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Contamination of pig carcass with Salmonella enterica serovar Typhimurium monophasic variant 1,4 [5], 12: i:- originates mainly in live animals

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Contamination of pig carcass with Salmonella enterica serovar Typhimurium monophasic variant 1,4 [5], 12: i:- originates mainly in live animals

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dc.contributor.author Marin, Clara es_ES
dc.contributor.author Chinillac, Mª Carmen es_ES
dc.contributor.author Cerda-Cuellar, Marta es_ES
dc.contributor.author Montoro-Dasí, Laura es_ES
dc.contributor.author Sevilla-Navarro, Sandra es_ES
dc.contributor.author Marco-Jiménez, Francisco es_ES
dc.contributor.author Ayats, Teresa es_ES
dc.contributor.author Vega, Santiago es_ES
dc.date.accessioned 2021-09-09T03:35:24Z
dc.date.available 2021-09-09T03:35:24Z
dc.date.issued 2020-02-10 es_ES
dc.identifier.issn 0048-9697 es_ES
dc.identifier.uri http://hdl.handle.net/10251/171685
dc.description.abstract [EN] Pork is considered a major source of Salmonella Typhimurium infection in humans in the EU, including monophasic strains (mST). Widespread distribution of virulent serotypes such as monophasic variants of S. Typhimurium have emerged as a public health threat. Despite the current situation, within the EU there is no mandatory programme for the control of Salmonella at pork production level. In this con- text, the aims of this study were: to examine the presence of Salmonella in the swine production system from arrival at the slaughterhouse until the end of processing, and investigate the genetic relationship among serovars. A total of 21 pig herds were intensively sampled during processing at the slaughter- house. ERIC-PCR followed by PFGE were performed among isolates recovered at the different steps in the slaughterhouse to assess their genetic relationship. The results showed a high level of Salmonella pork batch contamination upon arrival at the slaughterhouse (71.4%) and at the end of the slaughtering pro- cess (66.7%), with mST the main serovar isolated from both origins (53.1% and 38.2%, respectively). Similarly, this study shows that 14.3% of the strains isolated from carcasses have the same Xbal-PFGE pro- file as those previously recovered in the slaughterhouse environment, but not in the live animals from that same batch. In conclusion, there is a high level of Salmonella swine batch contamination upon arrival at the slaughterhouse and at the end of the slaughtering process, mST being the most frequently isolated serovar. Moreover, a strong genetic relationship has been observed between strains isolated from the batch on arrival at the slaughterhouse, the processing environment and pork carcass contamination. In this sense, it would be necessary to implement a control programme to reduce the bacterium from pork farms and raise the awareness of biosecurity measures. es_ES
dc.description.sponsorship We wish to thank the slaughterhouse staff for their collaboration in the study and their technical support. In addition, we want to thank University CEU-UCH for the financial support (Consolidacion de Indicadores INDI 18/19 and IDOC 18/12). CERCA Programme from the Generalitat de Catalunya is also acknowledged. The English text version was revised by N. Macowan English Language Service. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Science of The Total Environment es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Pork es_ES
dc.subject MST es_ES
dc.subject PFGE es_ES
dc.subject ERIC-PCR es_ES
dc.subject Slaughterhouse es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Contamination of pig carcass with Salmonella enterica serovar Typhimurium monophasic variant 1,4 [5], 12: i:- originates mainly in live animals es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.scitotenv.2019.134609 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Universidad CEU Cardenal Herrera//INDI 18%2F19/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/Universidad CEU Cardenal Herrera//IDOC 18%2F12/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Marin, C.; Chinillac, MC.; Cerda-Cuellar, M.; Montoro-Dasí, L.; Sevilla-Navarro, S.; Marco-Jiménez, F.; Ayats, T.... (2020). Contamination of pig carcass with Salmonella enterica serovar Typhimurium monophasic variant 1,4 [5], 12: i:- originates mainly in live animals. Science of The Total Environment. 703:1-7. https://doi.org/10.1016/j.scitotenv.2019.134609 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.scitotenv.2019.134609 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 7 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 703 es_ES
