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Long-term and transgenerational phenotypic, transcriptional and metabolic effects in rabbit males born following vitrified embryo transfer

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Long-term and transgenerational phenotypic, transcriptional and metabolic effects in rabbit males born following vitrified embryo transfer

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dc.contributor.author Garcia-Dominguez, X es_ES
dc.contributor.author Marco-Jiménez, Francisco es_ES
dc.contributor.author Peñaranda, D.S. es_ES
dc.contributor.author Diretto, Gianfranco es_ES
dc.contributor.author García-Carpintero, Víctor es_ES
dc.contributor.author Cañizares Sales, Joaquín es_ES
dc.contributor.author Vicente Antón, José Salvador es_ES
dc.date.accessioned 2021-11-05T10:17:55Z
dc.date.available 2021-11-05T10:17:55Z
dc.date.issued 2020-07-09 es_ES
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10251/176080
dc.description.abstract [EN] The advent of assisted reproductive technologies (ART) in mammals involved an extraordinary change in the environment where the beginning of a new organism takes place. Under in vitro conditions, in which ART is currently being performed, it likely fails to mimic optimal in vivo conditions. This suboptimal environment could mediate in the natural developmental trajectory of the embryo, inducing lasting effects until later life stages that may be inherited by subsequent generations (transgenerational effects). Therefore, we evaluated the potential transgenerational effects of embryo exposure to the cryopreservation-transfer procedure in a rabbit model on the offspring phenotype, molecular physiology of the liver (transcriptome and metabolome) and reproductive performance during three generations (F1, F2 and F3). The results showed that, compared to naturally-conceived animals (NC group), progeny generated after embryo exposure to the cryopreservation-transfer procedure (VT group) exhibited lower body growth, which incurred lower adult body weight in the F1 (direct effects), F2 (intergenerational effects) and F3 (transgenerational effects) generations. Furthermore, VT animals showed intergenerational effects on heart weight and transgenerational effects on liver weight. The RNA-seq data of liver tissue revealed 642 differentially expressed transcripts (DETs) in VT animals from the F1 generation. Of those, 133 were inherited from the F2 and 120 from the F3 generation. Accordingly, 151, 190 and 159 differentially accumulated metabolites (DAMs) were detected from the F1, F2 and F3, respectively. Moreover, targeted metabolomics analysis demonstrated that transgenerational effects were mostly presented in the non-polar fraction. Functional analysis of molecular data suggests weakened zinc and fatty acid metabolism across the generations, associated with alterations in a complex molecular network affecting global hepatic metabolism that could be associated with the phenotype of VT animals. However, these VT animals showed proper reproductive performance, which verified a functional health status. In conclusion, our results establish the long-term transgenerational effects following a vitrified embryo transfer procedure. We showed that the VT phenotype could be the result of the manifestation of embryonic developmental plasticity in response to the stressful conditions during ART procedures. es_ES
dc.description.sponsorship Funding from the Ministry of Economy, Industry and Competitiveness (Research project: AGL2014-53405-C2-1-P) and Generalitat Valenciana (Research project: Prometeo II 2014/036) is acknowledged. X.G.D. was supported by a research grant from the Ministry of Economy, Industry and Competitiveness (BES-2015-072429). English text version was revised by N. Macowan English Language Service. es_ES
dc.language Inglés es_ES
dc.publisher Nature Publishing Group es_ES
dc.relation.ispartof Scientific Reports es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.subject.classification GENETICA es_ES
dc.subject.classification BIOLOGIA ANIMAL es_ES
dc.title Long-term and transgenerational phenotypic, transcriptional and metabolic effects in rabbit males born following vitrified embryo transfer es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1038/s41598-020-68195-9 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GENERALITAT VALENCIANA//PROMETEOII%2F2014%2F036//Crioconservación de óvulos y embriones. Cambios epigenéticos y repercusiones transcripcionales y proteómicas / es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINISTERIO DE ECONOMIA Y EMPRESA//AGL2014-53405-C2-1-P//MEJORA GENETICA DEL CONEJO DE CARNE:RESPUESTA A LA SELECCION Y SU EFECTO SOBRE LA REPRODUCCION, ALIMENTACION Y SALUD UTILIZANDO UNA POBLACION CONTROL CRIOCONSERVADA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BES-2015-072429//AYUDA CONTRATO PREDOCTORAL PARA LA FORMACION DE DOCTORES-GARCIA DOMINGUEZ/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Garcia-Dominguez, X.; Marco-Jiménez, F.; Peñaranda, D.; Diretto, G.; García-Carpintero, V.; Cañizares Sales, J.; Vicente Antón, JS. (2020). Long-term and transgenerational phenotypic, transcriptional and metabolic effects in rabbit males born following vitrified embryo transfer. Scientific Reports. 10(1):1-15. https://doi.org/10.1038/s41598-020-68195-9 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1038/s41598-020-68195-9 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 15 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 10 es_ES
dc.description.issue 1 es_ES
dc.identifier.pmid 32647175 es_ES
dc.identifier.pmcid PMC7347584 es_ES
dc.relation.pasarela S\414277 es_ES
dc.contributor.funder GENERALITAT VALENCIANA es_ES
dc.contributor.funder MINISTERIO DE ECONOMIA Y EMPRESA es_ES
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