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Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations

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Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations

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dc.contributor.author Todea Morar, Irina Maria es_ES
dc.contributor.author Rensen, Stephanie es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.contributor.author Boscaiu, Monica es_ES
dc.contributor.author Holonec, Liviu es_ES
dc.contributor.author Sestras, Adriana F. es_ES
dc.contributor.author Vicente, Oscar es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.contributor.author Sestras, Radu E. es_ES
dc.contributor.author Plazas Ávila, María de la O es_ES
dc.date.accessioned 2021-09-16T03:32:04Z
dc.date.available 2021-09-16T03:32:04Z
dc.date.issued 2020-05 es_ES
dc.identifier.uri http://hdl.handle.net/10251/172601
dc.description.abstract [EN] Assessment of the diversity of reproductive traits and genetic variation is of great relevance to the conservation of genetic resources and management of silver fir (Abies alba) populations. We have evaluated reproductive characteristics associated with female cones and seed morphology, as well as seed germination after subjecting seeds to five storage methods, in nine Romanian populations of A. alba. The genetic diversity of the populations was assessed with 12 polymorphic simple sequence repeat (SSR) markers. We detected significant differences between populations for all reproductive traits and considerable differences in seed germination and storage methods; seed storage in wet sand was the method resulting in the highest germination in all populations. Genomic SSRs (gSSRs) were more informative on average than expressed sequence tag SSRs (EST-SSRs) in the populations studied. The nine populations were genetically diverse, with an average number of alleles (N) per SSR locus between 3.50 and 4.83. The observed heterozygosity (Ho) in the nine populations was always lower than the expected heterozygosity (He), which resulted in values of the inbreeding coefficient (Fis) between 0.261 and 0.709. Genetic distances between populations ranged between 0.077 and 0.410. The cluster analysis based on genetic distances did not group accessions according to their geographical proximity, and despite a positive trend, the correlation between geographic and genetic distances was non-significant. The results of an analysis of molecular variance (AMOVA) revealed that only 9.1% of the total molecular variance is attributable to differences between populations. This low degree of genetic differentiation between populations is confirmed by the intermingling of individuals of different populations in a principal coordinate analysis (PCoA). We found evidence of a positive relationship between He and germination, as well as a negative one between Fis and germination, suggesting that populations with low diversity and high consanguinity may have a reduced fitness and long-term viability. The results are relevant for the conservation and management of local genetic resources and populations, as well as for reforestation programmes of silver fir. es_ES
dc.description.sponsorship The publication was supported by funds from the National Research Development Projects to Finance Excellence (PFE)-37/2018-2020 granted by the Romanian Ministry of Research and Innovation. The first author (I.M.T.M.) is thankful to UPV (Valencia) and the Erasmus+ mobility programme, financed by the European Commission, for her scholarship in UPV. The research was partially supported by the Advanced Horticultural Research Institute of Transylvania (ICHAT), University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca (USAMVCN), and Polytechnic University of Valencia (UPV). Mariola Plazas is grateful to Generalitat Valenciana and Fondo Social Europeo for a post-doctoral grant (APOSTD/2018/014). es_ES
dc.language Inglés es_ES
dc.publisher MDPI AG es_ES
dc.relation.ispartof Sustainability es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Silver fir es_ES
dc.subject Abies alba es_ES
dc.subject Germination es_ES
dc.subject SSRs es_ES
dc.subject Genetic diversity es_ES
dc.subject Inbreeding es_ES
dc.subject.classification GENETICA es_ES
dc.subject.classification BOTANICA es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.3390/su12104199 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MCI//37%2F2018-2020/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//APOSTD%2F2018%2F014/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ecosistemas Agroforestales - Departament d'Ecosistemes Agroforestals es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Todea Morar, IM.; Rensen, S.; Vilanova Navarro, S.; Boscaiu, M.; Holonec, L.; Sestras, AF.; Vicente, O.... (2020). Genetic Relationships and Reproductive Traits of Romanian Populations of Silver Fir (Abies alba): Implications for the Sustainable Management of Local Populations. Sustainability. 12(10):1-15. https://doi.org/10.3390/su12104199 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.3390/su12104199 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 15 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 12 es_ES
dc.description.issue 10 es_ES
dc.identifier.eissn 2071-1050 es_ES
dc.relation.pasarela S\412739 es_ES
dc.contributor.funder European Social Fund es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Universitat Politècnica de València es_ES
dc.contributor.funder Ministry of Research and Innovation, Rumanía es_ES
dc.contributor.funder University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca es_ES
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