dc.identifier.pmid 31753504 es_ES
dc.relation.pasarela S\400359 es_ES
dc.contributor.funder Universidad CEU Cardenal Herrera es_ES
dc.description.references Amass, S.F, 2005a. Biosecurity: stopping the bugs from getting in. Pig J. 55, 104–114. Available online in: https://thepigsite.com/articles/biosecurity-stopping-the-bugs-from-getting-in-part-1-of-2 (accessed: 17th March, 2017). es_ES
dc.description.references Andres, V. M., & Davies, R. H. (2015). Biosecurity Measures to Control Salmonella and Other Infectious Agents in Pig Farms: A Review. Comprehensive Reviews in Food Science and Food Safety, 14(4), 317-335. doi:10.1111/1541-4337.12137 es_ES
dc.description.references ARGUELLO, H., ÁLVAREZ-ORDOÑEZ, A., CARVAJAL, A., RUBIO, P., & PRIETO, M. (2013). Role of Slaughtering in Salmonella Spreading and Control in Pork Production. Journal of Food Protection, 76(5), 899-911. doi:10.4315/0362-028x.jfp-12-404 es_ES
dc.description.references Arguello, H., Carvajal, A., Collazos, J. A., García-Feliz, C., & Rubio, P. (2012). Prevalence and serovars of Salmonella enterica on pig carcasses, slaughtered pigs and the environment of four Spanish slaughterhouses. Food Research International, 45(2), 905-912. doi:10.1016/j.foodres.2011.04.017 es_ES
dc.description.references Arguello, H., Carvajal, A., Naharro, G., Arcos, M., Rodicio, M. R., Martin, M. C., & Rubio, P. (2013). Sero- and genotyping of Salmonella in slaughter pigs, from farm to cutting plant, with a focus on the slaughter process. International Journal of Food Microbiology, 161(1), 44-52. doi:10.1016/j.ijfoodmicro.2012.11.013 es_ES
dc.description.references Baptista, F. M., Alban, L., Nielsen, L. R., Domingos, I., Pomba, C., & Almeida, V. (2010). Use of Herd Information for Predicting Salmonella Status in Pig Herds. Zoonoses and Public Health, 57, 49-59. doi:10.1111/j.1863-2378.2010.01354.x es_ES
dc.description.references Baptista, F. M., Dahl, J., & Nielsen, L. R. (2010). Factors influencing Salmonella carcass prevalence in Danish pig abattoirs. Preventive Veterinary Medicine, 95(3-4), 231-238. doi:10.1016/j.prevetmed.2010.04.007 es_ES
dc.description.references Bonardi, S., Bassi, L., Brindani, F., D’Incau, M., Barco, L., Carra, E., & Pongolini, S. (2013). Prevalence, characterization and antimicrobial susceptibility of Salmonella enterica and Yersinia enterocolitica in pigs at slaughter in Italy. International Journal of Food Microbiology, 163(2-3), 248-257. doi:10.1016/j.ijfoodmicro.2013.02.012 es_ES
dc.description.references Botteldoorn, N., Heyndrickx, M., Rijpens, N., Grijspeerdt, K., & Herman, L. (2003). Salmonella on pig carcasses: positive pigs and cross contamination in the slaughterhouse. Journal of Applied Microbiology, 95(5), 891-903. doi:10.1046/j.1365-2672.2003.02042.x es_ES
dc.description.references Campos, J., Mourão, J., Peixe, L., & Antunes, P. (2019). Non-typhoidal Salmonella in the Pig Production Chain: A Comprehensive Analysis of Its Impact on Human Health. Pathogens, 8(1), 19. doi:10.3390/pathogens8010019 es_ES
dc.description.references Casanova-Higes, A., Marín-Alcalá, C. M., Andrés-Barranco, S., Cebollada-Solanas, A., Alvarez, J., & Mainar-Jaime, R. C. (2019). Weaned piglets: another factor to be considered for the control of Salmonella infection in breeding pig farms. Veterinary Research, 50(1). doi:10.1186/s13567-019-0666-7 es_ES
dc.description.references Colello, R., Ruiz, M. J., Padín, V. M., Rogé, A. D., Leotta, G., Padola, N. L., & Etcheverría, A. I. (2018). Detection and Characterization of Salmonella Serotypes in the Production Chain of Two Pig Farms in Buenos Aires Province, Argentina. Frontiers in Microbiology, 9. doi:10.3389/fmicb.2018.01370 es_ES
dc.description.references Dang-Xuan, S., Nguyen-Viet, H., Pham-Duc, P., Unger, F., Tran-Thi, N., Grace, D., & Makita, K. (2019). Risk factors associated with Salmonella spp. prevalence along smallholder pig value chains in Vietnam. International Journal of Food Microbiology, 290, 105-115. doi:10.1016/j.ijfoodmicro.2018.09.030 es_ES
dc.description.references Davies, R., Gosling, R. ., Wales, A. D., & Smith, R. P. (2016). Use of an attenuated live Salmonella Typhimurium vaccine on three breeding pig units: A longitudinal observational field study. Comparative Immunology, Microbiology and Infectious Diseases, 46, 7-15. doi:10.1016/j.cimid.2016.03.005 es_ES
dc.description.references De Busser, E. V., De Zutter, L., Dewulf, J., Houf, K., & Maes, D. (2013). Salmonella control in live pigs and at slaughter. The Veterinary Journal, 196(1), 20-27. doi:10.1016/j.tvjl.2013.01.002 es_ES
