientific Reports, 7(1). doi:10.1038/srep39922 es_ES


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