dc.description.references Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010. Official Journal of European Union (DOI 20.10.2010). L276/33-79. es_ES
dc.description.references Duggan, S.J., Mannion, C., Prendergast, D.M., Leonard, N., Fanning, S., Gonzales-Barron, U., Egan, J., Butler, F., Duffy, G., 2010. Tracking the Salmonella status of pigs and pork from lairage through the slaughter process in the Republic of Ireland. J. Food Prot. 73, 2148–2160. https://doi.org/10.4315/0362-028x-73.12.2148. es_ES
dc.description.references Gomes-Neves, E., Antunes, P., Tavares, A., Themudo, P., Cardoso, M. F., Gärtner, F., … Peixe, L. (2012). Salmonella cross-contamination in swine abattoirs in Portugal: Carcasses, meat and meat handlers. International Journal of Food Microbiology, 157(1), 82-87. doi:10.1016/j.ijfoodmicro.2012.04.015 es_ES
dc.description.references Grimont, P., Weill, F.X., 2007. Antigenic Formulae of the Salmonella servovars. 9th ed. WHO Collaborating Center for Reference and Research on Salmonella (WHOCC-Salm). Institut Pasteur, Paris. es_ES
dc.description.references Hurd, H. S., McKean, J. D., Griffith, R. W., Wesley, I. V., & Rostagno, M. H. (2002). Salmonella enterica Infections in Market Swine with and without Transport and Holding. Applied and Environmental Microbiology, 68(5), 2376-2381. doi:10.1128/aem.68.5.2376-2381.2002 es_ES
dc.description.references ISO 6579:2002 (Anexo D), 2002. Microbiology of Food and Animal Feeding Stuffs. Horizontal Method for the Detection of Salmonella spp. International Organization for Standardization, Geneve, Switzerland. es_ES
dc.description.references MAGRAMA (Ministerio de Agricultura y Pesca, Alimentación y Medio Ambiente), 2016. http://www.mapama.gob.es/es/ministerio/servicios/informacion/plataforma-de-conocimiento-para-el-medio-rural-y-pesquero/observatorio-de-tecnologias-probadas/sistemas-prodnut-animal/ganado-porcino.aspx (accessed: 28th December, 2016). es_ES
dc.description.references Mannion, C., Fanning, J., McLernon, J., Lendrum, L., Gutierrez, M., Duggan, S., & Egan, J. (2012). The role of transport, lairage and slaughter processes in the dissemination of Salmonella spp. in pigs in Ireland. Food Research International, 45(2), 871-879. doi:10.1016/j.foodres.2011.02.001 es_ES
dc.description.references Marin, C., & Lainez, M. (2009). Salmonella detection in feces during broiler rearing and after live transport to the slaughterhouse. Poultry Science, 88(9), 1999-2005. doi:10.3382/ps.2009-00040 es_ES
dc.description.references Marquer, P., Rabade, T., Forti, R., 2014. Pig farming in the European Union, considerable variations from one Member State to another. Eurostat Stat. Focus 15, 1–12. http://ec.europa.eu/eurostat/statisticsexplained/index.php/Pig_farming_sector_-_statistical_portrait_2014 (accessed: 1st March, 2017). es_ES
dc.description.references Moré, E., Ayats, T., Ryan, P. G., Naicker, P. R., Keddy, K. H., Gaglio, D., … Cerdà-Cuéllar, M. (2017). Seabirds (Laridae) as a source ofCampylobacter spp.,Salmonellaspp. and antimicrobial resistance in South Africa. Environmental Microbiology, 19(10), 4164-4176. doi:10.1111/1462-2920.13874 es_ES
dc.description.references MULDER, R. W. A. W. (1995). IMPACT OF TRANSPORT AND RELATED STRESSES ON THE INCIDENCE AND EXTENT OF HUMAN PATHOGENS IN PIGMEAT AND POULTRY. Journal of Food Safety, 15(3), 239-246. doi:10.1111/j.1745-4565.1995.tb00136.x es_ES
dc.description.references Pesciaroli, M., Cucco, L., De Luca, S., Massacci, F. R., Maresca, C., Medici, L., … Magistrali, C. F. (2017). Association between pigs with high caecal Salmonella loads and carcass contamination. International Journal of Food Microbiology, 242, 82-86. doi:10.1016/j.ijfoodmicro.2016.11.021 es_ES
dc.description.references Schut, C. H., Farzan, A., Ainslie-Garcia, M. H., Friendship, R. M., & Lillie, B. N. (2019). Antibody Responses to Salmonella in Pigs from Weaning Up to Marketing and Presence of Salmonella at Slaughter. Foodborne Pathogens and Disease, 16(3), 187-194. doi:10.1089/fpd.2018.2454 es_ES
dc.description.references Vidic, B., Savic, S., Prica, N., & Suvajdzic, L. (2015). Epizootiology and Control Measures for Salmonella in Pigs. Procedia Food Science, 5, 312-315. doi:10.1016/j.profoo.2015.09.067 es_ES
dc.description.references Visscher, C. F., Klein, G., Verspohl, J., Beyerbach, M., Stratmann-Selke, J., & Kamphues, J. (2011). Serodiversity and serological as well as cultural distribution of Salmonella on farms and in abattoirs in Lower Saxony, Germany. International Journal of Food Microbiology, 146(1), 44-51. doi:10.1016/j.ijfoodmicro.2011.01.038 es_ES


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